Restriction map for
pFN21AE4531
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AvrII BamHI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI HindIII HpaI MamI MfeI
MluI MunI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SwaI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 1496 GGTACC G^GTACC
AccIII 714 TCCGGA T^CCGGA
Asp718I 1496 GGTACC G^GTACC
AspI 1669 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 158 TGCGCA TGC^GCA
BalI 1614 TGGCCA TGG^CCA
BbrPI 1240 CACGTG CAC^GTG
BseAI 714 TCCGGA T^CCGGA
BsgI 1237 CTGCAC GTGCAG(16/14)
BsmFI 213 GTCCC GGGAC(10/14)
BspEI 714 TCCGGA T^CCGGA
BssSI 1636 CTCGTG CACGAG(-5/-1)
BstAPI 1626 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1286 AGATCC [GA]^GATC[CT]
DraIII 205 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1552 GACTTCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 257 CGGCCG C^GGCCG
EclXI 257 CGGCCG C^GGCCG
Eco52I 257 CGGCCG C^GGCCG
EcoNI 1048 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 379 GGCCGGCC GGCCGG^CC
FspI 158 TGCGCA TGC^GCA
HgaI 560 GCGTC GACGC(5/10)
HincII 1273 GTCAAC GT[CT]^[GA]AC
HindII 1273 GTCAAC GT[CT]^[GA]AC
KpnI 1496 GGTACC GGTAC^C
KspI 243 CCGCGG CCGC^GG
MflI 1286 AGATCC [GA]^GATC[CT]
MluNI 1614 TGGCCA TGG^CCA
MroI 714 TCCGGA T^CCGGA
MscI 1614 TGGCCA TGG^CCA
NcoI 982 CCATGG C^CATGG
NdeI 803 CATATG CA^TATG
PflFI 1669 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1240 CACGTG CAC^GTG
PmlI 1240 CACGTG CAC^GTG
PshAI 45 GACATCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 294 CAGCTG CAG^CTG
SacI 904 GAGCTC GAGCT^C
SacII 243 CCGCGG CCGC^GG
SapI 1566 GAAGAGC GCTCTTC(1/4)
ScaI 473 AGTACT AGT^ACT
SexAI 437 ACCAGGT A^CC[AT]GGT
SfiI 238 GGCCTCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1378 CTCAAG C^T[CT][GA]AG
SphI 1604 GCATGC GCATG^C
SspI 920 AATATT AAT^ATT
StuI 1298 AGGCCT AGG^CCT
Tsp509I 537 AATT ^AATT
Tth111I 1669 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1291 CCACCACAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1286 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 1287 GATCC GGATC(4/5)
1656 GATCC
AlwNI 439 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
967 CAGAGTCTG
AspHI 904 GAGCTC G[AT]GC[AT]^C
1569 GAGCAC
BbsI 1145 GAAGAC GAAGAC(2/6)
1171 GAAGAC
BpuAI 1145 GAAGAC GAAGAC(2/6)
1171 GAAGAC
BsaAI 463 TACGTG [CT]AC^GT[GA]
1240 CACGTG
BsaI 776 GAGACC GGTCTC(1/5)
509 GGTCTC
BsaWI 714 TCCGGA [AT]^CCGG[AT]
908 TCCGGT
BseRI 1049 CTCCTC GAGGAG(10/8)
994 GAGGAG
BsiEI 50 CGGTCG CG[GA][CT]^CG
257 CGGCCG
BsiHKAI 904 GAGCTC G[AT]GC[AT]^C
1569 GAGCAC
BsmI 850 GAATGC GAATGC(1/-1)
962 GAATGC
BsrFI 380 GCCGGC [GA]^CCGG[CT]
1305 GCCGGC
Cfr10I 380 GCCGGC [GA]^CCGG[CT]
1305 GCCGGC
MslI 459 CACCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1599 CACGGGCATG
NaeI 380 GCCGGC GCC^GGC
1305 GCCGGC
NgoMIV 380 GCCGGC G^CCGGC
1305 GCCGGC
NspI 746 ACATGC [GA]CATG^[CT]
1604 GCATGC
PflMI 438 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1386 CCAGCCCTTGG
PpuMI 815 AGGACCC [GA]G^G[AT]CC[CT]
1201 GGGTCCT
PstI 297 CTGCAG CTGCA^G
1736 CTGCAG
SmaI 263 CCCGGG CCC^GGG
1581 CCCGGG
TfiI 738 GAATC G^A[AT]TC
942 GATTC
Van91I 438 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1386 CCAGCCCTTGG
XmaCI 263 CCCGGG C^CCGGG
1581 CCCGGG
XmaI 263 CCCGGG C^CCGGG
1581 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AvaI AvaII BanI BanII
BbeI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaJI BsiYI
BslI BsmAI BsoBI Bsp1286I BspMI
BsrBI BsrI BssHII BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StyI TaqI Tru9I TseI Tsp45I
TspRI TthHB8I