Restriction map for
pFN21AE1475
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BanI BbeI BbrPI BbsI
BbvCI BbvI BcgI BciVI BclI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI MaeIII MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SalI SapI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI SwaI TaqI
Tru9I TseI Tsp45I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AlwNI 475 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 316 CCTCAGG CC^T[ACGT]AGG
AspHI 220 GAGCTC G[AT]GC[AT]^C
AvaII 276 GGACC G^G[AT]CC
BamHI 81 GGATCC G^GATCC
BanII 220 GAGCTC G[GA]GC[CT]^C
BcnI 273 CCGGG CC^[GC]GG
BfaI 229 CTAG C^TAG
BglI 395 GCCGGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 430 GGTCTC GGTCTC(1/5)
BseRI 241 GAGGAG GAGGAG(10/8)
BsiHKAI 220 GAGCTC G[AT]GC[AT]^C
BsmBI 264 GAGACG CGTCTC(1/5)
BsmFI 275 GGGAC GGGAC(10/14)
Bsp1407I 329 TGTACA T^GTACA
BspMI 98 ACCTGC ACCTGC(4/8)
BsrGI 329 TGTACA T^GTACA
BstAPI 474 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 426 CCAGG CC^[AT]GG
BstYI 81 GGATCC [GA]^GATC[CT]
Bsu36I 316 CCTCAGG CC^T[ACGT]AGG
DpnII 82 GATC ^GATC
DsaI 23 CCGCGG C^C[GA][CT]GG
Eco81I 316 CCTCAGG CC^T[ACGT]AGG
EcoRII 426 CCAGG ^CC[AT]GG
EcoT14I 446 CCAAGG C^C[AT][AT]GG
FauI 184 CCCGC CCCGC(4/6)
HinfI 110 GAATC G^A[ACGT]TC
HphI 20 TCACC GGTGA(8/7)
KspI 23 CCGCGG CCGC^GG
MaeI 229 CTAG C^TAG
MaeII 267 ACGT A^CGT
MboI 82 GATC ^GATC
MflI 81 GGATCC [GA]^GATC[CT]
MspA1I 23 CCGCGG C[AC]G^C[GT]G
MvaI 426 CCAGG CC^[AT]GG
NciI 273 CCGGG CC^[GC]GG
NdeII 82 GATC ^GATC
NlaIII 471 CATG CATG^
NspI 470 ACATGC [GA]CATG^[CT]
PpuMI 275 GGGACCC [GA]G^G[AT]CC[CT]
SacI 220 GAGCTC GAGCT^C
SacII 23 CCGCGG CCGC^GG
Sau3AI 82 GATC ^GATC
SbfI 33 CCTGCAGG CCTGCA^GG
Sse8387I 33 CCTGCAGG CCTGCA^GG
SspBI 329 TGTACA T^GTACA
StyI 446 CCAAGG C^C[AT][AT]GG
TfiI 110 GAATC G^A[AT]TC
XhoII 81 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 24 CGCG CG^CG
186 CGCG
AcyI 178 GACGCC G[GA]^CG[CT]C
460 GACGCC
AlwI 82 GATCC GGATC(4/5)
81 GGATC
BmyI 220 GAGCTC G[AGT]GC[ACT]^C
409 GGGCAC
BsaHI 178 GACGCC G[GA]^CG[CT]C
460 GACGCC
BsmAI 264 GAGAC GTCTC(1/5)
431 GTCTC
Bsp1286I 220 GAGCTC G[AGT]GC[ACT]^C
409 GGGCAC
BssKI 273 CCGGG ^CC[ACGT]GG
426 CCAGG
BstUI 24 CGCG CG^CG
186 CGCG
DraII 275 GGGACCC [GA]G^G[ACGT]CC[CT]
476 AGGCCCT
EaeI 26 CGGCCA [CT]^GGCC[GA]
270 TGGCCG
EarI 115 CTCTTC CTCTTC(1/4)
236 GAAGAG
EcoNI 316 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
420 CCTTTACCAGG
EcoO109I 275 GGGACCC [GA]G^G[ACGT]CC[CT]
476 AGGCCCT
Fnu4HI 25 GCGGC GC^[ACGT]GC
297 GCCGC
HaeII 12 GGCGCT [GA]GCGC^[CT]
392 AGCGCC
HapII 273 CCGG C^CGG
396 CCGG
HgaI 178 GACGC GACGC(5/10)
460 GACGC
Hin1I 178 GACGCC G[GA]^CG[CT]C
460 GACGCC
HpaII 273 CCGG C^CGG
396 CCGG
ItaI 25 GCGGC GC^[ACGT]GC
297 GCCGC
Ksp632I 115 CTCTTC CTCTTC(1/4)
236 GAAGAG
MspI 273 CCGG C^CGG
396 CCGG
MvnI 24 CGCG CG^CG
186 CGCG
MwoI 395 GCCGGAGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
474 GCAGGCCCTGC
PstI 34 CTGCAG CTGCA^G
340 CTGCAG
ScrFI 273 CCGGG CC^[ACGT]GG
426 CCAGG
StuI 324 AGGCCT AGG^CCT
362 AGGCCT
TspRI 138 CACTG CA[GC]TG[ACGT][ACGT]^
369 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI AfaI AluI BsaJI BsiYI
BslI BstF5I Cac8I CfoI Cfr13I
DdeI FokI HaeIII HhaI HinP1I
MboII MnlI NlaIV RsaI Sau96I
SfcI