Restriction map for
pFN21AE1341
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AfaI AgeI AhdI AluI
AlwI AlwNI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanII BbrPI BbsI BbvCI BcgI
BciVI BfaI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstXI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HgaI HindIII HinfI
HpaI KpnI KspI MaeI MamI
MfeI MflI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SfaNI SfcI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI TspRI Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcsI 82 GAATTT [GA]^AATT[CT]
AcyI 34 GGCGCC G[GA]^CG[CT]C
AflII 268 CTTAAG C^TTAAG
AflIII 47 ACGTGT A^C[GA][CT]GT
Alw44I 466 GTGCAC G^TGCAC
AocI 283 CCTGAGG CC^T[ACGT]AGG
ApaLI 466 GTGCAC G^TGCAC
ApoI 82 GAATTT [GA]^AATT[CT]
Asp700I 289 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 466 GTGCAC G[AT]GC[AT]^C
BbeI 34 GGCGCC GGCGC^C
BbvI 160 GCTGC GCAGC(8/12)
BclI 206 TGATCA T^GATCA
BfrI 268 CTTAAG C^TTAAG
BmyI 466 GTGCAC G[AGT]GC[ACT]^C
BsaHI 34 GGCGCC G[GA]^CG[CT]C
BseRI 397 GAGGAG GAGGAG(10/8)
BsiHKAI 466 GTGCAC G[AT]GC[AT]^C
BsmAI 149 GAGAC GTCTC(1/5)
BsmI 304 GCATTC GAATGC(1/-1)
Bsp1286I 466 GTGCAC G[AGT]GC[ACT]^C
BsrBI 275 GAGCGG CCGCTC(-3/-3)
BsrI 439 ACTGG ACTGG(1/-1)
BstBI 61 TTCGAA TT^CGAA
BstF5I 120 GGATG GGATG(2/0)
Bsu36I 283 CCTGAGG CC^T[ACGT]AGG
Cac8I 375 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 35 GCGC GCG^C
DsaI 480 CCACGG C^C[GA][CT]GG
Eco81I 283 CCTGAGG CC^T[ACGT]AGG
FbaI 206 TGATCA T^GATCA
FokI 120 GGATG GGATG(9/13)
HaeII 34 GGCGCC [GA]GCGC^[CT]
HaeIII 93 GGCC GG^CC
HhaI 35 GCGC GCG^C
Hin1I 34 GGCGCC G[GA]^CG[CT]C
HinP1I 35 GCGC G^CGC
HincII 403 GTTGAC GT[CT]^[GA]AC
HindII 403 GTTGAC GT[CT]^[GA]AC
HphI 476 TCACC GGTGA(8/7)
KasI 34 GGCGCC G^GCGCC
MaeIII 436 GTGAC ^GT[ACGT]AC
MslI 99 CACTATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 34 GGCGCC GG^CGCC
NspV 61 TTCGAA TT^CGAA
SexAI 461 ACCTGGT A^CC[AT]GGT
SfoI 34 GGCGCC GGC^GCC
SfuI 61 TTCGAA TT^CGAA
SmlI 268 CTTAAG C^T[CT][GA]AG
StuI 92 AGGCCT AGG^CCT
TaqI 62 TCGA T^CGA
TseI 160 GCTGC G^C[AT]GC
Tsp45I 436 GTGAC ^GT[GC]AC
TthHB8I 62 TCGA T^CGA
XcmI 111 CCAGAACAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 289 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BanI 3 GGCACC G^G[CT][GA]CC
34 GGCGCC
BcnI 29 CCGGG CC^[GC]GG
220 CCGGG
BsiYI 72 CCAAATCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
111 CCAGAACAGGG
BslI 72 CCAAATCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
111 CCAGAACAGGG
BstNI 78 CCAGG CC^[AT]GG
462 CCTGG
DpnII 142 GATC ^GATC
207 GATC
EarI 21 GAAGAG CTCTTC(1/4)
487 GAAGAG
EcoRII 78 CCAGG ^CC[AT]GG
462 CCTGG
EcoT14I 165 CCTTGG C^C[AT][AT]GG
392 CCAAGG
Fnu4HI 160 GCTGC GC^[ACGT]GC
418 GCCGC
HapII 29 CCGG C^CGG
220 CCGG
HpaII 29 CCGG C^CGG
220 CCGG
ItaI 160 GCTGC GC^[ACGT]GC
418 GCCGC
Ksp632I 21 GAAGAG CTCTTC(1/4)
487 GAAGAG
MboI 142 GATC ^GATC
207 GATC
MspI 29 CCGG C^CGG
220 CCGG
MvaI 78 CCAGG CC^[AT]GG
462 CCTGG
MwoI 28 GCCGGGGGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
366 GCTGACCACGC
NciI 29 CCGGG CC^[GC]GG
220 CCGGG
NdeII 142 GATC ^GATC
207 GATC
NlaIII 57 CATG CATG^
105 CATG
Sau3AI 142 GATC ^GATC
207 GATC
StyI 165 CCTTGG C^C[AT][AT]GG
392 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AciI AvaII BsaJI BssKI Cfr13I
DdeI MaeII MboII MnlI MseI
NlaIV Sau96I ScrFI Tru9I Tsp509I