##################################
# NO CUT ENZYMES (  203 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        AlwI        AocI        Aor51HI     
ApaI        ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DpnII       
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HapII       HgaI        Hin1I       
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspA1I      MspI        MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        TfiI        Tru9I       Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AfaI          120  GTAC                 GT^AC
AluI          206  AGCT                 AG^CT
Alw44I        212  GTGCAC               G^TGCAC
AlwNI         250  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         212  GTGCAC               G^TGCAC
AspHI         212  GTGCAC               G[AT]GC[AT]^C
AvaII         263  GGACC                G^G[AT]CC
BbsI           79  GAAGAC               GAAGAC(2/6)
BmyI          212  GTGCAC               G[AGT]GC[ACT]^C
BpuAI          79  GAAGAC               GAAGAC(2/6)
BsiHKAI       212  GTGCAC               G[AT]GC[AT]^C
BsmAI         252  GAGAC                GTCTC(1/5)
Bsp1286I      212  GTGCAC               G[AGT]GC[ACT]^C
BsrI           12  ACTGG                ACTGG(1/-1)
CfoI          282  GCGC                 GCG^C
DraII         262  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO109I      262  AGGACCT              [GA]G^G[ACGT]CC[CT]
HaeII         281  AGCGCC               [GA]GCGC^[CT]
HhaI          282  GCGC                 GCG^C
HinP1I        282  GCGC                 G^CGC
HphI          185  TCACC                GGTGA(8/7)
MaeIII         97  GTGAC                ^GT[ACGT]AC
MboII          79  GAAGA                GAAGA(8/7)
PpuMI         262  AGGACCT              [GA]G^G[AT]CC[CT]
RsaI          120  GTAC                 GT^AC
Tsp45I         97  GTGAC                ^GT[GC]AC
TspRI         191  CACTG                CA[GC]TG[ACGT][ACGT]^
XcmI          220  CCAGACCAGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AciI          201  CCGC                 CCGC(-3/-1)
              285  CCGC                
BanI           64  GGCACC               G^G[CT][GA]CC
              168  GGTGCC              
BbvCI          18  CCTCAGC              CCTCAGC(-5/-2)
              266  CCTCAGC             
BssKI         115  CCTGG                ^CC[ACGT]GG
              230  CCTGG               
BstNI         115  CCTGG                CC^[AT]GG
              230  CCTGG               
EcoRII        115  CCTGG                ^CC[AT]GG
              230  CCTGG               
MvaI          115  CCTGG                CC^[AT]GG
              230  CCTGG               
PstI          162  CTGCAG               CTGCA^G
              256  CTGCAG              
ScrFI         115  CCTGG                CC^[ACGT]GG
              230  CCTGG               
SfaNI          73  GATGC                GCATC(5/9)
              242  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

BbvI        BsaJI       BsiYI       BslI        BstF5I      
Cac8I       Cfr13I      DdeI        Fnu4HI      FokI        
HaeIII      ItaI        MnlI        NlaIV       Sau96I      
SfcI        TseI