Restriction map for
pFN21AE6308
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AhdI AocI ApaI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BamHI
BbrPI BcgI BciVI BfrI BlnI
BlpI Bpu1102I BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoRI EcoRV EcoT14I EcoT22I
FseI FspI HincII HindII HindIII
HpaI KspI MfeI MluI MroI
MseI MspA1I MunI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tru9I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 596 GGTACC G^GTACC
AccII 96 CGCG CG^CG
AcyI 340 GGCGCC G[GA]^CG[CT]C
Alw44I 649 GTGCAC G^TGCAC
Aor51HI 219 AGCGCT AGC^GCT
ApaLI 649 GTGCAC G^TGCAC
Asp718I 596 GGTACC G^GTACC
AspHI 649 GTGCAC G[AT]GC[AT]^C
AvaI 161 CCCGGG C^[CT]CG[GA]G
BalI 686 TGGCCA TGG^CCA
BanII 358 GAGCCC G[GA]GC[CT]^C
BbeI 340 GGCGCC GGCGC^C
BbvCI 215 CCTCAGC CCTCAGC(-5/-2)
BclI 567 TGATCA T^GATCA
BfaI 133 CTAG C^TAG
BglI 85 GCCAAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1001 AGATCT A^GATCT
BsaAI 879 CACGTA [CT]AC^GT[GA]
BsaBI 711 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 340 GGCGCC G[GA]^CG[CT]C
BsiHKAI 649 GTGCAC G[AT]GC[AT]^C
BsoBI 161 CCCGGG C^[CT]CG[GA]G
BsrDI 285 CATTGC GCAATG(2/0)
BstAPI 1122 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1135 GGTAACC G^GT[ACGT]ACC
BstPI 1135 GGTAACC G^GT[ACGT]ACC
BstUI 96 CGCG CG^CG
DsaI 632 CCGTGG C^C[GA][CT]GG
Eco47III 219 AGCGCT AGC^GCT
EcoNI 31 CCTGAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1135 GGTAACC G^GT[ACGT]ACC
FbaI 567 TGATCA T^GATCA
HgaI 97 GCGTC GACGC(5/10)
Hin1I 340 GGCGCC G[GA]^CG[CT]C
KasI 340 GGCGCC G^GCGCC
KpnI 596 GGTACC GGTAC^C
MaeI 133 CTAG C^TAG
MamI 711 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 686 TGGCCA TGG^CCA
MscI 686 TGGCCA TGG^CCA
MvnI 96 CGCG CG^CG
NaeI 1081 GCCGGC GCC^GGC
NarI 340 GGCGCC GG^CGCC
NgoMIV 1081 GCCGGC G^CCGGC
PleI 700 GAGTC GAGTC(4/5)
SapI 1035 GCTCTTC GCTCTTC(1/4)
SexAI 254 ACCTGGT A^CC[AT]GGT
SfoI 340 GGCGCC GGC^GCC
SmaI 161 CCCGGG CCC^GGG
StuI 852 AGGCCT AGG^CCT
Tsp509I 464 AATT ^AATT
XmaCI 161 CCCGGG C^CCGGG
XmaI 161 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AfaI 597 GTAC GT^AC
882 GTAC
AgeI 154 ACCGGT A^CCGGT
1014 ACCGGT
AlwI 674 GATCC GGATC(4/5)
753 GATCC
AlwNI 728 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1123 CAGAACCTG
BbsI 814 GAAGAC GAAGAC(2/6)
909 GAAGAC
BbvI 110 GCAGC GCAGC(8/12)
405 GCTGC
BpuAI 814 GAAGAC GAAGAC(2/6)
909 GAAGAC
BsaI 151 GAGACC GGTCTC(1/5)
1153 GAGACC
BsaWI 154 ACCGGT [AT]^CCGG[AT]
1014 ACCGGT
BsgI 319 GTGCAG GTGCAG(16/14)
1018 GTGCAG
BsmAI 151 GAGAC GTCTC(1/5)
1153 GAGAC
BsmFI 419 GGGAC GGGAC(10/14)
747 GTCCC
BstF5I 1050 CATCC GGATG(2/0)
636 GGATG
BstYI 752 AGATCC [GA]^GATC[CT]
1001 AGATCT
DdeI 32 CTGAG C^T[ACGT]AG
216 CTCAG
DraII 143 GGGGCCT [GA]G^G[ACGT]CC[CT]
482 AGGCCCC
EarI 1036 CTCTTC CTCTTC(1/4)
697 GAAGAG
EcoO109I 143 GGGGCCT [GA]G^G[ACGT]CC[CT]
482 AGGCCCC
FokI 1050 CATCC GGATG(9/13)
636 GGATG
Ksp632I 1036 CTCTTC CTCTTC(1/4)
697 GAAGAG
MaeII 412 ACGT A^CGT
880 ACGT
MflI 752 AGATCC [GA]^GATC[CT]
1001 AGATCT
PflMI 546 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
727 CCAGCGCCTGG
PinAI 154 ACCGGT A^CCGGT
1014 ACCGGT
RsaI 597 GTAC GT^AC
882 GTAC
SfaNI 711 GATGC GCATC(5/9)
943 GATGC
SmlI 805 CTCAAG C^T[CT][GA]AG
840 CTTGAG
TfiI 44 GATTC G^A[AT]TC
831 GATTC
TseI 110 GCAGC G^C[AT]GC
405 GCTGC
TspRI 12 CAGTG CA[GC]TG[ACGT][ACGT]^
1097 CACTG
Van91I 546 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
727 CCAGCGCCTGG
XhoII 752 AGATCC [GA]^GATC[CT]
1001 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AvaII BanI BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI Bsp1286I BspMI BsrFI BssKI
BstNI BstXI Cac8I CfoI Cfr10I
Cfr13I DpnII DraIII EaeI EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PstI Sau3AI
Sau96I ScrFI SfcI TaqI Tsp45I
TthHB8I XcmI