Restriction map for
pFN21AE3634
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AvaII AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BbvI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
Cfr13I ClaI CpoI DdeI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
Fnu4HI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI ItaI
KasI KspI MaeI MaeII MaeIII
MfeI MflI MluI MroI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI Sau96I
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI TseI
Tsp45I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 78 GGTACC G^GTACC
AciI 160 CCGC CCGC(-3/-1)
AfaI 79 GTAC GT^AC
AluI 290 AGCT AG^CT
Aor51HI 308 AGCGCT AGC^GCT
Asp718I 78 GGTACC G^GTACC
AspHI 202 GAGCAC G[AT]GC[AT]^C
BalI 320 TGGCCA TGG^CCA
BanI 78 GGTACC G^G[CT][GA]CC
BbsI 112 GAAGAC GAAGAC(2/6)
BmyI 202 GAGCAC G[AGT]GC[ACT]^C
BpuAI 112 GAAGAC GAAGAC(2/6)
BsaBI 210 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 304 GAGGAG GAGGAG(10/8)
BsgI 223 GTGCAG GTGCAG(16/14)
BsiHKAI 202 GAGCAC G[AT]GC[AT]^C
Bsp1286I 202 GAGCAC G[AGT]GC[ACT]^C
BsrI 123 ACTGG ACTGG(1/-1)
BssKI 249 CCAGG ^CC[ACGT]GG
BstF5I 216 CATCC GGATG(2/0)
BstNI 249 CCAGG CC^[AT]GG
Cac8I 175 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 309 GCGC GCG^C
EaeI 320 TGGCCA [CT]^GGCC[GA]
EarI 34 CTCTTC CTCTTC(1/4)
Eco47III 308 AGCGCT AGC^GCT
EcoNI 297 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 249 CCAGG ^CC[AT]GG
FauI 159 CCCGC CCCGC(4/6)
FokI 216 CATCC GGATG(9/13)
HaeII 308 AGCGCT [GA]GCGC^[CT]
HaeIII 321 GGCC GG^CC
HapII 235 CCGG C^CGG
HhaI 309 GCGC GCG^C
HinP1I 309 GCGC G^CGC
HinfI 22 GAGTC G^A[ACGT]TC
HpaII 235 CCGG C^CGG
KpnI 78 GGTACC GGTAC^C
Ksp632I 34 CTCTTC CTCTTC(1/4)
MamI 210 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 320 TGGCCA TGG^CCA
MscI 320 TGGCCA TGG^CCA
MslI 216 CATCCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 235 CCGG C^CGG
MvaI 249 CCAGG CC^[AT]GG
NlaIV 78 GGTACC GG[ACGT]^[ACGT]CC
PleI 22 GAGTC GAGTC(4/5)
RsaI 79 GTAC GT^AC
SapI 33 GCTCTTC GCTCTTC(1/4)
ScrFI 249 CCAGG CC^[ACGT]GG
SfcI 240 CTACAG C^T[GA][CT]AG
Tsp509I 345 AATT ^AATT
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# TWO CUT ENZYMES ( 15 enzymes) #
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BpmI 11 CTCCAG CTGGAG(16/14)
301 CTGGAG
BsmFI 187 GTCCC GGGAC(10/14)
294 GTCCC
DpnII 54 GATC ^GATC
210 GATC
EcoT14I 74 CCAAGG C^C[AT][AT]GG
197 CCTTGG
MboI 54 GATC ^GATC
210 GATC
MseI 62 TTAA T^TAA
137 TTAA
NdeII 54 GATC ^GATC
210 GATC
PflMI 117 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
192 CCACCCCTTGG
Sau3AI 54 GATC ^GATC
210 GATC
StyI 74 CCAAGG C^C[AT][AT]GG
197 CCTTGG
TaqI 20 TCGA T^CGA
38 TCGA
Tru9I 62 TTAA T^TAA
137 TTAA
TspRI 122 CACTG CA[GC]TG[ACGT][ACGT]^
285 CAGTG
TthHB8I 20 TCGA T^CGA
38 TCGA
Van91I 117 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
192 CCACCCCTTGG
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# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
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BsaJI BsiYI BslI HphI MboII
MnlI MwoI