Restriction map for
pFN21AE1505
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvrII BalI
BamHI BanII BbrPI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BsaAI BsaBI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstXI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HinfI KpnI
MaeI MamI MfeI MflI MluI
MluNI MscI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
PacI PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI SfiI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tsp509I Tth111I
XbaI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 1397 TCCGGA T^CCGGA
AcyI 1228 GGCGCC G[GA]^CG[CT]C
AflIII 833 ACGTGT A^C[GA][CT]GT
Alw44I 668 GTGCAC G^TGCAC
ApaLI 668 GTGCAC G^TGCAC
Asp700I 171 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1116 TGCGCA TGC^GCA
BbeI 1228 GGCGCC GGCGC^C
BcgI 627 CGAGGAGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 580 GCCACGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1449 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1449 GCTGAGC GC^T[ACGT]AGC
BsaHI 1228 GGCGCC G[GA]^CG[CT]C
BsaI 1336 GAGACC GGTCTC(1/5)
BseAI 1397 TCCGGA T^CCGGA
BsmAI 1336 GAGAC GTCTC(1/5)
BsmFI 206 GGGAC GGGAC(10/14)
BspEI 1397 TCCGGA T^CCGGA
BssHII 1278 GCGCGC G^CGCGC
BssSI 701 CTCGTG CACGAG(-5/-1)
BstBI 787 TTCGAA TT^CGAA
BstEII 16 GGTGACC G^GT[ACGT]ACC
BstPI 16 GGTGACC G^GT[ACGT]ACC
CelII 1449 GCTGAGC GC^T[ACGT]AGC
DrdI 1075 GACCTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 790 GAAGAG CTCTTC(1/4)
EcoO65I 16 GGTGACC G^GT[ACGT]ACC
FspI 1116 TGCGCA TGC^GCA
Hin1I 1228 GGCGCC G[GA]^CG[CT]C
HincII 268 GTTAAC GT[CT]^[GA]AC
HindII 268 GTTAAC GT[CT]^[GA]AC
HpaI 268 GTTAAC GTT^AAC
KasI 1228 GGCGCC G^GCGCC
Ksp632I 790 GAAGAG CTCTTC(1/4)
MroI 1397 TCCGGA T^CCGGA
MseI 269 TTAA T^TAA
NaeI 517 GCCGGC GCC^GGC
NarI 1228 GGCGCC GG^CGCC
NgoMIV 517 GCCGGC G^CCGGC
NspV 787 TTCGAA TT^CGAA
PstI 1001 CTGCAG CTGCA^G
SapI 790 GAAGAGC GCTCTTC(1/4)
ScaI 1481 AGTACT AGT^ACT
SexAI 1076 ACCTGGT A^CC[AT]GGT
SfoI 1228 GGCGCC GGC^GCC
SfuI 787 TTCGAA TT^CGAA
SgrAI 1049 CGCCGGTG C[GA]^CCGG[CT]G
Tru9I 269 TTAA T^TAA
XcmI 938 CCAAGGGCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 171 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwI 393 GATCC GGATC(4/5)
774 GATCC
AvaI 6 CTCGAG C^[CT]CG[GA]G
1477 CTCGAG
AvaII 447 GGACC G^G[AT]CC
1074 GGACC
BbsI 460 GTCTTC GAAGAC(2/6)
1186 GTCTTC
BpuAI 460 GTCTTC GAAGAC(2/6)
1186 GTCTTC
BsaWI 20 ACCGGA [AT]^CCGG[AT]
1397 TCCGGA
BseRI 628 GAGGAG GAGGAG(10/8)
1333 GAGGAG
BsoBI 6 CTCGAG C^[CT]CG[GA]G
1477 CTCGAG
BsrBI 793 GAGCGG CCGCTC(-3/-3)
1362 GAGCGG
DraII 361 GGGGCCT [GA]G^G[ACGT]CC[CT]
1064 AGGCCCT
EcoO109I 361 GGGGCCT [GA]G^G[ACGT]CC[CT]
1064 AGGCCCT
HaeII 642 GGCGCT [GA]GCGC^[CT]
1228 GGCGCC
PaeR7I 6 CTCGAG C^TCGAG
1477 CTCGAG
PflMI 249 CCAGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
351 CCACAGTCTGG
SfcI 366 CTACAG C^T[GA][CT]AG
1001 CTGCAG
SmlI 6 CTCGAG C^T[CT][GA]AG
1477 CTCGAG
Van91I 249 CCAGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
351 CCACAGTCTGG
XhoI 6 CTCGAG C^TCGAG
1477 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI BanI BbvI BcnI BmyI
BpmI BsaJI BsgI BsiHKAI BsiYI
BslI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EcoNI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI KspI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI Tsp45I TspRI
TthHB8I