Restriction map for
pFN21AE6311
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BalI BbrPI
BcgI BciVI BfaI BfrI BlnI
BsaAI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I ClaI DraI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
HincII HindII HindIII HpaI KpnI
KspI MaeI MaeII MfeI MluI
MluNI MroI MscI MseI MunI
MvnI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tsp45I Tsp509I Tth111I XbaI
XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AviII 710 TGCGCA TGC^GCA
BbeI 259 GGCGCC GGCGC^C
BbsI 204 GAAGAC GAAGAC(2/6)
BbvCI 62 CCTCAGC CCTCAGC(-5/-2)
BclI 401 TGATCA T^GATCA
BglII 683 AGATCT A^GATCT
BlpI 582 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 582 GCTGAGC GC^T[ACGT]AGC
BpuAI 204 GAAGAC GAAGAC(2/6)
BstAPI 702 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1210 CCACCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 582 GCTGAGC GC^T[ACGT]AGC
CpoI 98 CGGACCG CG^G[AT]CCG
DraIII 211 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 37 GACTGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 401 TGATCA T^GATCA
FspI 710 TGCGCA TGC^GCA
HaeII 259 GGCGCC [GA]GCGC^[CT]
HphI 789 GGTGA GGTGA(8/7)
KasI 259 GGCGCC G^GCGCC
MaeIII 411 GTTAC ^GT[ACGT]AC
MslI 183 CATTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 259 GGCGCC GG^CGCC
NspI 396 GCATGT [GA]CATG^[CT]
PaeR7I 1171 CTCGAG C^TCGAG
PflMI 641 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsrII 98 CGGACCG CG^G[AT]CCG
SfoI 259 GGCGCC GGC^GCC
SmaI 107 CCCGGG CCC^GGG
SmlI 1171 CTCGAG C^T[CT][GA]AG
Van91I 641 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1171 CTCGAG C^TCGAG
XmaCI 107 CCCGGG C^CCGGG
XmaI 107 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AcyI 3 GGCGTC G[GA]^CG[CT]C
259 GGCGCC
Alw44I 174 GTGCAC G^TGCAC
904 GTGCAC
ApaI 560 GGGCCC GGGCC^C
1205 GGGCCC
ApaLI 174 GTGCAC G^TGCAC
904 GTGCAC
BamHI 548 GGATCC G^GATCC
885 GGATCC
BanI 259 GGCGCC G^G[CT][GA]CC
664 GGTGCC
BglI 67 GCCATCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
382 GCCCAGGAGGC
BsaBI 795 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1114 GATCAGGATC
BsaHI 3 GGCGTC G[GA]^CG[CT]C
259 GGCGCC
BsgI 539 CTGCAC GTGCAG(16/14)
118 GTGCAG
BsrFI 164 GCCGGC [GA]^CCGG[CT]
1195 GCCGGC
Cfr10I 164 GCCGGC [GA]^CCGG[CT]
1195 GCCGGC
DsaI 854 CCACGG C^C[GA][CT]GG
1077 CCGTGG
EaeI 857 CGGCCA [CT]^GGCC[GA]
1193 TGGCCG
EarI 1234 CTCTTC CTCTTC(1/4)
1251 GAAGAG
EcoNI 876 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1233 CCTCTTCCAGG
EcoT14I 641 CCAAGG C^C[AT][AT]GG
725 CCAAGG
HgaI 207 GACGC GACGC(5/10)
4 GCGTC
Hin1I 3 GGCGTC G[GA]^CG[CT]C
259 GGCGCC
Ksp632I 1234 CTCTTC CTCTTC(1/4)
1251 GAAGAG
MamI 795 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1114 GATCAGGATC
MspA1I 521 CAGCTG C[AC]G^C[GT]G
580 CAGCTG
NaeI 164 GCCGGC GCC^GGC
1195 GCCGGC
NgoMIV 164 GCCGGC G^CCGGC
1195 GCCGGC
PvuII 521 CAGCTG CAG^CTG
580 CAGCTG
StyI 641 CCAAGG C^C[AT][AT]GG
725 CCAAGG
TfiI 219 GATTC G^A[AT]TC
1040 GATTC
TspRI 172 CAGTG CA[GC]TG[ACGT][ACGT]^
498 CACTG
XcmI 579 CCAGCTGAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
854 CCACGGCCAGCATGG
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MboI MboII
MflI MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI StuI
TaqI TseI TthHB8I XhoII