##################################
# NO CUT ENZYMES (  223 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DdeI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspA1I      MspI        MunI        
MvaI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        Tru9I       Tsp509I     TspRI       
Tth111I     TthHB8I     XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AccI          301  GTAGAC               GT^[AC][GT]AC
AfaI          297  GTAC                 GT^AC
AlwI          148  GGATC                GGATC(4/5)
BanI          232  GGCACC               G^G[CT][GA]CC
BbvI           64  GCTGC                GCAGC(8/12)
BfaI          247  CTAG                 C^TAG
BsaAI         298  TACGTA               [CT]AC^GT[GA]
BsrI          184  CCAGT                ACTGG(1/-1)
Cac8I         126  GCAAGC               GC[ACGT]^[ACGT]GC
DpnII         149  GATC                 ^GATC
Fnu4HI         64  GCTGC                GC^[ACGT]GC
HinfI         177  GATTC                G^A[ACGT]TC
HphI          280  GGTGA                GGTGA(8/7)
ItaI           64  GCTGC                GC^[ACGT]GC
MaeI          247  CTAG                 C^TAG
MaeII         299  ACGT                 A^CGT
MboI          149  GATC                 ^GATC
MwoI            4  GCAGGACAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         149  GATC                 ^GATC
NlaIV         232  GGCACC               GG[ACGT]^[ACGT]CC
PflMI         220  CCAGAATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI          297  GTAC                 GT^AC
Sau3AI        149  GATC                 ^GATC
SmlI          110  CTCAAG               C^T[CT][GA]AG
SnaBI         298  TACGTA               TAC^GTA
TfiI          177  GATTC                G^A[AT]TC
TseI           64  GCTGC                G^C[AT]GC
Tsp45I        281  GTGAC                ^GT[GC]AC
Van91I        220  CCAGAATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          246  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (    5 enzymes) #
###################################

BsiYI          42  CCGAGTATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              220  CCAGAATATGG         
BslI           42  CCGAGTATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              220  CCAGAATATGG         
MaeIII         76  GTAAC                ^GT[ACGT]AC
              281  GTGAC               
MboII          27  TCTTC                GAAGA(8/7)
              213  TCTTC               
MnlI           86  GAGG                 CCTC(7/6)
              230  GAGG                


#########################################
# 3 OR MORE CUT ENZYMES (    0 enzymes) #
#########################################