Restriction map for
pFN21AE4361
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# NO CUT ENZYMES ( 137 enzymes) #
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Acc65I AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII BalI BamHI
BbeI BbrPI BcgI BciVI BclI
BfrI BglII BpmI BsaAI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
HinP1I HpaI KasI KpnI KspI
MfeI MflI MluI MluNI MroI
MscI MslI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 52 AAATTC [GA]^AATT[CT]
AfaI 967 GTAC GT^AC
AocI 1060 CCTAAGG CC^T[ACGT]AGG
ApaI 462 GGGCCC GGGCC^C
ApoI 52 AAATTC [GA]^AATT[CT]
Asp700I 48 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 940 GAGCTC G[AT]GC[AT]^C
AvaI 295 CCCGAG C^[CT]CG[GA]G
AvrII 772 CCTAGG C^CTAGG
BanI 523 GGTGCC G^G[CT][GA]CC
BbvCI 533 CCTCAGC CCTCAGC(-5/-2)
BglI 226 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 772 CCTAGG C^CTAGG
BlpI 937 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 937 GCTGAGC GC^T[ACGT]AGC
BsiHKAI 940 GAGCTC G[AT]GC[AT]^C
BsoBI 295 CCCGAG C^[CT]CG[GA]G
Bsp1407I 966 TGTACA T^GTACA
BsrDI 83 CATTGC GCAATG(2/0)
BsrGI 966 TGTACA T^GTACA
Bsu36I 1060 CCTAAGG CC^T[ACGT]AGG
CelII 937 GCTGAGC GC^T[ACGT]AGC
Eco81I 1060 CCTAAGG CC^T[ACGT]AGG
EcoNI 772 CCTAGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 979 GTCAAC GT[CT]^[GA]AC
HindII 979 GTCAAC GT[CT]^[GA]AC
HindIII 492 AAGCTT A^AGCTT
MaeIII 898 GTCAC ^GT[ACGT]AC
MseI 286 TTAA T^TAA
MspA1I 199 CAGCTG C[AC]G^C[GT]G
NcoI 869 CCATGG C^CATGG
PflMI 466 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 43 CTGCAG CTGCA^G
PvuII 199 CAGCTG CAG^CTG
RsaI 967 GTAC GT^AC
SacI 940 GAGCTC GAGCT^C
SfaNI 1009 GATGC GCATC(5/9)
SspBI 966 TGTACA T^GTACA
TfiI 748 GATTC G^A[AT]TC
Tru9I 286 TTAA T^TAA
Tsp45I 898 GTCAC ^GT[GC]AC
Tsp509I 53 AATT ^AATT
Van91I 466 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 48 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AatII 374 GACGTC GACGT^C
1108 GACGTC
AccI 1096 GTCTAC GT^[AC][GT]AC
1111 GTCTAC
AcyI 374 GACGTC G[GA]^CG[CT]C
1108 GACGTC
AlwNI 467 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
882 CAGAAACTG
BbsI 805 GAAGAC GAAGAC(2/6)
411 GTCTTC
BpuAI 805 GAAGAC GAAGAC(2/6)
411 GTCTTC
BsaHI 374 GACGTC G[GA]^CG[CT]C
1108 GACGTC
BspMI 356 ACCTGC ACCTGC(4/8)
740 GCAGGT
BstNI 466 CCAGG CC^[AT]GG
954 CCAGG
DsaI 828 CCGTGG C^C[GA][CT]GG
869 CCATGG
EarI 31 GAAGAG CTCTTC(1/4)
451 GAAGAG
EcoRII 466 CCAGG ^CC[AT]GG
954 CCAGG
Hin1I 374 GACGTC G[GA]^CG[CT]C
1108 GACGTC
Ksp632I 31 GAAGAG CTCTTC(1/4)
451 GAAGAG
MaeII 375 ACGT A^CGT
1109 ACGT
MvaI 466 CCAGG CC^[AT]GG
954 CCAGG
SfcI 43 CTGCAG C^T[GA][CT]AG
1098 CTACAG
SmlI 64 CTCAAG C^T[CT][GA]AG
274 CTTGAG
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BcnI BfaI BmyI BsaBI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrBI BsrI
BssKI BstF5I Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MamI
MboI MboII MnlI MspI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI Sau3AI Sau96I ScrFI StyI
TaqI TseI TspRI TthHB8I