Restriction map for
pFN21AE3636
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI BspDI BspEI
BspLU11I BspMI BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DpnII
DraI DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRII
EcoRV EcoT22I FauI FbaI FseI
FspI Hin1I HincII HindII HindIII
HinfI HpaI KasI KpnI Ksp632I
KspI MaeIII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MslI MspA1I MunI MvaI MvnI
NarI NdeI NdeII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PleI PmeI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tsp45I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 184 GAATTT [GA]^AATT[CT]
AfaI 243 GTAC GT^AC
AflIII 239 ACGTGT A^C[GA][CT]GT
ApoI 184 GAATTT [GA]^AATT[CT]
AspI 458 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 165 GGCACC G^G[CT][GA]CC
BbrPI 238 CACGTG CAC^GTG
BfaI 5 CTAG C^TAG
BpmI 103 CTGGAG CTGGAG(16/14)
BsaAI 238 CACGTG [CT]AC^GT[GA]
BsmI 75 GAATGC GAATGC(1/-1)
Bsp1407I 242 TGTACA T^GTACA
BspHI 113 TCATGA T^CATGA
BsrBI 106 GAGCGG CCGCTC(-3/-3)
BsrFI 220 GCCGGC [GA]^CCGG[CT]
BsrGI 242 TGTACA T^GTACA
Cfr10I 220 GCCGGC [GA]^CCGG[CT]
DdeI 299 CTGAG C^T[ACGT]AG
DraII 456 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 456 AGGACCC [GA]G^G[ACGT]CC[CT]
HgaI 57 GACGC GACGC(5/10)
HphI 539 TCACC GGTGA(8/7)
MaeI 5 CTAG C^TAG
MaeII 239 ACGT A^CGT
MseI 400 TTAA T^TAA
NaeI 220 GCCGGC GCC^GGC
NgoMIV 220 GCCGGC G^CCGGC
NheI 4 GCTAGC G^CTAGC
PflFI 458 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 238 CACGTG CAC^GTG
PmlI 238 CACGTG CAC^GTG
PpuMI 456 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 57 GACGCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 356 CTGCAG CTGCA^G
RcaI 113 TCATGA T^CATGA
RsaI 243 GTAC GT^AC
SfcI 356 CTGCAG C^T[GA][CT]AG
SmlI 97 CTCAAG C^T[CT][GA]AG
SspBI 242 TGTACA T^GTACA
TaqI 171 TCGA T^CGA
Tru9I 400 TTAA T^TAA
TspRI 225 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 458 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 171 TCGA T^CGA
XcmI 533 CCAGTTTCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaII 457 GGACC G^G[AT]CC
482 GGTCC
BbvI 488 GCAGC GCAGC(8/12)
355 GCTGC
BcnI 123 CCCGG CC^[GC]GG
460 CCCGG
BmyI 164 GGGCAC G[AGT]GC[ACT]^C
529 GTGCCC
Bsp1286I 164 GGGCAC G[AGT]GC[ACT]^C
529 GTGCCC
BssKI 123 CCCGG ^CC[ACGT]GG
460 CCCGG
BstF5I 66 CATCC GGATG(2/0)
288 GGATG
Cac8I 4 GCTAGC GC[ACGT]^[ACGT]GC
220 GCCGGC
DsaI 293 CCATGG C^C[GA][CT]GG
542 CCATGG
FokI 66 CATCC GGATG(9/13)
288 GGATG
HaeII 377 AGCGCC [GA]GCGC^[CT]
389 AGCGCC
HaeIII 38 GGCC GG^CC
219 GGCC
NciI 123 CCCGG CC^[GC]GG
460 CCCGG
NcoI 293 CCATGG C^CATGG
542 CCATGG
ScrFI 123 CCCGG CC^[ACGT]GG
460 CCCGG
SfaNI 289 GATGC GCATC(5/9)
502 GCATC
TseI 355 GCTGC G^C[AT]GC
488 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AluI BsaJI BseRI BsrI
CfoI Cfr13I EcoT14I Fnu4HI HapII
HhaI HinP1I HpaII ItaI MboII
MnlI MspI MwoI NlaIII NlaIV
Sau96I StyI Tsp509I