Restriction map for
pFN21AE1513
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AvaI AviII
AvrII BanI BanII BbeI BbrPI
BbsI BcgI BclI BfaI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT14I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaI KasI KpnI KspI MaeI
MaeII MamI MluI MroI MslI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccII 113 CGCG CG^CG
AciI 363 CCGC CCGC(-3/-1)
AfaI 131 GTAC GT^AC
AhdI 58 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 18 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 58 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 323 GGACC G^G[AT]CC
BalI 35 TGGCCA TGG^CCA
BamHI 243 GGATCC G^GATCC
BbvCI 157 GCTGAGG CCTCAGC(-5/-2)
BbvI 23 GCTGC GCAGC(8/12)
BciVI 359 GTATCC GTATCC(6/5)
BcnI 13 CCCGG CC^[GC]GG
BglI 365 GCCTTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 92 GCTCAGC GC^T[ACGT]AGC
BmyI 29 GTGCCC G[AGT]GC[ACT]^C
BpmI 194 CTGGAG CTGGAG(16/14)
Bpu1102I 92 GCTCAGC GC^T[ACGT]AGC
BsaI 211 GAGACC GGTCTC(1/5)
BsmFI 322 GGGAC GGGAC(10/14)
Bsp1286I 29 GTGCCC G[AGT]GC[ACT]^C
BstAPI 17 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 513 GGTCACC G^GT[ACGT]ACC
BstPI 513 GGTCACC G^GT[ACGT]ACC
BstUI 113 CGCG CG^CG
BstYI 243 GGATCC [GA]^GATC[CT]
Cac8I 45 GCTGGC GC[ACGT]^[ACGT]GC
CelII 92 GCTCAGC GC^T[ACGT]AGC
DpnII 244 GATC ^GATC
DraI 219 TTTAAA TTT^AAA
DraII 322 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 35 TGGCCA [CT]^GGCC[GA]
Eam1105I 58 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 151 GAAGAG CTCTTC(1/4)
EcoO109I 322 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 513 GGTCACC G^GT[ACGT]ACC
EcoT22I 75 ATGCAT ATGCA^T
Fnu4HI 23 GCTGC GC^[ACGT]GC
HapII 14 CCGG C^CGG
HindIII 536 AAGCTT A^AGCTT
HpaII 14 CCGG C^CGG
ItaI 23 GCTGC GC^[ACGT]GC
Ksp632I 151 GAAGAG CTCTTC(1/4)
MboI 244 GATC ^GATC
MboII 151 GAAGA GAAGA(8/7)
MfeI 50 CAATTG C^AATTG
MflI 243 GGATCC [GA]^GATC[CT]
MluNI 35 TGGCCA TGG^CCA
MscI 35 TGGCCA TGG^CCA
MspA1I 226 CAGCTG C[AC]G^C[GT]G
MspI 14 CCGG C^CGG
MunI 50 CAATTG C^AATTG
MvnI 113 CGCG CG^CG
NciI 13 CCCGG CC^[GC]GG
NdeII 244 GATC ^GATC
NsiI 75 ATGCAT ATGCA^T
PpuMI 322 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 226 CAGCTG CAG^CTG
RsaI 131 GTAC GT^AC
Sau3AI 244 GATC ^GATC
SfaNI 74 GATGC GCATC(5/9)
SmlI 445 CTTGAG C^T[CT][GA]AG
TseI 23 GCTGC G^C[AT]GC
Tsp509I 51 AATT ^AATT
XhoII 243 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 3 enzymes) #
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AlwI 244 GATCC GGATC(4/5)
243 GGATC
BsiYI 13 CCCGGCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
363 CCGCCTTCAGG
BslI 13 CCCGGCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
363 CCGCCTTCAGG
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AluI BsaJI BsmAI BsrI BssKI
BstF5I BstNI BstXI Cfr13I DdeI
EcoRII FokI HaeIII HphI MaeIII
MnlI MseI MvaI MwoI NlaIV
Sau96I ScrFI Tru9I Tsp45I TspRI