Restriction map for
pFN21AE1324
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# NO CUT ENZYMES ( 100 enzymes) #
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AatII AccI AccIII AclI AflII
AflIII AgeI Aor51HI AscI AseI
AsnI Asp700I AviII BamHI BbeI
BbrPI BbsI BciVI BfrI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspEI BspHI BspLU11I BsrDI BssHII
Bst1107I BstZ17I CelII CpoI DraI
EagI EclXI Eco47III Eco52I EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KasI KspI MaeII
MamI MfeI MluI MroI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NsiI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SgrAI SmaI
SnaBI Sse8387I SspI SwaI Tru9I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 1471 GAATTC [GA]^AATT[CT]
AcyI 570 GACGCC G[GA]^CG[CT]C
AhdI 1988 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 204 CCTGAGG CC^T[ACGT]AGG
ApoI 1471 GAATTC [GA]^AATT[CT]
AspEI 1988 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1515 CCTAGG C^CTAGG
BalI 947 TGGCCA TGG^CCA
BcgI 1972 CGAAGCAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 663 TGATCA T^GATCA
BglII 510 AGATCT A^GATCT
BlnI 1515 CCTAGG C^CTAGG
BsaHI 570 GACGCC G[GA]^CG[CT]C
BsaI 631 GAGACC GGTCTC(1/5)
Bsp1407I 558 TGTACA T^GTACA
BspDI 1931 ATCGAT AT^CGAT
BsrBI 64 CCGCTC CCGCTC(-3/-3)
BsrFI 2336 GCCGGT [GA]^CCGG[CT]
BsrGI 558 TGTACA T^GTACA
BssSI 2264 CACGAG CACGAG(-5/-1)
BstAPI 1375 GCACAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1231 TTCGAA TT^CGAA
BstEII 2350 GGTGACC G^GT[ACGT]ACC
BstPI 2350 GGTGACC G^GT[ACGT]ACC
Bsu36I 204 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2336 GCCGGT [GA]^CCGG[CT]
ClaI 1931 ATCGAT AT^CGAT
DraIII 1376 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1394 GACCCTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1983 CCATGG C^C[GA][CT]GG
Eam1105I 1988 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 204 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2350 GGTGACC G^GT[ACGT]ACC
EcoRI 1471 GAATTC G^AATTC
FbaI 663 TGATCA T^GATCA
Hin1I 570 GACGCC G[GA]^CG[CT]C
MluNI 947 TGGCCA TGG^CCA
MscI 947 TGGCCA TGG^CCA
NcoI 1983 CCATGG C^CATGG
NruI 499 TCGCGA TCG^CGA
NspI 1556 GCATGC [GA]CATG^[CT]
NspV 1231 TTCGAA TT^CGAA
SexAI 806 ACCTGGT A^CC[AT]GGT
SfuI 1231 TTCGAA TT^CGAA
SmlI 2182 CTCAAG C^T[CT][GA]AG
SpeI 774 ACTAGT A^CTAGT
SphI 1556 GCATGC GCATG^C
SspBI 558 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AccII 39 CGCG CG^CG
500 CGCG
Alw44I 1373 GTGCAC G^TGCAC
1843 GTGCAC
ApaLI 1373 GTGCAC G^TGCAC
1843 GTGCAC
AspI 1324 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2421 GACAGTGTC
BbvCI 2097 CCTCAGC CCTCAGC(-5/-2)
1362 GCTGAGG
BstUI 39 CGCG CG^CG
500 CGCG
EarI 1228 CTCTTC CTCTTC(1/4)
362 GAAGAG
HgaI 570 GACGC GACGC(5/10)
1328 GCGTC
Ksp632I 1228 CTCTTC CTCTTC(1/4)
362 GAAGAG
MvnI 39 CGCG CG^CG
500 CGCG
PflFI 1324 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2421 GACAGTGTC
PstI 1176 CTGCAG CTGCA^G
1833 CTGCAG
Tth111I 1324 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2421 GACAGTGTC
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
Acc65I AciI AfaI AluI AlwI
AlwNI ApaI Asp718I AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI KpnI
MaeI MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII