Restriction map for
pFN21AE9277
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HaeII Hin1I HincII HindII HinfI
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MroI
MseI MunI MvnI MwoI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 457 GTAGAC GT^[AC][GT]AC
AcsI 49 AAATTC [GA]^AATT[CT]
AlwI 489 GATCC GGATC(4/5)
AlwNI 23 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 49 AAATTC [GA]^AATT[CT]
AspHI 433 GAGCAC G[AT]GC[AT]^C
AvaI 532 CTCGGG C^[CT]CG[GA]G
BcnI 408 CCCGG CC^[GC]GG
BmyI 433 GAGCAC G[AGT]GC[ACT]^C
BsaI 529 GGTCTC GGTCTC(1/5)
BseRI 302 CTCCTC GAGGAG(10/8)
BsiHKAI 433 GAGCAC G[AT]GC[AT]^C
BsmAI 530 GTCTC GTCTC(1/5)
BsoBI 532 CTCGGG C^[CT]CG[GA]G
Bsp1286I 433 GAGCAC G[AGT]GC[ACT]^C
BsrBI 243 CCGCTC CCGCTC(-3/-3)
BstYI 488 AGATCC [GA]^GATC[CT]
CfoI 448 GCGC GCG^C
DdeI 158 CTGAG C^T[ACGT]AG
DrdI 190 GACATTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 539 CCATGG C^C[GA][CT]GG
EcoT14I 539 CCATGG C^C[AT][AT]GG
FauI 372 GCGGG CCCGC(4/6)
HapII 409 CCGG C^CGG
HhaI 448 GCGC GCG^C
HinP1I 448 GCGC G^CGC
HindIII 115 AAGCTT A^AGCTT
HpaII 409 CCGG C^CGG
HphI 200 TCACC GGTGA(8/7)
MflI 488 AGATCC [GA]^GATC[CT]
MslI 420 CACCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 23 CAGCTG C[AC]G^C[GT]G
MspI 409 CCGG C^CGG
NciI 408 CCCGG CC^[GC]GG
NcoI 539 CCATGG C^CATGG
PvuII 23 CAGCTG CAG^CTG
SmlI 466 CTCAAG C^T[CT][GA]AG
StyI 539 CCATGG C^C[AT][AT]GG
TaqI 485 TCGA T^CGA
Tsp509I 50 AATT ^AATT
TthHB8I 485 TCGA T^CGA
XcmI 539 CCATGGCTGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 488 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaII 381 GGACC G^G[AT]CC
554 GGACC
BalI 332 TGGCCA TGG^CCA
500 TGGCCA
BsgI 29 CTGCAC GTGCAG(16/14)
259 CTGCAC
BsmFI 380 GGGAC GGGAC(10/14)
553 GGGAC
BsrI 253 CCAGT ACTGG(1/-1)
335 CCAGT
Cac8I 372 GCGGGC GC[ACGT]^[ACGT]GC
389 GCAAGC
DpnII 441 GATC ^GATC
489 GATC
HgaI 214 GACGC GACGC(5/10)
365 GCGTC
MboI 441 GATC ^GATC
489 GATC
MluNI 332 TGGCCA TGG^CCA
500 TGGCCA
MscI 332 TGGCCA TGG^CCA
500 TGGCCA
NdeII 441 GATC ^GATC
489 GATC
SapI 237 GAAGAGC GCTCTTC(1/4)
399 GCTCTTC
Sau3AI 441 GATC ^GATC
489 GATC
SfaNI 310 GATGC GCATC(5/9)
341 GCATC
SfcI 177 CTACAG C^T[GA][CT]AG
207 CTACAG
StuI 11 AGGCCT AGG^CCT
470 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BspMI BssKI BstNI BstXI
Cfr13I EaeI EarI EcoRII Fnu4HI
HaeIII ItaI Ksp632I MaeIII MboII
MnlI MvaI NlaIII NlaIV Sau96I
ScrFI TseI Tsp45I TspRI