Restriction map for
pFN21AE6150
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvaI
AvrII BamHI BanII BbsI BcgI
BciVI BclI BcnI BfaI BfrI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI HincII HindII
HindIII HinfI HpaI HphI KpnI
KspI MaeI MaeIII MamI MfeI
MluI MroI MseI MspA1I MunI
MvnI NciI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tru9I Tsp45I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 370 GGCGCC G[GA]^CG[CT]C
AspI 379 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 329 GGACC G^G[AT]CC
BalI 341 TGGCCA TGG^CCA
BbeI 370 GGCGCC GGCGC^C
BbrPI 121 CACGTG CAC^GTG
BbvCI 84 GCTGAGG CCTCAGC(-5/-2)
BglI 97 GCCGTAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 121 CACGTG [CT]AC^GT[GA]
BsaHI 370 GGCGCC G[GA]^CG[CT]C
BsaI 223 GAGACC GGTCTC(1/5)
BsmAI 223 GAGAC GTCTC(1/5)
BsmFI 308 GGGAC GGGAC(10/14)
BsrFI 13 GCCGGC [GA]^CCGG[CT]
BstF5I 164 CATCC GGATG(2/0)
BstXI 332 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 13 GCCGGC [GA]^CCGG[CT]
DraIII 65 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 296 CTCTTC CTCTTC(1/4)
FokI 164 CATCC GGATG(9/13)
HaeII 370 GGCGCC [GA]GCGC^[CT]
HapII 14 CCGG C^CGG
Hin1I 370 GGCGCC G[GA]^CG[CT]C
HpaII 14 CCGG C^CGG
KasI 370 GGCGCC G^GCGCC
Ksp632I 296 CTCTTC CTCTTC(1/4)
MluNI 341 TGGCCA TGG^CCA
MscI 341 TGGCCA TGG^CCA
MslI 168 CACCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 14 CCGG C^CGG
MwoI 97 GCCGTAGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 13 GCCGGC GCC^GGC
NarI 370 GGCGCC GG^CGCC
NgoMIV 13 GCCGGC G^CCGGC
NlaIII 81 CATG CATG^
PflFI 379 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 121 CACGTG CAC^GTG
PmlI 121 CACGTG CAC^GTG
SfaNI 27 GATGC GCATC(5/9)
SfoI 370 GGCGCC GGC^GCC
SmlI 58 CTCAAG C^T[CT][GA]AG
TaqI 47 TCGA T^CGA
Tth111I 379 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 47 TCGA T^CGA
XcmI 113 CCAAGCCGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 42 GAATTT [GA]^AATT[CT]
395 GAATTT
AluI 53 AGCT AG^CT
338 AGCT
ApoI 42 GAATTT [GA]^AATT[CT]
395 GAATTT
AviII 29 TGCGCA TGC^GCA
176 TGCGCA
BanI 16 GGCACC G^G[CT][GA]CC
370 GGCGCC
BsaJI 170 CCACGG C^C[ACGT][ACGT]GG
227 CCGTGG
BseRI 217 GAGGAG GAGGAG(10/8)
220 GAGGAG
BssKI 20 CCTGG ^CC[ACGT]GG
135 CCAGG
BstNI 20 CCTGG CC^[AT]GG
135 CCAGG
BstYI 128 AGATCC [GA]^GATC[CT]
278 AGATCC
Cfr13I 313 GGCCC G^G[ACGT]CC
329 GGACC
DdeI 85 CTGAG C^T[ACGT]AG
185 CTGAG
DsaI 170 CCACGG C^C[GA][CT]GG
227 CCGTGG
EaeI 95 TGGCCG [CT]^GGCC[GA]
341 TGGCCA
EcoRII 20 CCTGG ^CC[AT]GG
135 CCAGG
FspI 29 TGCGCA TGC^GCA
176 TGCGCA
HgaI 236 GACGC GACGC(5/10)
109 GCGTC
MboII 24 GAAGA GAAGA(8/7)
297 TCTTC
MflI 128 AGATCC [GA]^GATC[CT]
278 AGATCC
MvaI 20 CCTGG CC^[AT]GG
135 CCAGG
Sau96I 313 GGCCC G^G[ACGT]CC
329 GGACC
ScrFI 20 CCTGG CC^[ACGT]GG
135 CCAGG
Tsp509I 43 AATT ^AATT
396 AATT
XhoII 128 AGATCC [GA]^GATC[CT]
278 AGATCC
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AlwI BbvI BsgI BsiYI
BslI BsrI Cac8I CfoI DpnII
Fnu4HI HaeIII HhaI HinP1I ItaI
MaeII MboI MnlI NdeII NlaIV
Sau3AI TseI TspRI