Restriction map for
pFN21AE6315
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Alw44I AlwNI
AocI ApaLI ApoI AscI AseI
AsnI Asp718I AspI AviII BalI
BamHI BbrPI BclI BfrI BglII
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspMI BsrDI BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HindIII HpaI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI TfiI Tth111I
TthHB8I Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AhdI 990 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 426 AGCGCT AGC^GCT
ApaI 84 GGGCCC GGGCC^C
AspEI 990 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 511 GTGCTC G[AT]GC[AT]^C
AvaI 148 CTCGGG C^[CT]CG[GA]G
AvrII 699 CCTAGG C^CTAGG
BbsI 754 GTCTTC GAAGAC(2/6)
BbvCI 585 CCTCAGC CCTCAGC(-5/-2)
BciVI 884 GTATCC GTATCC(6/5)
BlnI 699 CCTAGG C^CTAGG
BlpI 141 GCTCAGC GC^T[ACGT]AGC
BpmI 117 CTCCAG CTGGAG(16/14)
Bpu1102I 141 GCTCAGC GC^T[ACGT]AGC
BpuAI 754 GTCTTC GAAGAC(2/6)
BsaI 812 GGTCTC GGTCTC(1/5)
BseRI 606 CTCCTC GAGGAG(10/8)
BsiHKAI 511 GTGCTC G[AT]GC[AT]^C
BsmAI 813 GTCTC GTCTC(1/5)
BsoBI 148 CTCGGG C^[CT]CG[GA]G
BspHI 596 TCATGA T^CATGA
BspLU11I 1082 ACATGT A^CATGT
BsrBI 1295 CCGCTC CCGCTC(-3/-3)
BssHII 413 GCGCGC G^CGCGC
CelII 141 GCTCAGC GC^T[ACGT]AGC
EaeI 497 TGGCCG [CT]^GGCC[GA]
Eam1105I 990 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 426 AGCGCT AGC^GCT
EcoNI 922 CCTTTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 699 CCTAGG C^C[AT][AT]GG
HgaI 752 GCGTC GACGC(5/10)
HincII 568 GTTGAC GT[CT]^[GA]AC
HindII 568 GTTGAC GT[CT]^[GA]AC
HphI 344 TCACC GGTGA(8/7)
MseI 1138 TTAA T^TAA
NaeI 124 GCCGGC GCC^GGC
NgoMIV 124 GCCGGC G^CCGGC
PpuMI 864 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 596 TCATGA T^CATGA
SapI 27 GCTCTTC GCTCTTC(1/4)
StyI 699 CCTAGG C^C[AT][AT]GG
Tru9I 1138 TTAA T^TAA
TspRI 1262 CAGTG CA[GC]TG[ACGT][ACGT]^
XbaI 1185 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 1031 GTAC GT^AC
1080 GTAC
AflIII 317 ACGTGT A^C[GA][CT]GT
1082 ACATGT
Asp700I 315 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
378 GAACCACTTC
BcgI 92 GCACGGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
393 GCACGTCCGTCG
Bsp1407I 1030 TGTACA T^GTACA
1079 TGTACA
BsrFI 124 GCCGGC [GA]^CCGG[CT]
850 ACCGGC
BsrGI 1030 TGTACA T^GTACA
1079 TGTACA
BstXI 74 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
611 CCACGCTTCTGG
BstYI 650 GGATCT [GA]^GATC[CT]
1179 AGATCC
Cfr10I 124 GCCGGC [GA]^CCGG[CT]
850 ACCGGC
DraII 84 GGGCCCC [GA]G^G[ACGT]CC[CT]
864 AGGACCT
EcoO109I 84 GGGCCCC [GA]G^G[ACGT]CC[CT]
864 AGGACCT
HinfI 717 GACTC G^A[ACGT]TC
825 GACTC
MflI 650 GGATCT [GA]^GATC[CT]
1179 AGATCC
NspI 1076 GCATGT [GA]CATG^[CT]
1082 ACATGT
PleI 717 GACTC GAGTC(4/5)
825 GACTC
PvuII 657 CAGCTG CAG^CTG
893 CAGCTG
RsaI 1031 GTAC GT^AC
1080 GTAC
SspBI 1030 TGTACA T^GTACA
1079 TGTACA
Tsp509I 912 AATT ^AATT
1064 AATT
XcmI 347 CCATGCTGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1037 CCATTCTAAAACTGG
XhoII 650 GGATCT [GA]^GATC[CT]
1179 AGATCC
XmnI 315 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
378 GAACCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AvaII BanI BanII BbeI BbvI
BcnI BfaI BglI BmyI BsaHI
BsaJI BsgI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EarI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PstI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI TseI
Tsp45I