Restriction map for
pFN21AE1314
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AvaI AvaII AviII AvrII
BalI BanI BbeI BbrPI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 931 CGCG CG^CG
AspHI 940 GTGCTC G[AT]GC[AT]^C
AspI 1360 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 495 GGATCC G^GATCC
BbvCI 289 GCTGAGG CCTCAGC(-5/-2)
BbvI 709 GCAGC GCAGC(8/12)
BcnI 546 CCGGG CC^[GC]GG
BpmI 141 CTGGAG CTGGAG(16/14)
BsaI 1049 GGTCTC GGTCTC(1/5)
BseRI 777 CTCCTC GAGGAG(10/8)
BsiHKAI 940 GTGCTC G[AT]GC[AT]^C
BsmFI 416 GTCCC GGGAC(10/14)
BstNI 140 CCTGG CC^[AT]GG
BstUI 931 CGCG CG^CG
BstYI 495 GGATCC [GA]^GATC[CT]
Cfr13I 1373 GGGCC G^G[ACGT]CC
DraI 670 TTTAAA TTT^AAA
EcoRII 140 CCTGG ^CC[AT]GG
Fnu4HI 709 GCAGC GC^[ACGT]GC
HaeIII 1374 GGCC GG^CC
HgaI 238 GACGC GACGC(5/10)
HincII 1366 GTCAAC GT[CT]^[GA]AC
HindII 1366 GTCAAC GT[CT]^[GA]AC
ItaI 709 GCAGC GC^[ACGT]GC
MaeII 593 ACGT A^CGT
MflI 495 GGATCC [GA]^GATC[CT]
MvaI 140 CCTGG CC^[AT]GG
MvnI 931 CGCG CG^CG
NciI 546 CCGGG CC^[GC]GG
NruI 930 TCGCGA TCG^CGA
PflFI 1360 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Sau96I 1373 GGGCC G^G[ACGT]CC
SspI 1001 AATATT AAT^ATT
TfiI 1557 GATTC G^A[AT]TC
TseI 709 GCAGC G^C[AT]GC
Tth111I 1360 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 495 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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Asp700I 627 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1324 GAAAAGCTTC
BfaI 1054 CTAG C^TAG
1657 CTAG
BsiYI 546 CCGGGATCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1097 CCATGAAGCGG
BslI 546 CCGGGATCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1097 CCATGAAGCGG
BssKI 140 CCTGG ^CC[ACGT]GG
546 CCGGG
EarI 1377 CTCTTC CTCTTC(1/4)
442 GAAGAG
EcoT14I 586 CCTTGG C^C[AT][AT]GG
1140 CCTTGG
EcoT22I 333 ATGCAT ATGCA^T
1307 ATGCAT
HapII 12 CCGG C^CGG
546 CCGG
HpaII 12 CCGG C^CGG
546 CCGG
HphI 361 GGTGA GGTGA(8/7)
1247 TCACC
Ksp632I 1377 CTCTTC CTCTTC(1/4)
442 GAAGAG
MaeI 1054 CTAG C^TAG
1657 CTAG
MslI 336 CATAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1150 CATCTGGATG
MspI 12 CCGG C^CGG
546 CCGG
NsiI 333 ATGCAT ATGCA^T
1307 ATGCAT
NspI 885 GCATGT [GA]CATG^[CT]
1534 ACATGC
PstI 126 CTGCAG CTGCA^G
423 CTGCAG
ScrFI 140 CCTGG CC^[ACGT]GG
546 CCGGG
StyI 586 CCTTGG C^C[AT][AT]GG
1140 CCTTGG
XmnI 627 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1324 GAAAAGCTTC
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanII BbsI BmyI BpuAI
BsaJI BsmAI Bsp1286I BsrI BstF5I
Cac8I DdeI DpnII FokI HindIII
HinfI MaeIII MboI MboII MnlI
MseI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI SfaNI SfcI
Tru9I Tsp45I Tsp509I TspRI