Restriction map for
pFN21AE3654
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BanI BanII BbeI
BbrPI BbvCI BciVI BclI BfaI
BfrI BglI BglII BlnI BmyI
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MroI MseI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StyI SwaI TaqI Tru9I Tsp45I
Tsp509I Tth111I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 676 GTCTAC GT^[AC][GT]AC
AfaI 561 GTAC GT^AC
AflIII 536 ACATGT A^C[GA][CT]GT
AvaI 123 CTCGGG C^[CT]CG[GA]G
BalI 519 TGGCCA TGG^CCA
BamHI 707 GGATCC G^GATCC
BbsI 208 GTCTTC GAAGAC(2/6)
BcgI 222 GCATAACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 270 CCGGG CC^[GC]GG
BlpI 392 GCTCAGC GC^T[ACGT]AGC
BpmI 379 CTGGAG CTGGAG(16/14)
Bpu1102I 392 GCTCAGC GC^T[ACGT]AGC
BpuAI 208 GTCTTC GAAGAC(2/6)
BsaI 611 GAGACC GGTCTC(1/5)
BsmAI 611 GAGAC GTCTC(1/5)
BsoBI 123 CTCGGG C^[CT]CG[GA]G
BspLU11I 536 ACATGT A^CATGT
BstYI 707 GGATCC [GA]^GATC[CT]
CelII 392 GCTCAGC GC^T[ACGT]AGC
CfoI 330 GCGC GCG^C
DraIII 481 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 519 TGGCCA [CT]^GGCC[GA]
HapII 270 CCGG C^CGG
HhaI 330 GCGC GCG^C
HinP1I 330 GCGC G^CGC
HpaII 270 CCGG C^CGG
MaeII 686 ACGT A^CGT
MaeIII 641 GTAAC ^GT[ACGT]AC
MflI 707 GGATCC [GA]^GATC[CT]
MluNI 519 TGGCCA TGG^CCA
MscI 519 TGGCCA TGG^CCA
MspI 270 CCGG C^CGG
NciI 270 CCGGG CC^[GC]GG
PflMI 414 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 561 GTAC GT^AC
SexAI 482 ACCTGGT A^CC[AT]GGT
SfaNI 295 GATGC GCATC(5/9)
SphI 507 GCATGC GCATG^C
StuI 624 AGGCCT AGG^CCT
Van91I 414 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 707 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BseRI 49 GAGGAG GAGGAG(10/8)
496 GAGGAG
BsmFI 386 GGGAC GGGAC(10/14)
489 GTCCC
BsrI 704 ACTGG ACTGG(1/-1)
465 CCAGT
DdeI 22 CTGAG C^T[ACGT]AG
393 CTCAG
DraII 146 GGGTCCT [GA]G^G[ACGT]CC[CT]
167 GGGTCCT
EcoO109I 146 GGGTCCT [GA]G^G[ACGT]CC[CT]
167 GGGTCCT
NspI 507 GCATGC [GA]CATG^[CT]
536 ACATGT
PleI 24 GAGTC GAGTC(4/5)
674 GAGTC
PpuMI 146 GGGTCCT [GA]G^G[AT]CC[CT]
167 GGGTCCT
PstI 35 CTGCAG CTGCA^G
690 CTGCAG
PvuII 176 CAGCTG CAG^CTG
408 CAGCTG
SfcI 35 CTGCAG C^T[GA][CT]AG
690 CTGCAG
XcmI 129 CCACTTGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
579 CCAGGGTGATTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BsaJI BsiYI BslI BssKI BstF5I
BstNI Cac8I Cfr13I DpnII EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MboI MboII MnlI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TfiI TseI
TspRI