Restriction map for
pFN21AE1432
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# NO CUT ENZYMES ( 186 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BbvI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseRI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
Fnu4HI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HphI ItaI KasI KpnI
Ksp632I KspI MamI MfeI MflI
MluI MspA1I MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfcI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TseI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 566 CCGC CCGC(-3/-1)
AfaI 59 GTAC GT^AC
AlwI 104 GATCC GGATC(4/5)
BalI 116 TGGCCA TGG^CCA
BanI 205 GGTGCC G^G[CT][GA]CC
BbsI 471 GAAGAC GAAGAC(2/6)
BfaI 83 CTAG C^TAG
BpuAI 471 GAAGAC GAAGAC(2/6)
BsgI 179 GTGCAG GTGCAG(16/14)
BsrDI 488 GCAATG GCAATG(2/0)
BsrI 462 ACTGG ACTGG(1/-1)
BssKI 528 CCTGG ^CC[ACGT]GG
BstBI 188 TTCGAA TT^CGAA
BstF5I 419 GGATG GGATG(2/0)
BstNI 528 CCTGG CC^[AT]GG
Cfr13I 250 GGGCC G^G[ACGT]CC
EaeI 116 TGGCCA [CT]^GGCC[GA]
EcoRII 528 CCTGG ^CC[AT]GG
EcoT14I 87 CCTTGG C^C[AT][AT]GG
FokI 419 GGATG GGATG(9/13)
HindIII 456 AAGCTT A^AGCTT
MaeI 83 CTAG C^TAG
MaeII 278 ACGT A^CGT
MaeIII 428 GTTAC ^GT[ACGT]AC
MluNI 116 TGGCCA TGG^CCA
MscI 116 TGGCCA TGG^CCA
MslI 391 CACAGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 528 CCTGG CC^[AT]GG
NlaIII 397 CATG CATG^
NlaIV 205 GGTGCC GG[ACGT]^[ACGT]CC
NspI 396 GCATGC [GA]CATG^[CT]
NspV 188 TTCGAA TT^CGAA
PleI 574 GACTC GAGTC(4/5)
RsaI 59 GTAC GT^AC
Sau96I 250 GGGCC G^G[ACGT]CC
ScaI 58 AGTACT AGT^ACT
ScrFI 528 CCTGG CC^[ACGT]GG
SfaNI 481 GATGC GCATC(5/9)
SfuI 188 TTCGAA TT^CGAA
SphI 396 GCATGC GCATG^C
StuI 240 AGGCCT AGG^CCT
StyI 87 CCTTGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 315 TCCGGA T^CCGGA
416 TCCGGA
AcsI 184 GAATTT [GA]^AATT[CT]
325 GAATTT
AluI 145 AGCT AG^CT
457 AGCT
ApoI 184 GAATTT [GA]^AATT[CT]
325 GAATTT
BsaJI 87 CCTTGG C^C[ACGT][ACGT]GG
230 CCGAGG
BsaWI 315 TCCGGA [AT]^CCGG[AT]
416 TCCGGA
BseAI 315 TCCGGA T^CCGGA
416 TCCGGA
BspEI 315 TCCGGA T^CCGGA
416 TCCGGA
BstXI 107 CCAGCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
285 CCAGCAAGATGG
DpnII 104 GATC ^GATC
373 GATC
HapII 316 CCGG C^CGG
417 CCGG
HpaII 316 CCGG C^CGG
417 CCGG
MboI 104 GATC ^GATC
373 GATC
MboII 322 GAAGA GAAGA(8/7)
471 GAAGA
MroI 315 TCCGGA T^CCGGA
416 TCCGGA
MseI 409 TTAA T^TAA
583 TTAA
MspI 316 CCGG C^CGG
417 CCGG
NdeII 104 GATC ^GATC
373 GATC
Sau3AI 104 GATC ^GATC
373 GATC
TaqI 125 TCGA T^CGA
189 TCGA
TfiI 79 GATTC G^A[AT]TC
133 GATTC
Tru9I 409 TTAA T^TAA
583 TTAA
TspRI 111 CACTG CA[GC]TG[ACGT][ACGT]^
517 CAGTG
TthHB8I 125 TCGA T^CGA
189 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
Cac8I DdeI HaeIII HinfI MnlI
Tsp509I