Restriction map for
pFN21AE1350
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# NO CUT ENZYMES ( 68 enzymes) #
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Acc65I AclI AflII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvrII BcgI
BciVI BclI BfrI BlnI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI Eam1105I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KpnI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SbfI SfiI SfuI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 2626 GACGTC GACGT^C
AccI 510 GTCGAC GT^[AC][GT]AC
AccIII 326 TCCGGA T^CCGGA
AcsI 3319 AAATTC [GA]^AATT[CT]
AflIII 1805 ACGTGT A^C[GA][CT]GT
Aor51HI 2681 AGCGCT AGC^GCT
ApoI 3319 AAATTC [GA]^AATT[CT]
Asp700I 705 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2852 TGCGCA TGC^GCA
BbrPI 1153 CACGTG CAC^GTG
BglI 2791 GCCCTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1332 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 326 TCCGGA [AT]^CCGG[AT]
BseAI 326 TCCGGA T^CCGGA
BsmI 2344 GAATGC GAATGC(1/-1)
BspEI 326 TCCGGA T^CCGGA
BssHII 3076 GCGCGC G^CGCGC
BstEII 3264 GGTGACC G^GT[ACGT]ACC
BstPI 3264 GGTGACC G^GT[ACGT]ACC
BstXI 2405 CCACCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 1280 CGGCCG C^GGCCG
EclXI 1280 CGGCCG C^GGCCG
Eco47III 2681 AGCGCT AGC^GCT
Eco52I 1280 CGGCCG C^GGCCG
EcoO65I 3264 GGTGACC G^GT[ACGT]ACC
FspI 2852 TGCGCA TGC^GCA
KspI 1105 CCGCGG CCGC^GG
MamI 1332 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 326 TCCGGA T^CCGGA
MseI 536 TTAA T^TAA
NspI 1487 GCATGC [GA]CATG^[CT]
PaeR7I 787 CTCGAG C^TCGAG
PmaCI 1153 CACGTG CAC^GTG
PmlI 1153 CACGTG CAC^GTG
SacI 3328 GAGCTC GAGCT^C
SacII 1105 CCGCGG CCGC^GG
SalI 510 GTCGAC G^TCGAC
SapI 3003 GCTCTTC GCTCTTC(1/4)
ScaI 3158 AGTACT AGT^ACT
SgrAI 2651 CACCGGCG C[GA]^CCGG[CT]G
SmaI 1226 CCCGGG CCC^GGG
SphI 1487 GCATGC GCATG^C
StuI 78 AGGCCT AGG^CCT
TfiI 345 GAATC G^A[AT]TC
Tru9I 536 TTAA T^TAA
Tsp509I 3320 AATT ^AATT
XcmI 62 CCATGGCTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 787 CTCGAG C^TCGAG
XmaCI 1226 CCCGGG C^CCGGG
XmaI 1226 CCCGGG C^CCGGG
XmnI 705 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
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AocI 837 CCTGAGG CC^T[ACGT]AGG
1846 CCTGAGG
ApaI 376 GGGCCC GGGCC^C
928 GGGCCC
BamHI 1349 GGATCC G^GATCC
2324 GGATCC
BbeI 2293 GGCGCC GGCGC^C
2401 GGCGCC
BlpI 1212 GCTGAGC GC^T[ACGT]AGC
1734 GCTCAGC
Bpu1102I 1212 GCTGAGC GC^T[ACGT]AGC
1734 GCTCAGC
BsaI 3065 GAGACC GGTCTC(1/5)
1992 GGTCTC
BsiEI 512 CGACCG CG[GA][CT]^CG
1280 CGGCCG
BspHI 1982 TCATGA T^CATGA
3440 TCATGA
BspMI 2966 ACCTGC ACCTGC(4/8)
716 GCAGGT
BsrDI 177 CATTGC GCAATG(2/0)
3339 CATTGC
BstAPI 1491 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2490 GCACCGGCTGC
Bsu36I 837 CCTGAGG CC^T[ACGT]AGG
1846 CCTGAGG
CelII 1212 GCTGAGC GC^T[ACGT]AGC
1734 GCTCAGC
DrdI 3100 GACAGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3459 GACAGAGGCGTC
EarI 226 CTCTTC CTCTTC(1/4)
3004 CTCTTC
Eco81I 837 CCTGAGG CC^T[ACGT]AGG
1846 CCTGAGG
EcoNI 603 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3129 CCTAGCCAAGG
HincII 510 GTCGAC GT[CT]^[GA]AC
1750 GTTGAC
HindII 510 GTCGAC GT[CT]^[GA]AC
1750 GTTGAC
KasI 2293 GGCGCC G^GCGCC
2401 GGCGCC
Ksp632I 226 CTCTTC CTCTTC(1/4)
3004 CTCTTC
NaeI 659 GCCGGC GCC^GGC
1092 GCCGGC
NarI 2293 GGCGCC GG^CGCC
2401 GGCGCC
NgoMIV 659 GCCGGC G^CCGGC
1092 GCCGGC
PpuMI 1841 GGGACCC [GA]G^G[AT]CC[CT]
1871 AGGACCC
PshAI 1933 GACCCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3412 GACAAATGTC
RcaI 1982 TCATGA T^CATGA
3440 TCATGA
SexAI 2186 ACCTGGT A^CC[AT]GGT
2352 ACCAGGT
SfoI 2293 GGCGCC GGC^GCC
2401 GGCGCC
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# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XhoII