##################################
# NO CUT ENZYMES (  181 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrGI       BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DdeI        DpnII       DraI        DraIII      
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
Hin1I       HincII      HindII      HinfI       HpaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MamI        MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MunI        NarI        NdeI        
NdeII       NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tru9I       Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccII          91  CGCG                 CG^CG
AcsI          152  GAATTC               [GA]^AATT[CT]
AluI          249  AGCT                 AG^CT
AlwNI         139  CAGACACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          152  GAATTC               [GA]^AATT[CT]
AspHI         312  GAGCAC               G[AT]GC[AT]^C
BmyI          312  GAGCAC               G[AGT]GC[ACT]^C
BsiHKAI       312  GAGCAC               G[AT]GC[AT]^C
BsmAI         254  GTCTC                GTCTC(1/5)
BsmFI         189  GTCCC                GGGAC(10/14)
Bsp1286I      312  GAGCAC               G[AGT]GC[ACT]^C
BsrFI          86  GCCGGC               [GA]^CCGG[CT]
BsrI          129  CCAGT                ACTGG(1/-1)
BssHII         90  GCGCGC               G^CGCGC
BstAPI        138  GCAGACACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI          72  CCTGG                CC^[AT]GG
BstUI          91  CGCG                 CG^CG
Cfr10I         86  GCCGGC               [GA]^CCGG[CT]
DraII         187  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EaeI           77  TGGCCG               [CT]^GGCC[GA]
EcoO109I      187  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         152  GAATTC               G^AATTC
EcoRII         72  CCTGG                ^CC[AT]GG
EcoT14I       272  CCATGG               C^C[AT][AT]GG
HaeII          54  GGCGCT               [GA]GCGC^[CT]
HgaI          103  GCGTC                GACGC(5/10)
HindIII       248  AAGCTT               A^AGCTT
HphI          156  TCACC                GGTGA(8/7)
MaeIII        112  GTCAC                ^GT[ACGT]AC
MboII         243  GAAGA                GAAGA(8/7)
MnlI          159  CCTC                 CCTC(7/6)
MvaI           72  CCTGG                CC^[AT]GG
MvnI           91  CGCG                 CG^CG
NaeI           86  GCCGGC               GCC^GGC
NcoI          272  CCATGG               C^CATGG
NgoMIV         86  GCCGGC               G^CCGGC
NlaIII        273  CATG                 CATG^
PpuMI         187  AGGTCCC              [GA]G^G[AT]CC[CT]
PstI          145  CTGCAG               CTGCA^G
SfaNI         265  GCATC                GCATC(5/9)
SfcI          145  CTGCAG               C^T[GA][CT]AG
StyI          272  CCATGG               C^C[AT][AT]GG
TaqI          290  TCGA                 T^CGA
Tsp45I        112  GTCAC                ^GT[GC]AC
Tsp509I       153  AATT                 ^AATT
TthHB8I       290  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AvaII         188  GGTCC                G^G[AT]CC
              196  GGACC               
BcnI           38  CCCGG                CC^[GC]GG
              193  CCCGG               
BstF5I        168  CATCC                GGATG(2/0)
              266  CATCC               
DsaI          107  CCGTGG               C^C[GA][CT]GG
              272  CCATGG              
FauI          262  CCCGC                CCCGC(4/6)
               48  GCGGG               
FokI          168  CATCC                GGATG(9/13)
              266  CATCC               
HaeIII          3  GGCC                 GG^CC
               78  GGCC                
NciI           38  CCCGG                CC^[GC]GG
              193  CCCGG               
SmlI          160  CTCAAG               C^T[CT][GA]AG
              232  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AciI        BbvI        BsaJI       BsiYI       BslI        
BssKI       Cac8I       CfoI        Cfr13I      Fnu4HI      
HapII       HhaI        HinP1I      HpaII       ItaI        
MspI        MwoI        NlaIV       Sau96I      ScrFI       
TseI        TspRI