##################################
# NO CUT ENZYMES (  197 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmBI       BsmFI       BsoBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssKI       BssSI       
Bst1107I    BstBI       BstEII      BstNI       BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DdeI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HaeII       HaeIII      HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HphI        KasI        KpnI        KspI        MaeII       
MamI        MflI        MluI        MluNI       MroI        
MscI        MspA1I      MvaI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        ScrFI       SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
StyI        SwaI        TspRI       Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AciI          431  CCGC                 CCGC(-3/-1)
AfaI          311  GTAC                 GT^AC
BanI          240  GGCACC               G^G[CT][GA]CC
BanII         427  GAGCCC               G[GA]GC[CT]^C
BbvI          447  GCTGC                GCAGC(8/12)
BmyI          427  GAGCCC               G[AGT]GC[ACT]^C
BpmI          118  CTGGAG               CTGGAG(16/14)
BsgI          170  GTGCAG               GTGCAG(16/14)
BsmI          480  GAATGC               GAATGC(1/-1)
Bsp1286I      427  GAGCCC               G[AGT]GC[ACT]^C
BspHI         221  TCATGA               T^CATGA
BsrDI         347  GCAATG               GCAATG(2/0)
BsrI           82  CCAGT                ACTGG(1/-1)
BstAPI        136  GCAAAACTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EarI          207  GAAGAG               CTCTTC(1/4)
FauI          430  CCCGC                CCCGC(4/6)
Fnu4HI        447  GCTGC                GC^[ACGT]GC
HapII          12  CCGG                 C^CGG
HpaII          12  CCGG                 C^CGG
ItaI          447  GCTGC                GC^[ACGT]GC
Ksp632I       207  GAAGAG               CTCTTC(1/4)
MaeIII         31  GTGAC                ^GT[ACGT]AC
MfeI          359  CAATTG               C^AATTG
MslI          227  CACTAGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI           12  CCGG                 C^CGG
MunI          359  CAATTG               C^AATTG
NheI          132  GCTAGC               G^CTAGC
NlaIV         240  GGCACC               GG[ACGT]^[ACGT]CC
NspI          333  GCATGT               [GA]CATG^[CT]
PleI          352  GAGTC                GAGTC(4/5)
PstI          448  CTGCAG               CTGCA^G
RcaI          221  TCATGA               T^CATGA
RsaI          311  GTAC                 GT^AC
SfaNI         436  GATGC                GCATC(5/9)
SspI           43  AATATT               AAT^ATT
TaqI           17  TCGA                 T^CGA
TseI          447  GCTGC                G^C[AT]GC
Tsp45I         31  GTGAC                ^GT[GC]AC
Tsp509I       360  AATT                 ^AATT
TthHB8I        17  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AlwI           14  GGATC                GGATC(4/5)
              189  GGATC               
BsmAI         210  GAGAC                GTCTC(1/5)
              397  GAGAC               
BstF5I        111  GGATG                GGATG(2/0)
              232  GGATG               
FokI          111  GGATG                GGATG(9/13)
              232  GGATG               
MboII         207  GAAGA                GAAGA(8/7)
              405  GAAGA               
MwoI          136  GCAAAACTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              163  GCTGTAGGTGC         
TfiI           66  GAATC                G^A[AT]TC
              201  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AluI        BfaI        Cac8I       DpnII       HinfI       
MaeI        MboI        MnlI        MseI        NdeII       
NlaIII      Sau3AI      SfcI        Tru9I