Restriction map for
pFN21AE2412
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# NO CUT ENZYMES ( 177 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanII BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmAI
BsmBI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DpnII DraI
DraII DraIII DrdI DsaI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MaeI
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MspA1I
MunI MvnI NcoI NdeI NdeII
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tru9I TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcyI 109 GGCGCC G[GA]^CG[CT]C
AluI 480 AGCT AG^CT
AvaII 98 GGACC G^G[AT]CC
BanI 109 GGCGCC G^G[CT][GA]CC
BbeI 109 GGCGCC GGCGC^C
BbsI 331 GTCTTC GAAGAC(2/6)
BpmI 124 CTGGAG CTGGAG(16/14)
BpuAI 331 GTCTTC GAAGAC(2/6)
BsaHI 109 GGCGCC G[GA]^CG[CT]C
BsmFI 22 GTCCC GGGAC(10/14)
BsrDI 388 CATTGC GCAATG(2/0)
BstAPI 378 GCATGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 66 GGTCACC G^GT[ACGT]ACC
BstPI 66 GGTCACC G^GT[ACGT]ACC
CfoI 110 GCGC GCG^C
DdeI 421 CTGAG C^T[ACGT]AG
EaeI 5 CGGCCG [CT]^GGCC[GA]
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
EcoO65I 66 GGTCACC G^GT[ACGT]ACC
HaeII 109 GGCGCC [GA]GCGC^[CT]
HgaI 16 GCGTC GACGC(5/10)
HhaI 110 GCGC GCG^C
Hin1I 109 GGCGCC G[GA]^CG[CT]C
HinP1I 110 GCGC G^CGC
HinfI 129 GATTC G^A[ACGT]TC
HphI 68 TCACC GGTGA(8/7)
KasI 109 GGCGCC G^GCGCC
MaeII 446 ACGT A^CGT
MslI 204 CATGTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 109 GGCGCC GG^CGCC
NspI 251 GCATGT [GA]CATG^[CT]
SfaNI 283 GATGC GCATC(5/9)
SfoI 109 GGCGCC GGC^GCC
SmaI 62 CCCGGG CCC^GGG
TfiI 129 GATTC G^A[AT]TC
XmaCI 62 CCCGGG C^CCGGG
XmaI 62 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaI 62 CCCGGG C^[CT]CG[GA]G
105 CTCGGG
BsaJI 61 CCCCGG C^C[ACGT][ACGT]GG
62 CCCGGG
BseRI 165 CTCCTC GAGGAG(10/8)
238 CTCCTC
BsiEI 5 CGGCCG CG[GA][CT]^CG
523 CGACCG
BsiYI 240 CCTCTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
401 CCATAACCGGG
BslI 240 CCTCTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
401 CCATAACCGGG
BsoBI 62 CCCGGG C^[CT]CG[GA]G
105 CTCGGG
BsrFI 7 GCCGGC [GA]^CCGG[CT]
79 GCCGGC
BsrI 290 ACTGG ACTGG(1/-1)
432 CCAGT
BstNI 123 CCTGG CC^[AT]GG
300 CCTGG
Cfr10I 7 GCCGGC [GA]^CCGG[CT]
79 GCCGGC
Cfr13I 82 GGCCC G^G[ACGT]CC
98 GGACC
EcoRII 123 CCTGG ^CC[AT]GG
300 CCTGG
HaeIII 6 GGCC GG^CC
82 GGCC
MaeIII 67 GTCAC ^GT[ACGT]AC
217 GTGAC
MboII 515 GAAGA GAAGA(8/7)
332 TCTTC
MvaI 123 CCTGG CC^[AT]GG
300 CCTGG
MwoI 7 GCCGGCGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
378 GCATGATTTGC
NaeI 7 GCCGGC GCC^GGC
79 GCCGGC
NgoMIV 7 GCCGGC G^CCGGC
79 GCCGGC
Sau96I 82 GGCCC G^G[ACGT]CC
98 GGACC
Tsp45I 67 GTCAC ^GT[GC]AC
217 GTGAC
Tsp509I 279 AATT ^AATT
459 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI BbvI BcnI BssKI BstF5I
Cac8I FauI Fnu4HI FokI HapII
HpaII ItaI MnlI MspI NciI
NlaIII NlaIV ScrFI TseI