Restriction map for
pFN21AE1454
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII BbeI BcgI BciVI BfrI
BglII BlpI Bpu1102I BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
TfiI Tru9I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AatII 1457 GACGTC GACGT^C
AccII 535 CGCG CG^CG
Alw44I 896 GTGCAC G^TGCAC
ApaLI 896 GTGCAC G^TGCAC
AspI 970 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1940 TGGCCA TGG^CCA
BbrPI 58 CACGTG CAC^GTG
BbsI 1663 GAAGAC GAAGAC(2/6)
BbvCI 1163 CCTCAGC CCTCAGC(-5/-2)
BclI 113 TGATCA T^GATCA
BglI 1933 GCCACTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1663 GAAGAC GAAGAC(2/6)
BsaAI 58 CACGTG [CT]AC^GT[GA]
BsaBI 1386 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BssSI 1249 CTCGTG CACGAG(-5/-1)
BstUI 535 CGCG CG^CG
DrdI 1451 GACATCGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 113 TGATCA T^GATCA
HgaI 1101 GCGTC GACGC(5/10)
MamI 1386 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1940 TGGCCA TGG^CCA
MscI 1940 TGGCCA TGG^CCA
MvnI 535 CGCG CG^CG
NspI 273 ACATGC [GA]CATG^[CT]
PflFI 970 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 58 CACGTG CAC^GTG
PmlI 58 CACGTG CAC^GTG
SbfI 1510 CCTGCAGG CCTGCA^GG
Sse8387I 1510 CCTGCAGG CCTGCA^GG
StuI 1545 AGGCCT AGG^CCT
Tth111I 970 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 1141 GAATTC [GA]^AATT[CT]
1750 GAATTC
AcyI 1100 GGCGTC G[GA]^CG[CT]C
1457 GACGTC
AlwNI 104 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1514 CAGGGGCTG
ApoI 1141 GAATTC [GA]^AATT[CT]
1750 GAATTC
AvaI 31 CTCGGG C^[CT]CG[GA]G
564 CCCGAG
AvrII 657 CCTAGG C^CTAGG
1708 CCTAGG
BamHI 215 GGATCC G^GATCC
1347 GGATCC
BanI 71 GGCACC G^G[CT][GA]CC
1370 GGCACC
BfaI 658 CTAG C^TAG
1709 CTAG
BlnI 657 CCTAGG C^CTAGG
1708 CCTAGG
BsaHI 1100 GGCGTC G[GA]^CG[CT]C
1457 GACGTC
BsmFI 1177 GGGAC GGGAC(10/14)
531 GTCCC
BsoBI 31 CTCGGG C^[CT]CG[GA]G
564 CCCGAG
BspMI 1217 ACCTGC ACCTGC(4/8)
572 GCAGGT
BsrBI 318 CCGCTC CCGCTC(-3/-3)
864 CCGCTC
BstXI 392 CCAGCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
516 CCATGCCCGTGG
BstYI 215 GGATCC [GA]^GATC[CT]
1347 GGATCC
CfoI 536 GCGC GCG^C
809 GCGC
EaeI 1400 TGGCCG [CT]^GGCC[GA]
1940 TGGCCA
EarI 1435 CTCTTC CTCTTC(1/4)
1780 GAAGAG
EcoRI 1141 GAATTC G^AATTC
1750 GAATTC
FauI 533 CCCGC CCCGC(4/6)
1174 GCGGG
HhaI 536 GCGC GCG^C
809 GCGC
Hin1I 1100 GGCGTC G[GA]^CG[CT]C
1457 GACGTC
HinP1I 536 GCGC G^CGC
809 GCGC
Ksp632I 1435 CTCTTC CTCTTC(1/4)
1780 GAAGAG
MaeI 658 CTAG C^TAG
1709 CTAG
MflI 215 GGATCC [GA]^GATC[CT]
1347 GGATCC
NcoI 1096 CCATGG C^CATGG
1148 CCATGG
Tsp509I 1142 AATT ^AATT
1751 AATT
XhoII 215 GGATCC [GA]^GATC[CT]
1347 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwI AocI
ApaI AspHI AvaII BanII BbvI
BcnI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII DsaI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XcmI