Restriction map for
pFN21AE3936
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# NO CUT ENZYMES ( 191 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HaeIII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MslI MunI MvnI MwoI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tsp45I TspRI
Tth111I XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AlwI 444 GGATC GGATC(4/5)
AlwNI 401 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 118 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 473 GTGCTC G[AT]GC[AT]^C
BcnI 521 CCCGG CC^[GC]GG
BmyI 473 GTGCTC G[AGT]GC[ACT]^C
BsiHKAI 473 GTGCTC G[AT]GC[AT]^C
BsmAI 213 GTCTC GTCTC(1/5)
BsmFI 66 GGGAC GGGAC(10/14)
Bsp1286I 473 GTGCTC G[AGT]GC[ACT]^C
BspHI 333 TCATGA T^CATGA
BsrDI 149 GCAATG GCAATG(2/0)
BsrI 69 ACTGG ACTGG(1/-1)
BstNI 227 CCTGG CC^[AT]GG
DdeI 430 CTTAG C^T[ACGT]AG
DsaI 451 CCATGG C^C[GA][CT]GG
EarI 31 CTCTTC CTCTTC(1/4)
EcoRII 227 CCTGG ^CC[AT]GG
HapII 522 CCGG C^CGG
HinfI 211 GAGTC G^A[ACGT]TC
HpaII 522 CCGG C^CGG
HphI 425 TCACC GGTGA(8/7)
Ksp632I 31 CTCTTC CTCTTC(1/4)
MaeIII 38 GTAAC ^GT[ACGT]AC
MspA1I 404 CAGCTG C[AC]G^C[GT]G
MspI 522 CCGG C^CGG
MvaI 227 CCTGG CC^[AT]GG
NciI 521 CCCGG CC^[GC]GG
NcoI 451 CCATGG C^CATGG
NlaIV 26 GGAGCC GG[ACGT]^[ACGT]CC
PflMI 17 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 211 GAGTC GAGTC(4/5)
PstI 353 CTGCAG CTGCA^G
PvuII 404 CAGCTG CAG^CTG
RcaI 333 TCATGA T^CATGA
SfcI 353 CTGCAG C^T[GA][CT]AG
TaqI 415 TCGA T^CGA
TthHB8I 415 TCGA T^CGA
Van91I 17 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 504 TCTAGA T^CTAGA
XmnI 118 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AluI 297 AGCT AG^CT
405 AGCT
BbvI 403 GCAGC GCAGC(8/12)
352 GCTGC
BfaI 295 CTAG C^TAG
505 CTAG
BsaJI 451 CCATGG C^C[ACGT][ACGT]GG
498 CCAAGG
BsiYI 17 CCAAACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
136 CCAACCATAGG
BslI 17 CCAAACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
136 CCAACCATAGG
BssKI 227 CCTGG ^CC[ACGT]GG
521 CCCGG
EcoT14I 451 CCATGG C^C[AT][AT]GG
498 CCAAGG
Fnu4HI 352 GCTGC GC^[ACGT]GC
403 GCAGC
ItaI 352 GCTGC GC^[ACGT]GC
403 GCAGC
MaeI 295 CTAG C^TAG
505 CTAG
MseI 109 TTAA T^TAA
440 TTAA
NlaIII 334 CATG CATG^
452 CATG
ScrFI 227 CCTGG CC^[ACGT]GG
521 CCCGG
StyI 451 CCATGG C^C[AT][AT]GG
498 CCAAGG
Tru9I 109 TTAA T^TAA
440 TTAA
TseI 352 GCTGC G^C[AT]GC
403 GCAGC
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
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BstF5I DpnII FokI MboI MboII
MnlI NdeII Sau3AI Tsp509I