Restriction map for
pFN21AE3909
##################################
# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EarI EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MflI MluI MroI MseI
MslI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
Tru9I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 466 GTCTAC GT^[AC][GT]AC
AlwNI 309 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 148 CCTGAGG CC^T[ACGT]AGG
Aor51HI 59 AGCGCT AGC^GCT
ApaI 530 GGGCCC GGGCC^C
AspHI 235 GTGCTC G[AT]GC[AT]^C
AspI 190 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 590 GGTGCC G^G[CT][GA]CC
BcnI 533 CCCGG CC^[GC]GG
BpmI 63 CTGGAG CTGGAG(16/14)
BsaI 655 GAGACC GGTCTC(1/5)
BseRI 201 CTCCTC GAGGAG(10/8)
BsgI 626 GTGCAG GTGCAG(16/14)
BsiHKAI 235 GTGCTC G[AT]GC[AT]^C
BsmAI 655 GAGAC GTCTC(1/5)
BsrI 114 CCAGT ACTGG(1/-1)
BstF5I 69 CATCC GGATG(2/0)
BstXI 554 CCATAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 148 CCTGAGG CC^T[ACGT]AGG
DpnII 816 GATC ^GATC
DraII 529 AGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 676 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 59 AGCGCT AGC^GCT
Eco81I 148 CCTGAGG CC^T[ACGT]AGG
EcoO109I 529 AGGGCCC [GA]G^G[ACGT]CC[CT]
FauI 726 GCGGG CCCGC(4/6)
FokI 69 CATCC GGATG(9/13)
HapII 534 CCGG C^CGG
HgaI 451 GACGC GACGC(5/10)
HpaII 534 CCGG C^CGG
HphI 17 TCACC GGTGA(8/7)
MaeII 680 ACGT A^CGT
MboI 816 GATC ^GATC
MspI 534 CCGG C^CGG
NciI 533 CCCGG CC^[GC]GG
NdeI 338 CATATG CA^TATG
NdeII 816 GATC ^GATC
PflFI 190 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 73 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 662 CTGCAG CTGCA^G
Sau3AI 816 GATC ^GATC
SfcI 662 CTGCAG C^T[GA][CT]AG
SmlI 853 CTCAAG C^T[CT][GA]AG
TfiI 515 GAATC G^A[AT]TC
Tsp509I 558 AATT ^AATT
Tth111I 190 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 73 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 117 GTAC GT^AC
883 GTAC
BalI 272 TGGCCA TGG^CCA
821 TGGCCA
BanII 106 GAGCCC G[GA]GC[CT]^C
530 GGGCCC
BsaJI 27 CCAGGG C^C[ACGT][ACGT]GG
787 CCCAGG
BsiYI 73 CCAGCTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
720 CCTTCGGCGGG
BslI 73 CCAGCTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
720 CCTTCGGCGGG
BsmFI 449 GGGAC GGGAC(10/14)
728 GGGAC
BspMI 286 GCAGGT ACCTGC(4/8)
441 GCAGGT
EaeI 272 TGGCCA [CT]^GGCC[GA]
821 TGGCCA
HaeII 59 AGCGCT [GA]GCGC^[CT]
800 GGCGCT
MaeIII 16 GTCAC ^GT[ACGT]AC
578 GTGAC
MboII 331 GAAGA GAAGA(8/7)
741 GAAGA
MluNI 272 TGGCCA TGG^CCA
821 TGGCCA
MscI 272 TGGCCA TGG^CCA
821 TGGCCA
MspA1I 74 CAGCTG C[AC]G^C[GT]G
665 CAGCTG
PvuII 74 CAGCTG CAG^CTG
665 CAGCTG
RsaI 117 GTAC GT^AC
883 GTAC
TaqI 494 TCGA T^CGA
653 TCGA
Tsp45I 16 GTCAC ^GT[GC]AC
578 GTGAC
TthHB8I 494 TCGA T^CGA
653 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI AvaII BbvI BcgI
BmyI Bsp1286I BssKI BstNI Cac8I
CfoI Cfr13I DdeI EcoRII Fnu4HI
HaeIII HhaI HinP1I HinfI ItaI
MnlI MvaI MwoI NlaIII NlaIV
NspI PleI Sau96I ScrFI SfaNI
SphI TseI TspRI