Restriction map for
pFN21AE1326
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AccI AclI AcsI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
AspEI AspHI AviII BbeI BbrPI
BbvCI BciVI BfrI BglII BsaAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KasI KspI MfeI MluI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI ScaI SfoI SfuI SgrAI
SnaBI SpeI SspBI StuI SwaI
TfiI Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 802 GACGTC GACGT^C
Acc65I 1457 GGTACC G^GTACC
ApaI 767 GGGCCC GGGCC^C
Asp700I 1041 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1457 GGTACC G^GTACC
AspI 841 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1017 CCTAGG C^CTAGG
BalI 452 TGGCCA TGG^CCA
BamHI 494 GGATCC G^GATCC
BanI 1457 GGTACC G^G[CT][GA]CC
BbsI 1534 GTCTTC GAAGAC(2/6)
BclI 320 TGATCA T^GATCA
BlnI 1017 CCTAGG C^CTAGG
BpuAI 1534 GTCTTC GAAGAC(2/6)
BsaBI 1582 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 1700 TCATGA T^CATGA
BssSI 370 CACGAG CACGAG(-5/-1)
DraIII 594 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 960 GAAGAG CTCTTC(1/4)
FbaI 320 TGATCA T^GATCA
KpnI 1457 GGTACC GGTAC^C
Ksp632I 960 GAAGAG CTCTTC(1/4)
MamI 1582 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 452 TGGCCA TGG^CCA
MscI 452 TGGCCA TGG^CCA
NspI 1263 GCATGC [GA]CATG^[CT]
PflFI 841 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1013 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 1700 TCATGA T^CATGA
SbfI 498 CCTGCAGG CCTGCA^GG
SexAI 1649 ACCTGGT A^CC[AT]GGT
SfiI 123 GGCCAGTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 310 CTCAAG C^T[CT][GA]AG
SphI 1263 GCATGC GCATG^C
Sse8387I 498 CCTGCAGG CCTGCA^GG
SspI 616 AATATT AAT^ATT
Tth111I 841 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1041 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 572 TCCGGA T^CCGGA
906 TCCGGA
AcyI 730 GGCGTC G[GA]^CG[CT]C
802 GACGTC
BcgI 372 CGAGTACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
876 CGACGTGGCTGC
BfaI 676 CTAG C^TAG
1018 CTAG
BlpI 1436 GCTCAGC GC^T[ACGT]AGC
1710 GCTAAGC
Bpu1102I 1436 GCTCAGC GC^T[ACGT]AGC
1710 GCTAAGC
BsaHI 730 GGCGTC G[GA]^CG[CT]C
802 GACGTC
BseAI 572 TCCGGA T^CCGGA
906 TCCGGA
BseRI 1315 GAGGAG GAGGAG(10/8)
1529 GAGGAG
BspEI 572 TCCGGA T^CCGGA
906 TCCGGA
BspMI 812 ACCTGC ACCTGC(4/8)
424 GCAGGT
BsrBI 1434 CCGCTC CCGCTC(-3/-3)
1491 CCGCTC
BstAPI 174 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1474 GCAGTCATTGC
BstEII 708 GGTGACC G^GT[ACGT]ACC
1359 GGTCACC
BstPI 708 GGTGACC G^GT[ACGT]ACC
1359 GGTCACC
BstXI 455 CCATCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
872 CCAGCGACGTGG
CelII 1436 GCTCAGC GC^T[ACGT]AGC
1710 GCTAAGC
DraII 766 GGGGCCC [GA]G^G[ACGT]CC[CT]
1013 GGGACCT
EcoO109I 766 GGGGCCC [GA]G^G[ACGT]CC[CT]
1013 GGGACCT
EcoO65I 708 GGTGACC G^GT[ACGT]ACC
1359 GGTCACC
HgaI 576 GACGC GACGC(5/10)
731 GCGTC
Hin1I 730 GGCGTC G[GA]^CG[CT]C
802 GACGTC
MaeI 676 CTAG C^TAG
1018 CTAG
MroI 572 TCCGGA T^CCGGA
906 TCCGGA
PflMI 144 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
543 CCAGGTGCTGG
PstI 28 CTGCAG CTGCA^G
499 CTGCAG
SfcI 28 CTGCAG C^T[GA][CT]AG
499 CTGCAG
SmaI 784 CCCGGG CCC^GGG
1274 CCCGGG
TaqI 4 TCGA T^CGA
1025 TCGA
Tsp509I 939 AATT ^AATT
1601 AATT
TthHB8I 4 TCGA T^CGA
1025 TCGA
Van91I 144 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
543 CCAGGTGCTGG
XmaCI 784 CCCGGG C^CCGGG
1274 CCCGGG
XmaI 784 CCCGGG C^CCGGG
1274 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI AvaII BanII BbvI
BcnI BglI BmyI BpmI BsaI
BsaJI BsaWI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp45I TspRI
XcmI XhoII