Restriction map for
pFN21AE5509
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
Acc65I AccI AccIII AcsI AfaI
AflII AgeI AocI ApoI AscI
Asp700I Asp718I AspI AviII AvrII
BanI BbeI BbrPI BbvCI BclI
BfaI BfrI BglI BglII BlnI
BsaAI BsaBI BsaWI BseAI BseRI
BsiWI BsmBI BsmI Bsp1407I BspEI
BspHI BsrBI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CpoI DraIII DrdI
EagI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HinfI
HpaI KasI KpnI MaeI MamI
MfeI MluI MroI MunI NarI
NcoI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PvuI RcaI RsaI RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TfiI Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AatII 1207 GACGTC GACGT^C
AclI 679 AACGTT AA^CGTT
AcyI 1207 GACGTC G[GA]^CG[CT]C
AflIII 992 ACATGT A^C[GA][CT]GT
Alw44I 194 GTGCAC G^TGCAC
AlwNI 311 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1253 AGCGCT AGC^GCT
ApaI 1165 GGGCCC GGGCC^C
ApaLI 194 GTGCAC G^TGCAC
AseI 1018 ATTAAT AT^TAAT
AsnI 1018 ATTAAT AT^TAAT
AspHI 194 GTGCAC G[AT]GC[AT]^C
AvaI 1160 CCCGGG C^[CT]CG[GA]G
BalI 138 TGGCCA TGG^CCA
BamHI 1179 GGATCC G^GATCC
BcgI 5 CGACCGCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 662 GGATAC GTATCC(6/5)
BlpI 1026 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1026 GCTCAGC GC^T[ACGT]AGC
BsaHI 1207 GACGTC G[GA]^CG[CT]C
BsaI 1005 GGTCTC GGTCTC(1/5)
BsiEI 5 CGACCG CG[GA][CT]^CG
BsiHKAI 194 GTGCAC G[AT]GC[AT]^C
BsoBI 1160 CCCGGG C^[CT]CG[GA]G
BspDI 717 ATCGAT AT^CGAT
BspLU11I 992 ACATGT A^CATGT
BsrFI 971 GCCGGC [GA]^CCGG[CT]
BssHII 15 GCGCGC G^CGCGC
CelII 1026 GCTCAGC GC^T[ACGT]AGC
Cfr10I 971 GCCGGC [GA]^CCGG[CT]
ClaI 717 ATCGAT AT^CGAT
DdeI 1027 CTCAG C^T[ACGT]AG
DraI 892 TTTAAA TTT^AAA
EaeI 138 TGGCCA [CT]^GGCC[GA]
Eco47III 1253 AGCGCT AGC^GCT
EcoNI 1032 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1001 CCAAGG C^C[AT][AT]GG
FauI 47 GCGGG CCCGC(4/6)
Hin1I 1207 GACGTC G[GA]^CG[CT]C
KspI 8 CCGCGG CCGC^GG
MluNI 138 TGGCCA TGG^CCA
MscI 138 TGGCCA TGG^CCA
MslI 1017 CATTAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 971 GCCGGC GCC^GGC
NdeI 641 CATATG CA^TATG
NgoMIV 971 GCCGGC G^CCGGC
NspI 992 ACATGT [GA]CATG^[CT]
PflMI 1104 CCATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 619 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1018 ATTAAT AT^TAAT
Psp1406I 679 AACGTT AA^CGTT
PvuII 1081 CAGCTG CAG^CTG
SacII 8 CCGCGG CCGC^GG
SapI 487 GAAGAGC GCTCTTC(1/4)
SmaI 1160 CCCGGG CCC^GGG
SspI 867 AATATT AAT^ATT
StyI 1001 CCAAGG C^C[AT][AT]GG
TaqI 718 TCGA T^CGA
Tsp45I 230 GTCAC ^GT[GC]AC
TthHB8I 718 TCGA T^CGA
Van91I 1104 CCATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1160 CCCGGG C^CCGGG
XmaI 1160 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 9 CGCG CG^CG
16 CGCG
AhdI 51 GACGGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
428 GACTGAATGTC
AspEI 51 GACGGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
428 GACTGAATGTC
BanII 1054 GGGCTC G[GA]GC[CT]^C
1165 GGGCCC
BbsI 856 GAAGAC GAAGAC(2/6)
1233 GAAGAC
BpmI 1059 CTCCAG CTGGAG(16/14)
323 CTGGAG
BpuAI 856 GAAGAC GAAGAC(2/6)
1233 GAAGAC
BsgI 1238 CTGCAC GTGCAG(16/14)
460 GTGCAG
BspMI 55 GCAGGT ACCTGC(4/8)
760 GCAGGT
BsrDI 468 CATTGC GCAATG(2/0)
134 GCAATG
BstUI 9 CGCG CG^CG
16 CGCG
BstYI 372 GGATCT [GA]^GATC[CT]
1179 GGATCC
DsaI 8 CCGCGG C^C[GA][CT]GG
1050 CCGTGG
Eam1105I 51 GACGGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
428 GACTGAATGTC
HaeII 301 GGCGCT [GA]GCGC^[CT]
1253 AGCGCT
HgaI 900 GACGC GACGC(5/10)
1153 GCGTC
MaeII 680 ACGT A^CGT
1208 ACGT
MaeIII 230 GTCAC ^GT[ACGT]AC
932 GTTAC
MflI 372 GGATCT [GA]^GATC[CT]
1179 GGATCC
MvnI 9 CGCG CG^CG
16 CGCG
Tsp509I 275 AATT ^AATT
1270 AATT
XhoII 372 GGATCT [GA]^GATC[CT]
1179 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI Ksp632I
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI Tru9I
TseI TspRI