Restriction map for
pFN21AB5918
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbsI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BpuAI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FspI HindIII HpaI KpnI
Ksp632I KspI MfeI MluI MroI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 24 CGCG CG^CG
AcsI 1233 AAATTC [GA]^AATT[CT]
AcyI 933 GGCGCC G[GA]^CG[CT]C
AflIII 865 ACGTGT A^C[GA][CT]GT
ApoI 1233 AAATTC [GA]^AATT[CT]
BalI 1225 TGGCCA TGG^CCA
BbeI 933 GGCGCC GGCGC^C
BcgI 509 CGACCACGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 579 GCCCCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1347 AGATCT A^GATCT
BsaAI 1497 CACGTA [CT]AC^GT[GA]
BsaBI 1440 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 933 GGCGCC G[GA]^CG[CT]C
BsaWI 224 ACCGGA [AT]^CCGG[AT]
BseRI 525 GAGGAG GAGGAG(10/8)
BsgI 869 GTGCAG GTGCAG(16/14)
BspMI 572 ACCTGC ACCTGC(4/8)
BsrFI 1132 GCCGGC [GA]^CCGG[CT]
BssSI 552 CTCGTG CACGAG(-5/-1)
BstUI 24 CGCG CG^CG
BstYI 1347 AGATCT [GA]^GATC[CT]
Cfr10I 1132 GCCGGC [GA]^CCGG[CT]
DrdI 84 GACATCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1068 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 1131 GGCCGGCC GGCCGG^CC
HaeII 933 GGCGCC [GA]GCGC^[CT]
HgaI 1020 GACGC GACGC(5/10)
Hin1I 933 GGCGCC G[GA]^CG[CT]C
HincII 300 GTCAAC GT[CT]^[GA]AC
HindII 300 GTCAAC GT[CT]^[GA]AC
KasI 933 GGCGCC G^GCGCC
MamI 1440 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1347 AGATCT [GA]^GATC[CT]
MluNI 1225 TGGCCA TGG^CCA
MscI 1225 TGGCCA TGG^CCA
MvnI 24 CGCG CG^CG
NaeI 1132 GCCGGC GCC^GGC
NarI 933 GGCGCC GG^CGCC
NcoI 537 CCATGG C^CATGG
NgoMIV 1132 GCCGGC G^CCGGC
NspI 139 ACATGC [GA]CATG^[CT]
PaeR7I 1343 CTCGAG C^TCGAG
PvuII 482 CAGCTG CAG^CTG
SacI 1282 GAGCTC GAGCT^C
SfoI 933 GGCGCC GGC^GCC
TaqI 1344 TCGA T^CGA
TthHB8I 1344 TCGA T^CGA
XhoI 1343 CTCGAG C^TCGAG
XhoII 1347 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 327 GTAC GT^AC
1500 GTAC
ApaI 1050 GGGCCC GGGCC^C
1058 GGGCCC
BbvCI 1491 CCTCAGC CCTCAGC(-5/-2)
499 GCTGAGG
BfaI 679 CTAG C^TAG
1007 CTAG
BsaI 181 GAGACC GGTCTC(1/5)
963 GGTCTC
BstEII 1395 GGTCACC G^GT[ACGT]ACC
1407 GGTGACC
BstF5I 108 CATCC GGATG(2/0)
1299 GGATG
BstPI 1395 GGTCACC G^GT[ACGT]ACC
1407 GGTGACC
BstXI 164 CCAAGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
512 CCACGGTGCTGG
EcoO65I 1395 GGTCACC G^GT[ACGT]ACC
1407 GGTGACC
FokI 108 CATCC GGATG(9/13)
1299 GGATG
MaeI 679 CTAG C^TAG
1007 CTAG
MboII 256 GAAGA GAAGA(8/7)
1521 GAAGA
MslI 438 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1449 CATCACTGTG
PshAI 630 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
699 GACCGTGGTC
PstI 904 CTGCAG CTGCA^G
1484 CTGCAG
RsaI 327 GTAC GT^AC
1500 GTAC
StuI 818 AGGCCT AGG^CCT
1488 AGGCCT
TfiI 945 GATTC G^A[AT]TC
1327 GAATC
Tsp509I 547 AATT ^AATT
1234 AATT
XcmI 405 CCAGGTGGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
431 CCAACCTCACCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
Sau3AI Sau96I ScrFI SfcI SmaI
SmlI StyI TseI Tsp45I TspRI
Van91I XmaCI XmaI