Restriction map for
pFN21AE6425
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NsiI PacI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI Van91I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 1231 CGCG CG^CG
AciI 393 CCGC CCGC(-3/-1)
AfaI 248 GTAC GT^AC
Alw44I 1150 GTGCAC G^TGCAC
AlwNI 681 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1150 GTGCAC G^TGCAC
AspHI 1150 GTGCAC G[AT]GC[AT]^C
AspI 456 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1067 CTCGAG C^[CT]CG[GA]G
BanII 712 GAGCCC G[GA]GC[CT]^C
BcgI 1255 CGACTGGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 553 CTAG C^TAG
BsaWI 905 TCCGGT [AT]^CCGG[AT]
BseRI 806 CTCCTC GAGGAG(10/8)
BsiHKAI 1150 GTGCAC G[AT]GC[AT]^C
BsmFI 138 GTCCC GGGAC(10/14)
BsoBI 1067 CTCGAG C^[CT]CG[GA]G
BspMI 912 ACCTGC ACCTGC(4/8)
BstBI 1190 TTCGAA TT^CGAA
BstEII 908 GGTCACC G^GT[ACGT]ACC
BstPI 908 GGTCACC G^GT[ACGT]ACC
BstUI 1231 CGCG CG^CG
BstXI 743 CCAAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EarI 998 GAAGAG CTCTTC(1/4)
EcoO65I 908 GGTCACC G^GT[ACGT]ACC
EcoT14I 734 CCAAGG C^C[AT][AT]GG
FauI 392 CCCGC CCCGC(4/6)
HaeIII 1042 GGCC GG^CC
HapII 906 CCGG C^CGG
HgaI 369 GCGTC GACGC(5/10)
HindIII 324 AAGCTT A^AGCTT
HinfI 621 GATTC G^A[ACGT]TC
HpaII 906 CCGG C^CGG
Ksp632I 998 GAAGAG CTCTTC(1/4)
MaeI 553 CTAG C^TAG
MspA1I 99 CAGCTG C[AC]G^C[GT]G
MspI 906 CCGG C^CGG
MvnI 1231 CGCG CG^CG
NruI 1230 TCGCGA TCG^CGA
NspI 723 GCATGC [GA]CATG^[CT]
NspV 1190 TTCGAA TT^CGAA
PaeR7I 1067 CTCGAG C^TCGAG
PflFI 456 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 99 CAGCTG CAG^CTG
RsaI 248 GTAC GT^AC
SfuI 1190 TTCGAA TT^CGAA
SmlI 1067 CTCGAG C^T[CT][GA]AG
SphI 723 GCATGC GCATG^C
SspI 1225 AATATT AAT^ATT
StyI 734 CCAAGG C^C[AT][AT]GG
SwaI 843 ATTTAAAT ATTT^AAAT
TfiI 621 GATTC G^A[AT]TC
Tth111I 456 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1067 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 929 GAATTT [GA]^AATT[CT]
1005 AAATTT
ApoI 929 GAATTT [GA]^AATT[CT]
1005 AAATTT
Asp700I 568 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1100 GAAAACCTTC
BbsI 371 GTCTTC GAAGAC(2/6)
1294 GTCTTC
BbvI 98 GCAGC GCAGC(8/12)
720 GCTGC
BpuAI 371 GTCTTC GAAGAC(2/6)
1294 GTCTTC
BsaJI 141 CCCTGG C^C[ACGT][ACGT]GG
734 CCAAGG
BsrDI 729 CATTGC GCAATG(2/0)
916 GCAATG
DraI 291 TTTAAA TTT^AAA
844 TTTAAA
DraII 34 GGGTCCT [GA]G^G[ACGT]CC[CT]
737 AGGACCC
EcoO109I 34 GGGTCCT [GA]G^G[ACGT]CC[CT]
737 AGGACCC
Fnu4HI 98 GCAGC GC^[ACGT]GC
720 GCTGC
ItaI 98 GCAGC GC^[ACGT]GC
720 GCTGC
MaeII 942 ACGT A^CGT
1154 ACGT
MslI 85 CATAATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1271 CATTTCAGTG
PpuMI 34 GGGTCCT [GA]G^G[AT]CC[CT]
737 AGGACCC
TseI 98 GCAGC G^C[AT]GC
720 GCTGC
XmnI 568 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1100 GAAAACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AluI AlwI AvaII BmyI BpmI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII FokI HphI
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TaqI Tru9I Tsp45I Tsp509I TspRI
TthHB8I XhoII