Restriction map for
pFN21AE2286
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AvrII
BalI BamHI BbeI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BpmI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DraI EaeI EagI
Eam1105I EclXI Eco52I EcoRI EcoT22I
FauI FseI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI StuI SwaI TaqI TthHB8I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccII 121 CGCG CG^CG
AciI 122 GCGG CCGC(-3/-1)
AocI 1177 CCTGAGG CC^T[ACGT]AGG
Aor51HI 106 AGCGCT AGC^GCT
AspI 720 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 918 CCCGAG C^[CT]CG[GA]G
AviII 806 TGCGCA TGC^GCA
BbrPI 130 CACGTG CAC^GTG
BbsI 187 GAAGAC GAAGAC(2/6)
BbvI 1108 GCTGC GCAGC(8/12)
BclI 341 TGATCA T^GATCA
BlpI 1300 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1300 GCTAAGC GC^T[ACGT]AGC
BpuAI 187 GAAGAC GAAGAC(2/6)
BsaAI 130 CACGTG [CT]AC^GT[GA]
BsaI 522 GGTCTC GGTCTC(1/5)
BsoBI 918 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1280 TGTACA T^GTACA
BspMI 1013 ACCTGC ACCTGC(4/8)
BsrDI 426 GCAATG GCAATG(2/0)
BsrFI 55 GCCGGT [GA]^CCGG[CT]
BsrGI 1280 TGTACA T^GTACA
BstEII 1000 GGTAACC G^GT[ACGT]ACC
BstPI 1000 GGTAACC G^GT[ACGT]ACC
BstUI 121 CGCG CG^CG
BstXI 104 CCAGCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1177 CCTGAGG CC^T[ACGT]AGG
CelII 1300 GCTAAGC GC^T[ACGT]AGC
Cfr10I 55 GCCGGT [GA]^CCGG[CT]
DraIII 309 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 640 GACATCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 106 AGCGCT AGC^GCT
Eco81I 1177 CCTGAGG CC^T[ACGT]AGG
EcoNI 118 CCTCGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1000 GGTAACC G^GT[ACGT]ACC
EcoRV 439 GATATC GAT^ATC
FbaI 341 TGATCA T^GATCA
Fnu4HI 1108 GCTGC GC^[ACGT]GC
FspI 806 TGCGCA TGC^GCA
HaeII 106 AGCGCT [GA]GCGC^[CT]
HincII 814 GTCAAC GT[CT]^[GA]AC
HindII 814 GTCAAC GT[CT]^[GA]AC
ItaI 1108 GCTGC GC^[ACGT]GC
MseI 164 TTAA T^TAA
MslI 64 CACATGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 121 CGCG CG^CG
MwoI 734 GCTCCCGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 659 CATATG CA^TATG
PflFI 720 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 308 CCACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 130 CACGTG CAC^GTG
PmlI 130 CACGTG CAC^GTG
SacI 741 GAGCTC GAGCT^C
SbfI 1129 CCTGCAGG CCTGCA^GG
Sse8387I 1129 CCTGCAGG CCTGCA^GG
SspBI 1280 TGTACA T^GTACA
SspI 222 AATATT AAT^ATT
TfiI 194 GAATC G^A[AT]TC
Tru9I 164 TTAA T^TAA
TseI 1108 GCTGC G^C[AT]GC
Tth111I 720 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 308 CCACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AfaI 756 GTAC GT^AC
1281 GTAC
ApaI 690 GGGCCC GGGCC^C
846 GGGCCC
Asp700I 365 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
950 GAAACCCTTC
BanI 114 GGTGCC G^G[CT][GA]CC
321 GGCACC
BcnI 688 CCGGG CC^[GC]GG
737 CCCGG
BsgI 463 CTGCAC GTGCAG(16/14)
1015 CTGCAC
BsmAI 523 GTCTC GTCTC(1/5)
1321 GTCTC
BspLU11I 280 ACATGT A^CATGT
752 ACATGT
BsrI 310 ACTGG ACTGG(1/-1)
626 CCAGT
Cac8I 558 GCTTGC GC[ACGT]^[ACGT]GC
1128 GCCTGC
CfoI 107 GCGC GCG^C
807 GCGC
HhaI 107 GCGC GCG^C
807 GCGC
HinP1I 107 GCGC G^CGC
807 GCGC
NciI 688 CCGGG CC^[GC]GG
737 CCCGG
NcoI 213 CCATGG C^CATGG
335 CCATGG
PstI 800 CTGCAG CTGCA^G
1130 CTGCAG
RsaI 756 GTAC GT^AC
1281 GTAC
Tsp509I 169 AATT ^AATT
535 AATT
XmnI 365 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
950 GAAACCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AflIII AluI AlwI AspHI AvaII
BanII BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FokI HaeIII HapII
HinfI HpaII HphI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MspI MvaI NdeII NlaIII NlaIV
NspI PleI PpuMI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI Tsp45I
TspRI XcmI XhoII