Restriction map for
pFN21AE6134
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# NO CUT ENZYMES ( 195 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DpnII DraI DraII DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MaeII MamI
MboI MfeI MflI MluI MroI
MseI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NdeII
NgoMIV NheI NlaIV NotI NruI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tru9I
Tsp509I TspRI Tth111I TthHB8I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 31 GCGG CCGC(-3/-1)
AlwNI 157 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 94 GTGCTC G[AT]GC[AT]^C
BalI 282 TGGCCA TGG^CCA
BanII 381 GGGCTC G[GA]GC[CT]^C
BbvI 147 GCTGC GCAGC(8/12)
BfaI 403 CTAG C^TAG
BpmI 51 CTGGAG CTGGAG(16/14)
BsaJI 366 CCTTGG C^C[ACGT][ACGT]GG
BsiHKAI 94 GTGCTC G[AT]GC[AT]^C
BsmFI 215 GGGAC GGGAC(10/14)
BspMI 170 ACCTGC ACCTGC(4/8)
BstF5I 303 CATCC GGATG(2/0)
Cfr13I 78 GGCCC G^G[ACGT]CC
EcoT14I 366 CCTTGG C^C[AT][AT]GG
EcoT22I 7 ATGCAT ATGCA^T
Fnu4HI 147 GCTGC GC^[ACGT]GC
FokI 303 CATCC GGATG(9/13)
HphI 165 GGTGA GGTGA(8/7)
ItaI 147 GCTGC GC^[ACGT]GC
MaeI 403 CTAG C^TAG
MaeIII 275 GTCAC ^GT[ACGT]AC
MboII 17 TCTTC GAAGA(8/7)
MluNI 282 TGGCCA TGG^CCA
MnlI 179 CCTC CCTC(7/6)
MscI 282 TGGCCA TGG^CCA
MslI 303 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 29 CAGCGG C[AC]G^C[GT]G
MwoI 70 GCTGGCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 7 ATGCAT ATGCA^T
PflMI 156 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 217 GACTC GAGTC(4/5)
PstI 221 CTGCAG CTGCA^G
Sau96I 78 GGCCC G^G[ACGT]CC
SexAI 155 ACCAGGT A^CC[AT]GGT
SfcI 221 CTGCAG C^T[GA][CT]AG
StyI 366 CCTTGG C^C[AT][AT]GG
TfiI 232 GATTC G^A[AT]TC
TseI 147 GCTGC G^C[AT]GC
Tsp45I 275 GTCAC ^GT[GC]AC
Van91I 156 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 60 CCACAGTCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 9 enzymes) #
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AfaI 112 GTAC GT^AC
160 GTAC
BmyI 94 GTGCTC G[AGT]GC[ACT]^C
381 GGGCTC
BsmAI 205 GAGAC GTCTC(1/5)
288 GTCTC
Bsp1286I 94 GTGCTC G[AGT]GC[ACT]^C
381 GGGCTC
BsrI 162 ACTGG ACTGG(1/-1)
285 CCAGT
EaeI 282 TGGCCA [CT]^GGCC[GA]
335 TGGCCG
HinfI 217 GACTC G^A[ACGT]TC
232 GATTC
RsaI 112 GTAC GT^AC
160 GTAC
SfaNI 6 GATGC GCATC(5/9)
125 GCATC
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AluI BsiYI BslI BssKI BstNI
Cac8I DdeI EcoRII HaeIII MvaI
NlaIII ScrFI