Restriction map for
pFN21AE1418
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbrPI
BbsI BbvCI BcgI BcnI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BspEI BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT14I EcoT22I FauI FseI FspI
HapII HgaI HincII HindII HindIII
HpaI HpaII KpnI KspI MaeIII
MamI MfeI MluI MroI MspI
MunI MvnI NaeI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StyI
SwaI Tsp45I Tth111I Van91I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 319 AAATTT [GA]^AATT[CT]
AcyI 1054 GGCGCC G[GA]^CG[CT]C
ApoI 319 AAATTT [GA]^AATT[CT]
AvaI 97 CTCGAG C^[CT]CG[GA]G
BalI 1073 TGGCCA TGG^CCA
BamHI 968 GGATCC G^GATCC
BanI 1054 GGCGCC G^G[CT][GA]CC
BanII 408 GAGCCC G[GA]GC[CT]^C
BbeI 1054 GGCGCC GGCGC^C
BciVI 928 GGATAC GTATCC(6/5)
BclI 425 TGATCA T^GATCA
BfaI 778 CTAG C^TAG
BsaAI 726 CACGTA [CT]AC^GT[GA]
BsaHI 1054 GGCGCC G[GA]^CG[CT]C
BsaI 915 GGTCTC GGTCTC(1/5)
BseRI 836 GAGGAG GAGGAG(10/8)
BsmI 819 GAATGC GAATGC(1/-1)
BsoBI 97 CTCGAG C^[CT]CG[GA]G
BspDI 569 ATCGAT AT^CGAT
BstF5I 656 GGATG GGATG(2/0)
BstYI 968 GGATCC [GA]^GATC[CT]
CfoI 1055 GCGC GCG^C
ClaI 569 ATCGAT AT^CGAT
DsaI 999 CCACGG C^C[GA][CT]GG
EarI 601 GAAGAG CTCTTC(1/4)
EcoRV 378 GATATC GAT^ATC
FbaI 425 TGATCA T^GATCA
FokI 656 GGATG GGATG(9/13)
HaeII 1054 GGCGCC [GA]GCGC^[CT]
HhaI 1055 GCGC GCG^C
Hin1I 1054 GGCGCC G[GA]^CG[CT]C
HinP1I 1055 GCGC G^CGC
KasI 1054 GGCGCC G^GCGCC
Ksp632I 601 GAAGAG CTCTTC(1/4)
MaeI 778 CTAG C^TAG
MflI 968 GGATCC [GA]^GATC[CT]
MluNI 1073 TGGCCA TGG^CCA
MscI 1073 TGGCCA TGG^CCA
MspA1I 1085 CAGCTG C[AC]G^C[GT]G
NarI 1054 GGCGCC GG^CGCC
PaeR7I 97 CTCGAG C^TCGAG
PpuMI 1211 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 1085 CAGCTG CAG^CTG
SfoI 1054 GGCGCC GGC^GCC
SmlI 97 CTCGAG C^T[CT][GA]AG
SspI 483 AATATT AAT^ATT
XhoI 97 CTCGAG C^TCGAG
XhoII 968 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 608 CCGC CCGC(-3/-1)
859 GCGG
AspHI 751 GTGCTC G[AT]GC[AT]^C
784 GAGCAC
BsiHKAI 751 GTGCTC G[AT]GC[AT]^C
784 GAGCAC
BsmAI 698 GAGAC GTCTC(1/5)
916 GTCTC
Bsp1407I 168 TGTACA T^GTACA
288 TGTACA
BspHI 203 TCATGA T^CATGA
428 TCATGA
BspLU11I 171 ACATGT A^CATGT
979 ACATGT
BsrGI 168 TGTACA T^GTACA
288 TGTACA
BsrI 1092 ACTGG ACTGG(1/-1)
273 CCAGT
BstXI 522 CCATGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
773 CCAGCCTAGTGG
DraII 532 GGGGCCT [GA]G^G[ACGT]CC[CT]
1211 AGGTCCC
EaeI 680 CGGCCA [CT]^GGCC[GA]
1073 TGGCCA
EcoO109I 532 GGGGCCT [GA]G^G[ACGT]CC[CT]
1211 AGGTCCC
HphI 113 TCACC GGTGA(8/7)
873 TCACC
MaeII 727 ACGT A^CGT
1066 ACGT
MboII 216 GAAGA GAAGA(8/7)
601 GAAGA
MslI 414 CACCCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1065 CACGTCTATG
NspI 171 ACATGT [GA]CATG^[CT]
979 ACATGT
RcaI 203 TCATGA T^CATGA
428 TCATGA
SexAI 470 ACCTGGT A^CC[AT]GGT
1008 ACCTGGT
SfaNI 508 GCATC GCATC(5/9)
560 GCATC
SfcI 26 CTATAG C^T[GA][CT]AG
551 CTGTAG
SspBI 168 TGTACA T^GTACA
288 TGTACA
StuI 491 AGGCCT AGG^CCT
1023 AGGCCT
TaqI 98 TCGA T^CGA
570 TCGA
TfiI 332 GAATC G^A[AT]TC
1195 GATTC
TthHB8I 98 TCGA T^CGA
570 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AfaI AflIII AluI AlwI AvaII
BbvI BglI BmyI BpmI BsaJI
BsgI BsiYI BslI BsmFI Bsp1286I
BsrDI BssKI BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
HinfI ItaI MboI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
Tru9I TseI Tsp509I TspRI XcmI