Restriction map for
pFN21AE4525
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# NO CUT ENZYMES ( 100 enzymes) #
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AatII AccI AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I AspI AviII AvrII
BbrPI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BsaI BsiWI
BsmBI BsmI Bsp1407I BspDI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI Eco47III EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KspI MaeI MfeI
MluI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StuI SwaI TfiI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 1507 GGTACC G^GTACC
AccII 1165 CGCG CG^CG
AccIII 1007 TCCGGA T^CCGGA
AflIII 1358 ACGTGT A^C[GA][CT]GT
AocI 1209 CCTCAGG CC^T[ACGT]AGG
ApaI 234 GGGCCC GGGCC^C
Asp718I 1507 GGTACC G^GTACC
AspHI 226 GAGCTC G[AT]GC[AT]^C
BamHI 1481 GGATCC G^GATCC
BbsI 1494 GAAGAC GAAGAC(2/6)
BcgI 1607 GCATCCACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 31 AGATCT A^GATCT
BpuAI 1494 GAAGAC GAAGAC(2/6)
BsaAI 778 TACGTG [CT]AC^GT[GA]
BsaBI 399 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1007 TCCGGA T^CCGGA
BsgI 928 GTGCAG GTGCAG(16/14)
BsiEI 245 CGGCCG CG[GA][CT]^CG
BsiHKAI 226 GAGCTC G[AT]GC[AT]^C
BspEI 1007 TCCGGA T^CCGGA
BspHI 1082 TCATGA T^CATGA
BsrBI 864 GAGCGG CCGCTC(-3/-3)
BsrI 1101 CCAGT ACTGG(1/-1)
BssSI 1456 CACGAG CACGAG(-5/-1)
BstUI 1165 CGCG CG^CG
Bsu36I 1209 CCTCAGG CC^T[ACGT]AGG
DraIII 1532 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1159 GACACCCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1658 CCATGG C^C[GA][CT]GG
EagI 245 CGGCCG C^GGCCG
EarI 898 CTCTTC CTCTTC(1/4)
EclXI 245 CGGCCG C^GGCCG
Eco52I 245 CGGCCG C^GGCCG
Eco81I 1209 CCTCAGG CC^T[ACGT]AGG
EcoRI 573 GAATTC G^AATTC
KpnI 1507 GGTACC GGTAC^C
Ksp632I 898 CTCTTC CTCTTC(1/4)
MaeIII 1451 GTGAC ^GT[ACGT]AC
MamI 399 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1007 TCCGGA T^CCGGA
MseI 886 TTAA T^TAA
MvnI 1165 CGCG CG^CG
NcoI 1658 CCATGG C^CATGG
NspI 1577 ACATGC [GA]CATG^[CT]
RcaI 1082 TCATGA T^CATGA
SacI 226 GAGCTC GAGCT^C
SbfI 1116 CCTGCAGG CCTGCA^GG
SexAI 1463 ACCAGGT A^CC[AT]GGT
Sse8387I 1116 CCTGCAGG CCTGCA^GG
Tru9I 886 TTAA T^TAA
Tsp45I 1451 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AhdI 283 GACATCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1336 GACCTGCTGTC
AlwNI 136 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
578 CAGAGCCTG
AspEI 283 GACATCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1336 GACCTGCTGTC
AvaI 231 CTCGGG C^[CT]CG[GA]G
463 CCCGAG
BalI 113 TGGCCA TGG^CCA
1661 TGGCCA
BbeI 537 GGCGCC GGCGC^C
735 GGCGCC
BbvCI 532 GCTGAGG CCTCAGC(-5/-2)
640 GCTGAGG
BglI 106 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
667 GCCACTGAGGC
BsmAI 55 GAGAC GTCTC(1/5)
594 GTCTC
BsoBI 231 CTCGGG C^[CT]CG[GA]G
463 CCCGAG
Eam1105I 283 GACATCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1336 GACCTGCTGTC
KasI 537 GGCGCC G^GCGCC
735 GGCGCC
MaeII 779 ACGT A^CGT
1358 ACGT
MluNI 113 TGGCCA TGG^CCA
1661 TGGCCA
MscI 113 TGGCCA TGG^CCA
1661 TGGCCA
MslI 846 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1353 CATCGACGTG
NarI 537 GGCGCC GG^CGCC
735 GGCGCC
PflMI 135 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
561 CCAGACCTTGG
PstI 601 CTGCAG CTGCA^G
1117 CTGCAG
PvuII 598 CAGCTG CAG^CTG
684 CAGCTG
SfcI 601 CTGCAG C^T[GA][CT]AG
1117 CTGCAG
SfoI 537 GGCGCC GGC^GCC
735 GGCGCC
SmlI 340 CTCAAG C^T[CT][GA]AG
727 CTCAAG
Van91I 135 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
561 CCAGACCTTGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaHI
BsaJI BsaWI BseRI BsiYI BslI
BsmFI Bsp1286I BspMI BsrFI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TseI Tsp509I
TspRI TthHB8I XhoII