Restriction map for
pFN21AE1448
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DraI DraII
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I FbaI FseI
FspI HindIII HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tth111I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 729 CGCG CG^CG
AcsI 184 GAATTC [GA]^AATT[CT]
AcyI 14 GACGCC G[GA]^CG[CT]C
AfaI 855 GTAC GT^AC
AlwNI 712 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 631 GGGCCC GGGCC^C
ApoI 184 GAATTC [GA]^AATT[CT]
AvaI 18 CCCGGG C^[CT]CG[GA]G
BanI 3 GGCACC G^G[CT][GA]CC
BbsI 648 GAAGAC GAAGAC(2/6)
BglI 826 GCCAGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 648 GAAGAC GAAGAC(2/6)
BsaHI 14 GACGCC G[GA]^CG[CT]C
BseRI 907 GAGGAG GAGGAG(10/8)
BsgI 709 GTGCAG GTGCAG(16/14)
BsmAI 545 GAGAC GTCTC(1/5)
BsoBI 18 CCCGGG C^[CT]CG[GA]G
BspHI 662 TCATGA T^CATGA
BspMI 6 ACCTGC ACCTGC(4/8)
BstF5I 369 GGATG GGATG(2/0)
BstUI 729 CGCG CG^CG
BstXI 293 CCAGGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1055 GACCTTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 512 CCGTGG C^C[GA][CT]GG
EarI 102 GAAGAG CTCTTC(1/4)
EcoRI 184 GAATTC G^AATTC
EcoT22I 550 ATGCAT ATGCA^T
FauI 863 GCGGG CCCGC(4/6)
FokI 369 GGATG GGATG(9/13)
HaeII 44 AGCGCC [GA]GCGC^[CT]
Hin1I 14 GACGCC G[GA]^CG[CT]C
HincII 170 GTCAAC GT[CT]^[GA]AC
HindII 170 GTCAAC GT[CT]^[GA]AC
Ksp632I 102 GAAGAG CTCTTC(1/4)
MaeII 461 ACGT A^CGT
MfeI 899 CAATTG C^AATTG
MspA1I 960 CAGCTG C[AC]G^C[GT]G
MunI 899 CAATTG C^AATTG
MvnI 729 CGCG CG^CG
NsiI 550 ATGCAT ATGCA^T
PshAI 782 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 960 CAGCTG CAG^CTG
RcaI 662 TCATGA T^CATGA
RsaI 855 GTAC GT^AC
SacI 658 GAGCTC GAGCT^C
SfaNI 316 GATGC GCATC(5/9)
SmaI 18 CCCGGG CCC^GGG
StuI 833 AGGCCT AGG^CCT
Tsp45I 284 GTGAC ^GT[GC]AC
XmaCI 18 CCCGGG C^CCGGG
XmaI 18 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 624 CCGC CCGC(-3/-1)
863 GCGG
AspHI 457 GAGCAC G[AT]GC[AT]^C
658 GAGCTC
BfaI 377 CTAG C^TAG
406 CTAG
BsiHKAI 457 GAGCAC G[AT]GC[AT]^C
658 GAGCTC
BsrI 783 ACTGG ACTGG(1/-1)
233 CCAGT
BstYI 335 GGATCT [GA]^GATC[CT]
759 GGATCT
HapII 19 CCGG C^CGG
566 CCGG
HgaI 14 GACGC GACGC(5/10)
160 GACGC
HpaII 19 CCGG C^CGG
566 CCGG
HphI 313 GGTGA GGTGA(8/7)
588 GGTGA
MaeI 377 CTAG C^TAG
406 CTAG
MaeIII 284 GTGAC ^GT[ACGT]AC
520 GTTAC
MflI 335 GGATCT [GA]^GATC[CT]
759 GGATCT
MseI 383 TTAA T^TAA
493 TTAA
MspI 19 CCGG C^CGG
566 CCGG
NspI 548 ACATGC [GA]CATG^[CT]
980 ACATGC
PflMI 800 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
989 CCAAACCCTGG
PleI 132 GACTC GAGTC(4/5)
787 GAGTC
SmlI 189 CTTGAG C^T[CT][GA]AG
676 CTTGAG
TaqI 396 TCGA T^CGA
1014 TCGA
TfiI 499 GAATC G^A[AT]TC
1032 GAATC
Tru9I 383 TTAA T^TAA
493 TTAA
TspRI 143 CAGTG CA[GC]TG[ACGT][ACGT]^
234 CAGTG
TthHB8I 396 TCGA T^CGA
1014 TCGA
Van91I 800 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
989 CCAAACCCTGG
XhoII 335 GGATCT [GA]^GATC[CT]
759 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AluI AlwI BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
Bsp1286I BssKI BstNI Cac8I CfoI
Cfr13I DpnII EcoRII Fnu4HI HaeIII
HhaI HinP1I HinfI ItaI MboI
MboII MnlI MvaI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI TseI Tsp509I