Restriction map for
pFN21AE2166
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbvCI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstZ17I CelII ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SalI SbfI SexAI SfcI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
Tsp509I Tth111I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AflIII 944 ACGTGT A^C[GA][CT]GT
AocI 841 CCTGAGG CC^T[ACGT]AGG
ApaI 747 GGGCCC GGGCC^C
AvaI 901 CCCGAG C^[CT]CG[GA]G
BbeI 40 GGCGCC GGCGC^C
BbsI 769 GTCTTC GAAGAC(2/6)
BcgI 968 GCACGCGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 769 GTCTTC GAAGAC(2/6)
BsaWI 257 ACCGGA [AT]^CCGG[AT]
BseRI 904 GAGGAG GAGGAG(10/8)
BsmBI 99 CGTCTC CGTCTC(1/5)
BsoBI 901 CCCGAG C^[CT]CG[GA]G
BspHI 107 TCATGA T^CATGA
BsrFI 674 GCCGGC [GA]^CCGG[CT]
BstEII 739 GGTCACC G^GT[ACGT]ACC
BstPI 739 GGTCACC G^GT[ACGT]ACC
BstXI 614 CCAACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 841 CCTGAGG CC^T[ACGT]AGG
Cfr10I 674 GCCGGC [GA]^CCGG[CT]
DraII 746 AGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 121 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 957 GAAGAG CTCTTC(1/4)
Eco81I 841 CCTGAGG CC^T[ACGT]AGG
EcoO109I 746 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 739 GGTCACC G^GT[ACGT]ACC
KasI 40 GGCGCC G^GCGCC
Ksp632I 957 GAAGAG CTCTTC(1/4)
NaeI 674 GCCGGC GCC^GGC
NarI 40 GGCGCC GG^CGCC
NgoMIV 674 GCCGGC G^CCGGC
PleI 295 GAGTC GAGTC(4/5)
RcaI 107 TCATGA T^CATGA
SapI 957 GAAGAGC GCTCTTC(1/4)
ScaI 881 AGTACT AGT^ACT
SfoI 40 GGCGCC GGC^GCC
SmlI 592 CTCAAG C^T[CT][GA]AG
StuI 845 AGGCCT AGG^CCT
TfiI 319 GAATC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AspHI 285 GTGCTC G[AT]GC[AT]^C
517 GTGCTC
BbrPI 325 CACGTG CAC^GTG
943 CACGTG
BsaAI 325 CACGTG [CT]AC^GT[GA]
943 CACGTG
BsgI 60 CTGCAC GTGCAG(16/14)
484 GTGCAG
BsiEI 299 CGGCCG CG[GA][CT]^CG
479 CGGCCG
BsiHKAI 285 GTGCTC G[AT]GC[AT]^C
517 GTGCTC
BsmAI 247 GAGAC GTCTC(1/5)
100 GTCTC
BssSI 679 CACGAG CACGAG(-5/-1)
1036 CACGAG
BstF5I 789 CATCC GGATG(2/0)
858 CATCC
BstYI 173 AGATCC [GA]^GATC[CT]
731 GGATCT
EagI 299 CGGCCG C^GGCCG
479 CGGCCG
EclXI 299 CGGCCG C^GGCCG
479 CGGCCG
Eco52I 299 CGGCCG C^GGCCG
479 CGGCCG
EcoT14I 46 CCAAGG C^C[AT][AT]GG
137 CCAAGG
FokI 789 CATCC GGATG(9/13)
858 CATCC
HinfI 295 GAGTC G^A[ACGT]TC
319 GAATC
KspI 819 CCGCGG CCGC^GG
913 CCGCGG
MaeIII 740 GTCAC ^GT[ACGT]AC
826 GTCAC
MflI 173 AGATCC [GA]^GATC[CT]
731 GGATCT
MslI 542 CACTCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1044 CATGAAAATG
NspI 698 GCATGC [GA]CATG^[CT]
1011 GCATGC
PflMI 324 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
857 CCATCCCCTGG
PmaCI 325 CACGTG CAC^GTG
943 CACGTG
PmlI 325 CACGTG CAC^GTG
943 CACGTG
PvuII 754 CAGCTG CAG^CTG
1006 CAGCTG
SacII 819 CCGCGG CCGC^GG
913 CCGCGG
SphI 698 GCATGC GCATG^C
1011 GCATGC
StyI 46 CCAAGG C^C[AT][AT]GG
137 CCAAGG
TaqI 668 TCGA T^CGA
977 TCGA
Tsp45I 740 GTCAC ^GT[GC]AC
826 GTCAC
TspRI 924 CAGTG CA[GC]TG[ACGT][ACGT]^
995 CACTG
TthHB8I 668 TCGA T^CGA
977 TCGA
Van91I 324 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
857 CCATCCCCTGG
XcmI 788 CCATCCGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
989 CCAGGGCACTGCTGG
XhoII 173 AGATCC [GA]^GATC[CT]
731 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TseI