Restriction map for
pFN21AE5118
##################################
# NO CUT ENZYMES ( 157 enzymes) #
##################################
Acc65I AccIII AciI AclI AflII
AflIII AgeI AhdI Alw44I AlwI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BstAPI BstBI
BstF5I Cac8I CelII Cfr10I ClaI
CpoI DraI DraIII DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FokI FseI FspI HaeII
HgaI HindIII HinfI HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MseI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I TspRI Tth111I TthHB8I Van91I
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 313 GACGTC GACGT^C
AccI 413 GTATAC GT^[AC][GT]AC
AccII 310 CGCG CG^CG
AcsI 324 AAATTT [GA]^AATT[CT]
AcyI 313 GACGTC G[GA]^CG[CT]C
AfaI 81 GTAC GT^AC
AluI 182 AGCT AG^CT
AocI 402 CCTCAGG CC^T[ACGT]AGG
ApoI 324 AAATTT [GA]^AATT[CT]
Asp700I 257 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 278 CCCGGG C^[CT]CG[GA]G
BbsI 58 GTCTTC GAAGAC(2/6)
BfaI 347 CTAG C^TAG
BglII 389 AGATCT A^GATCT
BpuAI 58 GTCTTC GAAGAC(2/6)
BsaHI 313 GACGTC G[GA]^CG[CT]C
BsmFI 428 GTCCC GGGAC(10/14)
BsoBI 278 CCCGGG C^[CT]CG[GA]G
BspHI 329 TCATGA T^CATGA
BspMI 104 ACCTGC ACCTGC(4/8)
BssSI 364 CTCGTG CACGAG(-5/-1)
Bst1107I 413 GTATAC GTA^TAC
BstEII 157 GGTGACC G^GT[ACGT]ACC
BstPI 157 GGTGACC G^GT[ACGT]ACC
BstUI 310 CGCG CG^CG
BstXI 32 CCATGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 389 AGATCT [GA]^GATC[CT]
BstZ17I 413 GTATAC GTA^TAC
Bsu36I 402 CCTCAGG CC^T[ACGT]AGG
CfoI 309 GCGC GCG^C
DpnII 390 GATC ^GATC
DrdI 291 GACGGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 368 TGGCCG [CT]^GGCC[GA]
Eco81I 402 CCTCAGG CC^T[ACGT]AGG
EcoO65I 157 GGTGACC G^GT[ACGT]ACC
HhaI 309 GCGC GCG^C
Hin1I 313 GACGTC G[GA]^CG[CT]C
HinP1I 309 GCGC G^CGC
HincII 165 GTTGAC GT[CT]^[GA]AC
HindII 165 GTTGAC GT[CT]^[GA]AC
HphI 157 GGTGA GGTGA(8/7)
MaeI 347 CTAG C^TAG
MaeII 314 ACGT A^CGT
MboI 390 GATC ^GATC
MflI 389 AGATCT [GA]^GATC[CT]
MslI 103 CACCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 310 CGCG CG^CG
NdeII 390 GATC ^GATC
PshAI 210 GACGATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 318 CTGCAG CTGCA^G
RcaI 329 TCATGA T^CATGA
RsaI 81 GTAC GT^AC
Sau3AI 390 GATC ^GATC
ScaI 80 AGTACT AGT^ACT
SmaI 278 CCCGGG CCC^GGG
Tsp509I 325 AATT ^AATT
XbaI 346 TCTAGA T^CTAGA
XhoII 389 AGATCT [GA]^GATC[CT]
XmaCI 278 CCCGGG C^CCGGG
XmaI 278 CCCGGG C^CCGGG
XmnI 257 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwNI 178 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
198 CAGGACCTG
ApaI 11 GGGCCC GGGCC^C
147 GGGCCC
BbvI 28 GCAGC GCAGC(8/12)
271 GCTGC
BcnI 278 CCCGG CC^[GC]GG
279 CCGGG
BseRI 115 GAGGAG GAGGAG(10/8)
333 GAGGAG
EcoNI 135 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
418 CCTTCTACAGG
Fnu4HI 28 GCAGC GC^[ACGT]GC
271 GCTGC
HapII 279 CCGG C^CGG
371 CCGG
HpaII 279 CCGG C^CGG
371 CCGG
ItaI 28 GCAGC GC^[ACGT]GC
271 GCTGC
MspI 279 CCGG C^CGG
371 CCGG
MwoI 4 GCTACCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
311 GCGACGTCTGC
NciI 278 CCCGG CC^[GC]GG
279 CCGGG
NlaIII 33 CATG CATG^
330 CATG
TseI 28 GCAGC G^C[AT]GC
271 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AvaII BanII BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstNI Cfr13I
DdeI DraII EcoO109I EcoRII HaeIII
MaeIII MboII MnlI MvaI NlaIV
PpuMI Sau96I ScrFI SfcI Tsp45I