Restriction map for
pFN21AB7847
##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AgeI Alw44I ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BalI BbrPI BbvCI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MseI MunI NcoI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 202 CGCG CG^CG
AflIII 1555 ACATGT A^C[GA][CT]GT
AhdI 34 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 858 CCTGAGG CC^T[ACGT]AGG
Aor51HI 161 AGCGCT AGC^GCT
Asp700I 1230 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 34 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 137 CTCGGG C^[CT]CG[GA]G
AvaII 647 GGTCC G^G[AT]CC
BamHI 547 GGATCC G^GATCC
BanI 414 GGCGCC G^G[CT][GA]CC
BbeI 414 GGCGCC GGCGC^C
BfaI 5 CTAG C^TAG
BglI 100 GCCTTGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 364 GAGGAG GAGGAG(10/8)
BsoBI 137 CTCGGG C^[CT]CG[GA]G
BspLU11I 1555 ACATGT A^CATGT
BsrBI 357 GAGCGG CCGCTC(-3/-3)
BstAPI 444 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 433 GGTGACC G^GT[ACGT]ACC
BstPI 433 GGTGACC G^GT[ACGT]ACC
BstUI 202 CGCG CG^CG
Bsu36I 858 CCTGAGG CC^T[ACGT]AGG
Eam1105I 34 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 161 AGCGCT AGC^GCT
Eco81I 858 CCTGAGG CC^T[ACGT]AGG
EcoO65I 433 GGTGACC G^GT[ACGT]ACC
EcoRI 1329 GAATTC G^AATTC
EcoT14I 774 CCTTGG C^C[AT][AT]GG
HgaI 34 GACGC GACGC(5/10)
HindIII 823 AAGCTT A^AGCTT
KasI 414 GGCGCC G^GCGCC
MaeI 5 CTAG C^TAG
MvnI 202 CGCG CG^CG
NarI 414 GGCGCC GG^CGCC
NdeI 1124 CATATG CA^TATG
PpuMI 646 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 1077 GAGCTC GAGCT^C
SfoI 414 GGCGCC GGC^GCC
SmlI 485 CTCAAG C^T[CT][GA]AG
StuI 999 AGGCCT AGG^CCT
StyI 774 CCTTGG C^C[AT][AT]GG
TaqI 1575 TCGA T^CGA
TthHB8I 1575 TCGA T^CGA
XmnI 1230 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 1271 AAATTC [GA]^AATT[CT]
1329 GAATTC
AcyI 34 GACGCC G[GA]^CG[CT]C
414 GGCGCC
ApaI 141 GGGCCC GGGCC^C
195 GGGCCC
ApoI 1271 AAATTC [GA]^AATT[CT]
1329 GAATTC
BcgI 223 CGAGAACTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
955 CGACATTATTGC
BcnI 183 CCCGG CC^[GC]GG
316 CCGGG
BsaHI 34 GACGCC G[GA]^CG[CT]C
414 GGCGCC
BsaI 491 GGTCTC GGTCTC(1/5)
768 GGTCTC
BsmFI 803 GGGAC GGGAC(10/14)
648 GTCCC
BspMI 420 GCAGGT ACCTGC(4/8)
914 GCAGGT
BsrDI 870 CATTGC GCAATG(2/0)
619 GCAATG
BsrFI 215 GCCGGC [GA]^CCGG[CT]
411 GCCGGC
BstXI 576 CCAGGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
966 CCATGAATGTGG
BstYI 272 AGATCC [GA]^GATC[CT]
547 GGATCC
CfoI 162 GCGC GCG^C
415 GCGC
Cfr10I 215 GCCGGC [GA]^CCGG[CT]
411 GCCGGC
DpnII 273 GATC ^GATC
548 GATC
HaeII 161 AGCGCT [GA]GCGC^[CT]
414 GGCGCC
HhaI 162 GCGC GCG^C
415 GCGC
Hin1I 34 GACGCC G[GA]^CG[CT]C
414 GGCGCC
HinP1I 162 GCGC G^CGC
415 GCGC
MboI 273 GATC ^GATC
548 GATC
MflI 272 AGATCC [GA]^GATC[CT]
547 GGATCC
MspA1I 400 CAGCTG C[AC]G^C[GT]G
706 CAGCTG
NaeI 215 GCCGGC GCC^GGC
411 GCCGGC
NciI 183 CCCGG CC^[GC]GG
316 CCGGG
NdeII 273 GATC ^GATC
548 GATC
NgoMIV 215 GCCGGC G^CCGGC
411 GCCGGC
NspI 888 ACATGC [GA]CATG^[CT]
1555 ACATGT
PleI 1103 GAGTC GAGTC(4/5)
1396 GAGTC
PvuII 400 CAGCTG CAG^CTG
706 CAGCTG
Sau3AI 273 GATC ^GATC
548 GATC
Tsp509I 1272 AATT ^AATT
1330 AATT
XhoII 272 AGATCC [GA]^GATC[CT]
547 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
BanII BbsI BbvI BmyI BpmI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboII MnlI MslI
MspI MvaI MwoI NlaIII NlaIV
PstI Sau96I ScrFI SfaNI SfcI
TfiI TseI Tsp45I TspRI