Restriction map for
pFN21AE1554
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI Asp700I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeIII HgaI Hin1I HincII HindII
HpaI KasI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 721 GGTACC G^GTACC
AfaI 722 GTAC GT^AC
AlwNI 376 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 544 ATTAAT AT^TAAT
AsnI 544 ATTAAT AT^TAAT
Asp718I 721 GGTACC G^GTACC
AspHI 812 GAGCAC G[AT]GC[AT]^C
AvaI 477 CTCGGG C^[CT]CG[GA]G
BanII 480 GGGCTC G[GA]GC[CT]^C
BbsI 77 GTCTTC GAAGAC(2/6)
BcnI 260 CCCGG CC^[GC]GG
BfaI 124 CTAG C^TAG
BglII 251 AGATCT A^GATCT
BpmI 241 CTGGAG CTGGAG(16/14)
BpuAI 77 GTCTTC GAAGAC(2/6)
BseRI 743 GAGGAG GAGGAG(10/8)
BsiHKAI 812 GAGCAC G[AT]GC[AT]^C
BsmFI 246 GGGAC GGGAC(10/14)
BsoBI 477 CTCGGG C^[CT]CG[GA]G
BstXI 640 CCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 695 GCGC GCG^C
DraII 128 GGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 402 GAAGAG CTCTTC(1/4)
EcoO109I 128 GGGTCCT [GA]G^G[ACGT]CC[CT]
HaeII 694 GGCGCT [GA]GCGC^[CT]
HapII 261 CCGG C^CGG
HhaI 695 GCGC GCG^C
HinP1I 695 GCGC G^CGC
HindIII 278 AAGCTT A^AGCTT
HpaII 261 CCGG C^CGG
KpnI 721 GGTACC GGTAC^C
Ksp632I 402 GAAGAG CTCTTC(1/4)
MaeI 124 CTAG C^TAG
MspA1I 554 CAGCTG C[AC]G^C[GT]G
MspI 261 CCGG C^CGG
NciI 260 CCCGG CC^[GC]GG
NspI 170 GCATGC [GA]CATG^[CT]
PpuMI 128 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 544 ATTAAT AT^TAAT
PvuII 554 CAGCTG CAG^CTG
RsaI 722 GTAC GT^AC
SmlI 304 CTCAAG C^T[CT][GA]AG
SphI 170 GCATGC GCATG^C
Tsp45I 151 GTGAC ^GT[GC]AC
TspRI 398 CACTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 433 CCGC CCGC(-3/-1)
757 GCGG
AlwI 21 GATCC GGATC(4/5)
443 GGATC
AvaII 36 GGACC G^G[AT]CC
129 GGTCC
BanI 104 GGTGCC G^G[CT][GA]CC
721 GGTACC
BmyI 480 GGGCTC G[AGT]GC[ACT]^C
812 GAGCAC
BsiYI 132 CCTTTGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
323 CCCTTGCTCGG
BslI 132 CCTTTGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
323 CCCTTGCTCGG
BsmAI 618 GTCTC GTCTC(1/5)
700 GTCTC
Bsp1286I 480 GGGCTC G[AGT]GC[ACT]^C
812 GAGCAC
Cfr13I 36 GGACC G^G[ACGT]CC
129 GGTCC
DdeI 255 CTCAG C^T[ACGT]AG
808 CTAAG
DsaI 97 CCATGG C^C[GA][CT]GG
640 CCATGG
EcoT14I 97 CCATGG C^C[AT][AT]GG
640 CCATGG
HphI 225 TCACC GGTGA(8/7)
369 TCACC
MaeIII 151 GTGAC ^GT[ACGT]AC
464 GTTAC
MseI 488 TTAA T^TAA
545 TTAA
MslI 98 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
156 CATTATGGTG
NcoI 97 CCATGG C^CATGG
640 CCATGG
Sau96I 36 GGACC G^G[ACGT]CC
129 GGTCC
SfaNI 526 GATGC GCATC(5/9)
669 GATGC
StyI 97 CCATGG C^C[AT][AT]GG
640 CCATGG
TfiI 378 GAATC G^A[AT]TC
384 GAATC
Tru9I 488 TTAA T^TAA
545 TTAA
XcmI 155 CCATTATGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
350 CCAAAGAGCAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AluI BbvI BsaJI BsrI BssKI
BstF5I BstNI BstYI Cac8I DpnII
EcoRII Fnu4HI FokI HinfI ItaI
MboI MboII MflI MnlI MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI ScrFI TseI Tsp509I XhoII