Restriction map for
pFN21AB6231
##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BamHI BbrPI BbvCI BciVI
BfaI BfrI BlnI BsaBI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BspMI BsrDI BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
ClaI CpoI DraIII Eco47III Eco81I
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI KpnI MaeI
MamI MluI MroI NdeI NheI
NsiI NspI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SalI SbfI ScaI
SexAI SfuI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AflIII 1814 ACACGT A^C[GA][CT]GT
AgeI 1651 ACCGGT A^CCGGT
AhdI 1285 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1285 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 270 GAAGAC GAAGAC(2/6)
BcgI 2053 GCAGTGGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 675 TGATCA T^GATCA
BglII 1181 AGATCT A^GATCT
BlpI 1255 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1255 GCTGAGC GC^T[ACGT]AGC
BpuAI 270 GAAGAC GAAGAC(2/6)
BsaI 217 GAGACC GGTCTC(1/5)
Bsp1407I 551 TGTACA T^GTACA
BsrGI 551 TGTACA T^GTACA
BstEII 103 GGTCACC G^GT[ACGT]ACC
BstPI 103 GGTCACC G^GT[ACGT]ACC
BstXI 1673 CCACGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1255 GCTGAGC GC^T[ACGT]AGC
DraI 406 TTTAAA TTT^AAA
DrdI 2011 GACGGCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1285 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1476 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 103 GGTCACC G^GT[ACGT]ACC
EcoRI 829 GAATTC G^AATTC
FbaI 675 TGATCA T^GATCA
MfeI 199 CAATTG C^AATTG
MunI 199 CAATTG C^AATTG
NaeI 1389 GCCGGC GCC^GGC
NgoMIV 1389 GCCGGC G^CCGGC
NruI 1374 TCGCGA TCG^CGA
PflMI 2172 CCAGCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1651 ACCGGT A^CCGGT
SapI 1551 GCTCTTC GCTCTTC(1/4)
SfiI 1516 GGCCTGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1650 CACCGGTG C[GA]^CCGG[CT]G
SspBI 551 TGTACA T^GTACA
TaqI 1087 TCGA T^CGA
TfiI 1669 GATTC G^A[AT]TC
TthHB8I 1087 TCGA T^CGA
Van91I 2172 CCAGCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 490 AAATTT [GA]^AATT[CT]
829 GAATTC
Alw44I 313 GTGCAC G^TGCAC
1867 GTGCAC
AlwNI 1702 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
1768 CAGGGCCTG
ApaLI 313 GTGCAC G^TGCAC
1867 GTGCAC
ApoI 490 AAATTT [GA]^AATT[CT]
829 GAATTC
AspHI 313 GTGCAC G[AT]GC[AT]^C
1867 GTGCAC
BsaAI 684 TACGTG [CT]AC^GT[GA]
1815 CACGTA
BsaWI 21 TCCGGT [AT]^CCGG[AT]
1651 ACCGGT
BsgI 1558 CTGCAC GTGCAG(16/14)
1450 GTGCAG
BsiHKAI 313 GTGCAC G[AT]GC[AT]^C
1867 GTGCAC
BsmAI 217 GAGAC GTCTC(1/5)
1657 GTCTC
BstYI 18 AGATCC [GA]^GATC[CT]
1181 AGATCT
MflI 18 AGATCC [GA]^GATC[CT]
1181 AGATCT
MseI 407 TTAA T^TAA
874 TTAA
PstI 955 CTGCAG CTGCA^G
2161 CTGCAG
Tru9I 407 TTAA T^TAA
874 TTAA
XcmI 182 CCAAGCCGATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
308 CCAAGGTGCACCTGG
XhoII 18 AGATCC [GA]^GATC[CT]
1181 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AvaI AvaII BalI
BanI BanII BbeI BbvI BcnI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsiEI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NotI PleI PpuMI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI StuI
StyI TseI Tsp45I Tsp509I TspRI
XmaCI XmaI