Restriction map for
pFN21AE1317
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# NO CUT ENZYMES ( 98 enzymes) #
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Acc65I AccI AclI AflII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BanI BbeI BcgI BciVI
BfrI BlnI BsiEI BsiWI BsmBI
BsmI BspHI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I Bsu36I CfoI Cfr10I CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRV EcoT22I FauI FseI
FspI HaeII HgaI HhaI HinP1I
HindIII HpaI KasI KpnI KspI
MluI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AatII 1411 GACGTC GACGT^C
AcyI 1411 GACGTC G[GA]^CG[CT]C
AflIII 905 ACACGT A^C[GA][CT]GT
Alw44I 765 GTGCAC G^TGCAC
AlwNI 214 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 14 GGGCCC GGGCC^C
ApaLI 765 GTGCAC G^TGCAC
AspI 1999 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 548 CTCGAG C^[CT]CG[GA]G
BalI 878 TGGCCA TGG^CCA
BamHI 638 GGATCC G^GATCC
BbrPI 906 CACGTG CAC^GTG
BbsI 1408 GAAGAC GAAGAC(2/6)
BcnI 1494 CCCGG CC^[GC]GG
BglII 2106 AGATCT A^GATCT
BlpI 748 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 748 GCTCAGC GC^T[ACGT]AGC
BpuAI 1408 GAAGAC GAAGAC(2/6)
BsaAI 906 CACGTG [CT]AC^GT[GA]
BsaBI 1512 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1411 GACGTC G[GA]^CG[CT]C
BsaI 551 GAGACC GGTCTC(1/5)
BseRI 255 CTCCTC GAGGAG(10/8)
BsoBI 548 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2135 TGTACA T^GTACA
BspDI 1296 ATCGAT AT^CGAT
BspMI 1162 GCAGGT ACCTGC(4/8)
BsrGI 2135 TGTACA T^GTACA
BstAPI 210 GCACCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 678 GGTCACC G^GT[ACGT]ACC
BstPI 678 GGTCACC G^GT[ACGT]ACC
CelII 748 GCTCAGC GC^T[ACGT]AGC
ClaI 1296 ATCGAT AT^CGAT
DraI 1332 TTTAAA TTT^AAA
DraII 473 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 27 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1171 GACAAAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 786 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 473 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 678 GGTCACC G^GT[ACGT]ACC
Hin1I 1411 GACGTC G[GA]^CG[CT]C
MamI 1512 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 491 CAATTG C^AATTG
MluNI 878 TGGCCA TGG^CCA
MscI 878 TGGCCA TGG^CCA
MspA1I 2090 CAGCTG C[AC]G^C[GT]G
MunI 491 CAATTG C^AATTG
NciI 1494 CCCGG CC^[GC]GG
NdeI 643 CATATG CA^TATG
PaeR7I 548 CTCGAG C^TCGAG
PflFI 1999 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 906 CACGTG CAC^GTG
PmlI 906 CACGTG CAC^GTG
PvuII 2090 CAGCTG CAG^CTG
SacI 746 GAGCTC GAGCT^C
SexAI 368 ACCAGGT A^CC[AT]GGT
SfiI 15 GGCCCAAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 2135 TGTACA T^GTACA
Tth111I 1999 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2185 TCTAGA T^CTAGA
XhoI 548 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccII 174 CGCG CG^CG
598 CGCG
AccIII 296 TCCGGA T^CCGGA
2147 TCCGGA
BanII 14 GGGCCC G[GA]GC[CT]^C
746 GAGCTC
BbvCI 205 CCTCAGC CCTCAGC(-5/-2)
2087 CCTCAGC
BglI 16 GCCCAAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1654 GCCACCCAGGC
BseAI 296 TCCGGA T^CCGGA
2147 TCCGGA
BspEI 296 TCCGGA T^CCGGA
2147 TCCGGA
BstUI 174 CGCG CG^CG
598 CGCG
Cac8I 122 GCCTGC GC[ACGT]^[ACGT]GC
1917 GCCAGC
EaeI 878 TGGCCA [CT]^GGCC[GA]
1856 CGGCCA
EarI 117 GAAGAG CTCTTC(1/4)
264 GAAGAG
EcoRI 543 GAATTC G^AATTC
574 GAATTC
Ksp632I 117 GAAGAG CTCTTC(1/4)
264 GAAGAG
MroI 296 TCCGGA T^CCGGA
2147 TCCGGA
MvnI 174 CGCG CG^CG
598 CGCG
NcoI 881 CCATGG C^CATGG
1792 CCATGG
PflMI 26 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
369 CCAGGTGTTGG
SapI 117 GAAGAGC GCTCTTC(1/4)
264 GAAGAGC
Van91I 26 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
369 CCAGGTGTTGG
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbvI BclI
BfaI BmyI BpmI BsaJI BsaWI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII DsaI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII