Restriction map for
pFN21AE4343
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AclI AflII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BfaI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BseRI BsiWI
BsmBI Bsp1407I BspDI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Van91I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 853 GTATAC GT^[AC][GT]AC
AccII 21 CGCG CG^CG
AccIII 633 TCCGGA T^CCGGA
AcsI 731 AAATTT [GA]^AATT[CT]
AflIII 573 ACATGT A^C[GA][CT]GT
AlwNI 745 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 731 AAATTT [GA]^AATT[CT]
AspI 896 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 276 TGGCCA TGG^CCA
BanII 528 GGGCTC G[GA]GC[CT]^C
BclI 809 TGATCA T^GATCA
BglII 554 AGATCT A^GATCT
BsaWI 633 TCCGGA [AT]^CCGG[AT]
BseAI 633 TCCGGA T^CCGGA
BsiEI 26 CGGCCG CG[GA][CT]^CG
BspEI 633 TCCGGA T^CCGGA
BspLU11I 573 ACATGT A^CATGT
Bst1107I 853 GTATAC GTA^TAC
BstUI 21 CGCG CG^CG
BstXI 1034 CCACCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 554 AGATCT [GA]^GATC[CT]
BstZ17I 853 GTATAC GTA^TAC
CfoI 9 GCGC GCG^C
EagI 26 CGGCCG C^GGCCG
EclXI 26 CGGCCG C^GGCCG
Eco52I 26 CGGCCG C^GGCCG
EcoT14I 228 CCAAGG C^C[AT][AT]GG
FauI 381 CCCGC CCCGC(4/6)
FbaI 809 TGATCA T^GATCA
HhaI 9 GCGC GCG^C
HinP1I 9 GCGC G^CGC
MflI 554 AGATCT [GA]^GATC[CT]
MluNI 276 TGGCCA TGG^CCA
MroI 633 TCCGGA T^CCGGA
MscI 276 TGGCCA TGG^CCA
MseI 49 TTAA T^TAA
MvnI 21 CGCG CG^CG
PaeR7I 220 CTCGAG C^TCGAG
PflFI 896 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 667 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 160 CTGCAG CTGCA^G
SapI 622 GAAGAGC GCTCTTC(1/4)
SmaI 606 CCCGGG CCC^GGG
SmlI 220 CTCGAG C^T[CT][GA]AG
SspI 650 AATATT AAT^ATT
StuI 495 AGGCCT AGG^CCT
StyI 228 CCAAGG C^C[AT][AT]GG
TfiI 611 GATTC G^A[AT]TC
Tru9I 49 TTAA T^TAA
Tsp509I 732 AATT ^AATT
Tth111I 896 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 220 CTCGAG C^TCGAG
XhoII 554 AGATCT [GA]^GATC[CT]
XmaCI 606 CCCGGG C^CCGGG
XmaI 606 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 202 GACGCC G[GA]^CG[CT]C
466 GACGCC
AvaI 220 CTCGAG C^[CT]CG[GA]G
606 CCCGGG
BpmI 193 CTCCAG CTGGAG(16/14)
325 CTCCAG
BsaHI 202 GACGCC G[GA]^CG[CT]C
466 GACGCC
BsmI 598 GCATTC GAATGC(1/-1)
935 GCATTC
BsoBI 220 CTCGAG C^[CT]CG[GA]G
606 CCCGGG
EaeI 26 CGGCCG [CT]^GGCC[GA]
276 TGGCCA
EarI 622 GAAGAG CTCTTC(1/4)
987 GAAGAG
HgaI 202 GACGC GACGC(5/10)
466 GACGC
Hin1I 202 GACGCC G[GA]^CG[CT]C
466 GACGCC
Ksp632I 622 GAAGAG CTCTTC(1/4)
987 GAAGAG
MaeIII 185 GTGAC ^GT[ACGT]AC
563 GTCAC
NspI 308 ACATGC [GA]CATG^[CT]
573 ACATGT
PleI 900 GAGTC GAGTC(4/5)
1016 GAGTC
PvuII 349 CAGCTG CAG^CTG
745 CAGCTG
SfaNI 908 GCATC GCATC(5/9)
944 GCATC
SfcI 160 CTGCAG C^T[GA][CT]AG
336 CTACAG
TaqI 221 TCGA T^CGA
839 TCGA
Tsp45I 185 GTGAC ^GT[GC]AC
563 GTCAC
TthHB8I 221 TCGA T^CGA
839 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BbvI BcnI BmyI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI TseI TspRI