Restriction map for
pFN21AE1510
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BalI BanII BbeI BbvCI BcgI
BciVI BclI BglI BglII BlnI
BlpI Bpu1102I BsaHI BsaI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspHI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI KspI MluI MluNI
MscI NaeI NarI NcoI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I XbaI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 5 CGCG CG^CG
AccIII 1544 TCCGGA T^CCGGA
AflII 1731 CTTAAG C^TTAAG
AlwNI 227 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 103 AGCGCT AGC^GCT
AvaI 32 CCCGGG C^[CT]CG[GA]G
BbrPI 1314 CACGTG CAC^GTG
BfrI 1731 CTTAAG C^TTAAG
BsaAI 1314 CACGTG [CT]AC^GT[GA]
BsaWI 1544 TCCGGA [AT]^CCGG[AT]
BseAI 1544 TCCGGA T^CCGGA
BsoBI 32 CCCGGG C^[CT]CG[GA]G
BspEI 1544 TCCGGA T^CCGGA
BspLU11I 420 ACATGT A^CATGT
BsrBI 1468 CCGCTC CCGCTC(-3/-3)
BstUI 5 CGCG CG^CG
BstXI 1568 CCAGAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 115 GACTTCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 858 CCACGG C^C[GA][CT]GG
EaeI 152 CGGCCA [CT]^GGCC[GA]
Eco47III 103 AGCGCT AGC^GCT
HaeII 103 AGCGCT [GA]GCGC^[CT]
HincII 1231 GTTGAC GT[CT]^[GA]AC
HindII 1231 GTTGAC GT[CT]^[GA]AC
MfeI 2069 CAATTG C^AATTG
MroI 1544 TCCGGA T^CCGGA
MslI 780 CATGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2069 CAATTG C^AATTG
MvnI 5 CGCG CG^CG
NdeI 1244 CATATG CA^TATG
NheI 1672 GCTAGC G^CTAGC
NspI 420 ACATGT [GA]CATG^[CT]
PflMI 2135 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1314 CACGTG CAC^GTG
PmlI 1314 CACGTG CAC^GTG
PstI 55 CTGCAG CTGCA^G
SmaI 32 CCCGGG CCC^GGG
Van91I 2135 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 32 CCCGGG C^CCGGG
XmaI 32 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AfaI 594 GTAC GT^AC
2028 GTAC
AflIII 420 ACATGT A^C[GA][CT]GT
1313 ACACGT
Asp700I 940 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1885 GAAGAGATTC
AvaII 855 GGACC G^G[AT]CC
1385 GGTCC
BamHI 1223 GGATCC G^GATCC
1736 GGATCC
BbsI 930 GAAGAC GAAGAC(2/6)
1828 GAAGAC
BcnI 32 CCCGG CC^[GC]GG
33 CCGGG
BfaI 1673 CTAG C^TAG
1942 CTAG
BmyI 88 GTGCCC G[AGT]GC[ACT]^C
94 GTGCCC
BpuAI 930 GAAGAC GAAGAC(2/6)
1828 GAAGAC
BsaBI 898 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1045 GATAAAGATC
BsmFI 470 GGGAC GGGAC(10/14)
1386 GTCCC
BsmI 841 GAATGC GAATGC(1/-1)
587 GCATTC
Bsp1286I 88 GTGCCC G[AGT]GC[ACT]^C
94 GTGCCC
BspMI 1515 ACCTGC ACCTGC(4/8)
57 GCAGGT
BsrDI 823 GCAATG GCAATG(2/0)
925 GCAATG
BssSI 1337 CACGAG CACGAG(-5/-1)
540 CTCGTG
CfoI 4 GCGC GCG^C
104 GCGC
HaeIII 153 GGCC GG^CC
2089 GGCC
HapII 33 CCGG C^CGG
1545 CCGG
HhaI 4 GCGC GCG^C
104 GCGC
HinP1I 4 GCGC G^CGC
104 GCGC
HpaII 33 CCGG C^CGG
1545 CCGG
MaeI 1673 CTAG C^TAG
1942 CTAG
MamI 898 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1045 GATAAAGATC
MspA1I 227 CAGCTG C[AC]G^C[GT]G
935 CAGCTG
MspI 33 CCGG C^CGG
1545 CCGG
NciI 32 CCCGG CC^[GC]GG
33 CCGGG
PleI 307 GAGTC GAGTC(4/5)
1415 GAGTC
PvuII 227 CAGCTG CAG^CTG
935 CAGCTG
RsaI 594 GTAC GT^AC
2028 GTAC
SexAI 137 ACCTGGT A^CC[AT]GGT
572 ACCAGGT
SfcI 55 CTGCAG C^T[GA][CT]AG
949 CTACAG
SmlI 1731 CTTAAG C^T[CT][GA]AG
2032 CTTGAG
XmnI 940 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1885 GAAGAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BanI BbvI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EarI EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HinfI HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII