Restriction map for
pFN21AE3625
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsiEI BsiWI BsmI BsoBI BspDI
BspHI BspLU11I BspMI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoT22I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MaeII MamI MflI
MluI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StuI
SwaI Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 643 TCCGGA T^CCGGA
AcsI 309 AAATTT [GA]^AATT[CT]
AflII 879 CTTAAG C^TTAAG
ApoI 309 AAATTT [GA]^AATT[CT]
AseI 429 ATTAAT AT^TAAT
AsnI 429 ATTAAT AT^TAAT
AspI 948 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 910 TGGCCA TGG^CCA
BanII 187 GAGCTC G[GA]GC[CT]^C
BcnI 794 CCGGG CC^[GC]GG
BfaI 142 CTAG C^TAG
BfrI 879 CTTAAG C^TTAAG
BsaI 1036 GGTCTC GGTCTC(1/5)
BsaWI 643 TCCGGA [AT]^CCGG[AT]
BseAI 643 TCCGGA T^CCGGA
BseRI 151 CTCCTC GAGGAG(10/8)
BsmBI 246 CGTCTC CGTCTC(1/5)
BspEI 643 TCCGGA T^CCGGA
BsrBI 726 GAGCGG CCGCTC(-3/-3)
BstEII 810 GGTCACC G^GT[ACGT]ACC
BstPI 810 GGTCACC G^GT[ACGT]ACC
DraII 652 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1336 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 910 TGGCCA [CT]^GGCC[GA]
EcoO109I 652 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 810 GGTCACC G^GT[ACGT]ACC
EcoT14I 815 CCAAGG C^C[AT][AT]GG
FauI 159 GCGGG CCCGC(4/6)
HgaI 343 GACGC GACGC(5/10)
MaeI 142 CTAG C^TAG
MboII 357 GAAGA GAAGA(8/7)
MfeI 914 CAATTG C^AATTG
MluNI 910 TGGCCA TGG^CCA
MroI 643 TCCGGA T^CCGGA
MscI 910 TGGCCA TGG^CCA
MspA1I 569 CAGCGG C[AC]G^C[GT]G
MunI 914 CAATTG C^AATTG
NciI 794 CCGGG CC^[GC]GG
PflFI 948 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 652 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 429 ATTAAT AT^TAAT
SacI 187 GAGCTC GAGCT^C
SexAI 1337 ACCTGGT A^CC[AT]GGT
SphI 1316 GCATGC GCATG^C
StyI 815 CCAAGG C^C[AT][AT]GG
TaqI 215 TCGA T^CGA
TfiI 331 GATTC G^A[AT]TC
Tth111I 948 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 215 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 217 GATCC GGATC(4/5)
87 GGATC
AvaII 402 GGACC G^G[AT]CC
653 GGACC
BsaJI 182 CCCTGG C^C[ACGT][ACGT]GG
815 CCAAGG
BsgI 566 GTGCAG GTGCAG(16/14)
884 GTGCAG
Cfr13I 402 GGACC G^G[ACGT]CC
653 GGACC
DpnII 88 GATC ^GATC
217 GATC
EcoRV 715 GATATC GAT^ATC
1126 GATATC
HapII 644 CCGG C^CGG
794 CCGG
HindIII 592 AAGCTT A^AGCTT
1239 AAGCTT
HpaII 644 CCGG C^CGG
794 CCGG
MboI 88 GATC ^GATC
217 GATC
MspI 644 CCGG C^CGG
794 CCGG
NdeII 88 GATC ^GATC
217 GATC
PleI 147 GACTC GAGTC(4/5)
1086 GACTC
PstI 734 CTGCAG CTGCA^G
982 CTGCAG
Sau3AI 88 GATC ^GATC
217 GATC
Sau96I 402 GGACC G^G[ACGT]CC
653 GGACC
Tsp45I 598 GTGAC ^GT[GC]AC
811 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI AspHI BbvI
BmyI BpmI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI Cac8I
DdeI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeIII MnlI
MseI MslI MvaI MwoI NlaIII
NlaIV NspI RsaI ScrFI SfaNI
SfcI SmlI SspBI Tru9I TseI
Tsp509I TspRI