Restriction map for
pFN21AB7892
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI AscI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BciVI BfrI BlnI
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV FauI
FseI FspI HaeII HhaI Hin1I
HinP1I HindIII KasI KpnI KspI
MaeII MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NgoMIV NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tsp45I Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Alw44I 1670 GTGCAC G^TGCAC
ApaI 1378 GGGCCC GGGCC^C
ApaLI 1670 GTGCAC G^TGCAC
AseI 1164 ATTAAT AT^TAAT
AsnI 1164 ATTAAT AT^TAAT
AspHI 1670 GTGCAC G[AT]GC[AT]^C
BamHI 1587 GGATCC G^GATCC
BbsI 509 GAAGAC GAAGAC(2/6)
BglI 1416 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 38 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 38 GCTCAGC GC^T[ACGT]AGC
BpuAI 509 GAAGAC GAAGAC(2/6)
BsaBI 205 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 713 GAGACC GGTCTC(1/5)
BsiHKAI 1670 GTGCAC G[AT]GC[AT]^C
BsmAI 713 GAGAC GTCTC(1/5)
BspHI 566 TCATGA T^CATGA
BstBI 1025 TTCGAA TT^CGAA
CelII 38 GCTCAGC GC^T[ACGT]AGC
DraIII 1112 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1061 GACCTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 159 GAATTC G^AATTC
HgaI 85 GACGC GACGC(5/10)
MamI 205 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 1508 CATATG CA^TATG
NheI 577 GCTAGC G^CTAGC
NspI 996 ACATGC [GA]CATG^[CT]
NspV 1025 TTCGAA TT^CGAA
PleI 247 GACTC GAGTC(4/5)
PshAI 985 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1164 ATTAAT AT^TAAT
RcaI 566 TCATGA T^CATGA
SbfI 1663 CCTGCAGG CCTGCA^GG
ScaI 1760 AGTACT AGT^ACT
SfiI 1415 GGCCTCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1025 TTCGAA TT^CGAA
Sse8387I 1663 CCTGCAGG CCTGCA^GG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 1369 GTAC GT^AC
1761 GTAC
BclI 765 TGATCA T^GATCA
887 TGATCA
BfaI 578 CTAG C^TAG
1951 CTAG
BglII 289 AGATCT A^GATCT
362 AGATCT
BsmI 1895 GAATGC GAATGC(1/-1)
920 GCATTC
BspMI 1666 GCAGGT ACCTGC(4/8)
1925 GCAGGT
BstAPI 1657 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1783 GCAGTTCCTGC
BstXI 737 CCAAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1206 CCAGCAGTCTGG
EcoT22I 274 ATGCAT ATGCA^T
1532 ATGCAT
FbaI 765 TGATCA T^GATCA
887 TGATCA
HpaI 79 GTTAAC GTT^AAC
444 GTTAAC
MaeI 578 CTAG C^TAG
1951 CTAG
MaeIII 264 GTTAC ^GT[ACGT]AC
483 GTTAC
MslI 532 CATGGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1916 CACCTCAGTG
MspA1I 41 CAGCTG C[AC]G^C[GT]G
1724 CAGCTG
NsiI 274 ATGCAT ATGCA^T
1532 ATGCAT
PpuMI 433 AGGTCCT [GA]G^G[AT]CC[CT]
975 AGGACCT
PvuII 41 CAGCTG CAG^CTG
1724 CAGCTG
RsaI 1369 GTAC GT^AC
1761 GTAC
SexAI 1062 ACCTGGT A^CC[AT]GGT
1113 ACCTGGT
TaqI 1026 TCGA T^CGA
1071 TCGA
TthHB8I 1026 TCGA T^CGA
1071 TCGA
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp509I
TspRI Van91I XcmI XhoII