Restriction map for
pFN21AE1121
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BpuAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrDI BssHII Bst1107I
BstBI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MaeI MamI
MfeI MflI MluI MroI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI SspI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 37 enzymes) #
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Asp700I 525 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 737 GAGCTC G[AT]GC[AT]^C
BalI 44 TGGCCA TGG^CCA
BbrPI 166 CACGTG CAC^GTG
BlpI 567 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 567 GCTGAGC GC^T[ACGT]AGC
BseRI 145 GAGGAG GAGGAG(10/8)
BsiHKAI 737 GAGCTC G[AT]GC[AT]^C
BsmAI 958 GAGAC GTCTC(1/5)
Bsp1407I 803 TGTACA T^GTACA
BsrGI 803 TGTACA T^GTACA
BstEII 432 GGTGACC G^GT[ACGT]ACC
BstPI 432 GGTGACC G^GT[ACGT]ACC
CelII 567 GCTGAGC GC^T[ACGT]AGC
DpnII 978 GATC ^GATC
EarI 358 CTCTTC CTCTTC(1/4)
EcoO65I 432 GGTGACC G^GT[ACGT]ACC
HphI 432 GGTGA GGTGA(8/7)
Ksp632I 358 CTCTTC CTCTTC(1/4)
KspI 219 CCGCGG CCGC^GG
MboI 978 GATC ^GATC
MboII 359 TCTTC GAAGA(8/7)
MluNI 44 TGGCCA TGG^CCA
MscI 44 TGGCCA TGG^CCA
NdeII 978 GATC ^GATC
PmaCI 166 CACGTG CAC^GTG
PmlI 166 CACGTG CAC^GTG
SacI 737 GAGCTC GAGCT^C
SacII 219 CCGCGG CCGC^GG
SapI 357 GCTCTTC GCTCTTC(1/4)
Sau3AI 978 GATC ^GATC
SbfI 813 CCTGCAGG CCTGCA^GG
Sse8387I 813 CCTGCAGG CCTGCA^GG
SspBI 803 TGTACA T^GTACA
StuI 527 AGGCCT AGG^CCT
TspRI 943 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 525 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AfaI 180 GTAC GT^AC
804 GTAC
BbeI 640 GGCGCC GGCGC^C
675 GGCGCC
BsaAI 166 CACGTG [CT]AC^GT[GA]
181 TACGTG
BsrBI 213 CCGCTC CCGCTC(-3/-3)
516 GAGCGG
BsrI 839 ACTGG ACTGG(1/-1)
177 CCAGT
BssSI 511 CACGAG CACGAG(-5/-1)
1075 CTCGTG
DdeI 568 CTGAG C^T[ACGT]AG
740 CTCAG
DsaI 204 CCACGG C^C[GA][CT]GG
219 CCGCGG
HgaI 452 GCGTC GACGC(5/10)
634 GCGTC
HinfI 490 GACTC G^A[ACGT]TC
964 GACTC
KasI 640 GGCGCC G^GCGCC
675 GGCGCC
MslI 162 CACCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1035 CATGAGGGTG
NarI 640 GGCGCC GG^CGCC
675 GGCGCC
PflMI 177 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
645 CCACAAGCTGG
PleI 490 GACTC GAGTC(4/5)
964 GACTC
RsaI 180 GTAC GT^AC
804 GTAC
SfoI 640 GGCGCC GGC^GCC
675 GGCGCC
Van91I 177 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
645 CCACAAGCTGG
XcmI 326 CCAACAGCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1007 CCAGGCCCCAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
ApaI AvaI AvaII BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BsgI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII ItaI
MaeII MaeIII MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NgoMIV NlaIII NlaIV PstI PvuII
Sau96I ScrFI SfaNI SfcI SmaI
StyI TaqI TseI Tsp45I TthHB8I
XmaCI XmaI