Restriction map for
pFN21AE4596
##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BbeI BcgI
BclI BfrI BlnI BlpI Bpu1102I
BsiEI BsiWI BsmBI BspDI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KasI KpnI
MfeI MluI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SbfI ScaI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI Tru9I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 2416 GTCGAC GT^[AC][GT]AC
AccIII 1170 TCCGGA T^CCGGA
AcyI 862 GACGCC G[GA]^CG[CT]C
Alw44I 1900 GTGCAC G^TGCAC
Aor51HI 1814 AGCGCT AGC^GCT
ApaLI 1900 GTGCAC G^TGCAC
AviII 2042 TGCGCA TGC^GCA
BbrPI 1688 CACGTG CAC^GTG
BbvCI 517 GCTGAGG CCTCAGC(-5/-2)
BfaI 778 CTAG C^TAG
BsaBI 883 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 862 GACGCC G[GA]^CG[CT]C
BsaWI 1170 TCCGGA [AT]^CCGG[AT]
BseAI 1170 TCCGGA T^CCGGA
BspEI 1170 TCCGGA T^CCGGA
BspHI 1190 TCATGA T^CATGA
BspLU11I 1868 ACATGT A^CATGT
BsrBI 3112 GAGCGG CCGCTC(-3/-3)
BsrFI 404 GCCGGC [GA]^CCGG[CT]
BssSI 2701 CTCGTG CACGAG(-5/-1)
Cfr10I 404 GCCGGC [GA]^CCGG[CT]
DraIII 2743 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1814 AGCGCT AGC^GCT
FspI 2042 TGCGCA TGC^GCA
Hin1I 862 GACGCC G[GA]^CG[CT]C
HincII 2416 GTCGAC GT[CT]^[GA]AC
HindII 2416 GTCGAC GT[CT]^[GA]AC
KspI 1650 CCGCGG CCGC^GG
MaeI 778 CTAG C^TAG
MamI 883 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1170 TCCGGA T^CCGGA
NaeI 404 GCCGGC GCC^GGC
NgoMIV 404 GCCGGC G^CCGGC
PmaCI 1688 CACGTG CAC^GTG
PmlI 1688 CACGTG CAC^GTG
RcaI 1190 TCATGA T^CATGA
SacII 1650 CCGCGG CCGC^GG
SalI 2416 GTCGAC G^TCGAC
SexAI 561 ACCTGGT A^CC[AT]GGT
SfiI 407 GGCCTCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3536 GCATGC GCATG^C
SspI 2429 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 1651 CGCG CG^CG
1888 CGCG
AspI 976 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2947 GACCCTGTC
BamHI 255 GGATCC G^GATCC
2862 GGATCC
BbsI 249 GAAGAC GAAGAC(2/6)
772 GAAGAC
BciVI 453 GTATCC GTATCC(6/5)
805 GTATCC
BpuAI 249 GAAGAC GAAGAC(2/6)
772 GAAGAC
BsmI 3089 GAATGC GAATGC(1/-1)
3596 GCATTC
BsrDI 1035 CATTGC GCAATG(2/0)
3143 CATTGC
BstUI 1651 CGCG CG^CG
1888 CGCG
EcoNI 1962 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3390 CCTCAAGCAGG
HaeII 1814 AGCGCT [GA]GCGC^[CT]
3602 AGCGCC
MvnI 1651 CGCG CG^CG
1888 CGCG
NcoI 132 CCATGG C^CATGG
1997 CCATGG
PflFI 976 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2947 GACCCTGTC
SapI 1788 GAAGAGC GCTCTTC(1/4)
1947 GCTCTTC
SmaI 1724 CCCGGG CCC^GGG
3530 CCCGGG
Tth111I 976 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2947 GACCCTGTC
XmaCI 1724 CCCGGG C^CCGGG
3530 CCCGGG
XmaI 1724 CCCGGG C^CCGGG
3530 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BglI BglII
BmyI BpmI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII