Restriction map for
pFN21AE1508
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AviII AvrII BalI BamHI BanII
BbrPI BbsI BcgI BclI BfaI
BfrI BglI BlnI BmyI BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsiHKAI BsiWI BsmBI BsmI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BsrI BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraII DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MaeI
MaeII MamI MfeI MluI MluNI
MroI MscI MseI MspA1I MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AcyI 235 GGCGCC G[GA]^CG[CT]C
AfaI 420 GTAC GT^AC
AspI 283 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 150 CCCGGG C^[CT]CG[GA]G
BbeI 235 GGCGCC GGCGC^C
BbvCI 102 CCTCAGC CCTCAGC(-5/-2)
BbvI 258 GCTGC GCAGC(8/12)
BciVI 397 GTATCC GTATCC(6/5)
BglII 50 AGATCT A^GATCT
BlpI 249 GCTGAGC GC^T[ACGT]AGC
BpmI 88 CTGGAG CTGGAG(16/14)
Bpu1102I 249 GCTGAGC GC^T[ACGT]AGC
BsaHI 235 GGCGCC G[GA]^CG[CT]C
BsoBI 150 CCCGGG C^[CT]CG[GA]G
BspMI 143 ACCTGC ACCTGC(4/8)
BstEII 321 GGTCACC G^GT[ACGT]ACC
BstF5I 270 CATCC GGATG(2/0)
BstPI 321 GGTCACC G^GT[ACGT]ACC
BstXI 182 CCAAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 50 AGATCT [GA]^GATC[CT]
CelII 249 GCTGAGC GC^T[ACGT]AGC
DpnII 51 GATC ^GATC
DsaI 197 CCGTGG C^C[GA][CT]GG
EagI 356 CGGCCG C^GGCCG
EclXI 356 CGGCCG C^GGCCG
Eco52I 356 CGGCCG C^GGCCG
EcoO65I 321 GGTCACC G^GT[ACGT]ACC
EcoT14I 182 CCAAGG C^C[AT][AT]GG
FokI 270 CATCC GGATG(9/13)
HaeII 235 GGCGCC [GA]GCGC^[CT]
HgaI 205 GACGC GACGC(5/10)
Hin1I 235 GGCGCC G[GA]^CG[CT]C
HincII 289 GTCAAC GT[CT]^[GA]AC
HindII 289 GTCAAC GT[CT]^[GA]AC
HinfI 84 GACTC G^A[ACGT]TC
KasI 235 GGCGCC G^GCGCC
MaeIII 322 GTCAC ^GT[ACGT]AC
MboI 51 GATC ^GATC
MflI 50 AGATCT [GA]^GATC[CT]
MslI 27 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 235 GGCGCC GG^CGCC
NdeII 51 GATC ^GATC
NlaIII 39 CATG CATG^
PflFI 283 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 84 GACTC GAGTC(4/5)
RsaI 420 GTAC GT^AC
Sau3AI 51 GATC ^GATC
SfaNI 218 GCATC GCATC(5/9)
SfoI 235 GGCGCC GGC^GCC
SmaI 150 CCCGGG CCC^GGG
StyI 182 CCAAGG C^C[AT][AT]GG
TseI 258 GCTGC G^C[AT]GC
Tsp45I 322 GTCAC ^GT[GC]AC
TspRI 310 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 283 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 50 AGATCT [GA]^GATC[CT]
XmaCI 150 CCCGGG C^CCGGG
XmaI 150 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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BanI 76 GGCACC G^G[CT][GA]CC
235 GGCGCC
BsgI 145 CTGCAC GTGCAG(16/14)
259 CTGCAC
BsiEI 356 CGGCCG CG[GA][CT]^CG
392 CGGTCG
BsiYI 144 CCTGCACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
273 CCTGCGCGTGG
BslI 144 CCTGCACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
273 CCTGCGCGTGG
BsmAI 82 GAGAC GTCTC(1/5)
3 GTCTC
BsmFI 377 GGGAC GGGAC(10/14)
306 GTCCC
BstNI 327 CCTGG CC^[AT]GG
374 CCTGG
EarI 97 CTCTTC CTCTTC(1/4)
213 GAAGAG
EcoRII 327 CCTGG ^CC[AT]GG
374 CCTGG
HapII 151 CCGG C^CGG
286 CCGG
HpaII 151 CCGG C^CGG
286 CCGG
HphI 210 GGTGA GGTGA(8/7)
323 TCACC
Ksp632I 97 CTCTTC CTCTTC(1/4)
213 GAAGAG
MboII 213 GAAGA GAAGA(8/7)
98 TCTTC
MspI 151 CCGG C^CGG
286 CCGG
MvaI 327 CCTGG CC^[AT]GG
374 CCTGG
SapI 213 GAAGAGC GCTCTTC(1/4)
96 GCTCTTC
TaqI 140 TCGA T^CGA
221 TCGA
TthHB8I 140 TCGA T^CGA
221 TCGA
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AccII AciI AluI AvaII BcnI
BsaJI BssHII BssKI BstUI Cac8I
CfoI Cfr13I DdeI EaeI FauI
Fnu4HI HaeIII HhaI HinP1I ItaI
MnlI MvnI MwoI NciI NlaIV
Sau96I ScrFI