Restriction map for
pFN21AE9662
##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
AhdI Alw44I ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BbrPI BbsI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BsmBI
BsmI Bsp1407I BspDI BspHI BspMI
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI HphI KspI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI RcaI RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tsp509I TthHB8I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
###################################
Acc65I 904 GGTACC G^GTACC
AccIII 567 TCCGGA T^CCGGA
AcyI 203 GGCGCC G[GA]^CG[CT]C
AflIII 1028 ACATGT A^C[GA][CT]GT
AlwNI 1051 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 871 AGCGCT AGC^GCT
ApaI 755 GGGCCC GGGCC^C
Asp718I 904 GGTACC G^GTACC
AvaI 880 CCCGGG C^[CT]CG[GA]G
BalI 1022 TGGCCA TGG^CCA
BamHI 728 GGATCC G^GATCC
BbeI 203 GGCGCC GGCGC^C
BbvCI 412 GCTGAGG CCTCAGC(-5/-2)
BfaI 703 CTAG C^TAG
BglI 430 GCCCAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 97 TACGTG [CT]AC^GT[GA]
BsaHI 203 GGCGCC G[GA]^CG[CT]C
BseAI 567 TCCGGA T^CCGGA
BsiEI 338 CGATCG CG[GA][CT]^CG
BsiWI 321 CGTACG C^GTACG
BsmAI 549 GTCTC GTCTC(1/5)
BsoBI 880 CCCGGG C^[CT]CG[GA]G
BspEI 567 TCCGGA T^CCGGA
BspLU11I 1028 ACATGT A^CATGT
BsrDI 170 CATTGC GCAATG(2/0)
BssHII 292 GCGCGC G^CGCGC
BstXI 512 CCAAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 728 GGATCC [GA]^GATC[CT]
DraIII 217 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 109 GACGCAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 871 AGCGCT AGC^GCT
EcoT22I 991 ATGCAT ATGCA^T
Hin1I 203 GGCGCC G[GA]^CG[CT]C
KasI 203 GGCGCC G^GCGCC
KpnI 904 GGTACC GGTAC^C
MaeI 703 CTAG C^TAG
MflI 728 GGATCC [GA]^GATC[CT]
MluNI 1022 TGGCCA TGG^CCA
MroI 567 TCCGGA T^CCGGA
MscI 1022 TGGCCA TGG^CCA
MseI 242 TTAA T^TAA
NaeI 44 GCCGGC GCC^GGC
NarI 203 GGCGCC GG^CGCC
NcoI 941 CCATGG C^CATGG
NgoMIV 44 GCCGGC G^CCGGC
NsiI 991 ATGCAT ATGCA^T
NspI 1028 ACATGT [GA]CATG^[CT]
PflMI 216 CCACGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 338 CGATCG CGAT^CG
PvuII 113 CAGCTG CAG^CTG
SapI 867 GAAGAGC GCTCTTC(1/4)
ScaI 840 AGTACT AGT^ACT
SexAI 824 ACCAGGT A^CC[AT]GGT
SfaNI 993 GCATC GCATC(5/9)
SfcI 479 CTGTAG C^T[GA][CT]AG
SfoI 203 GGCGCC GGC^GCC
SmaI 880 CCCGGG CCC^GGG
Tru9I 242 TTAA T^TAA
TspRI 390 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 216 CCACGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 728 GGATCC [GA]^GATC[CT]
XmaCI 880 CCCGGG C^CCGGG
XmaI 880 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 313 GTCTAC GT^[AC][GT]AC
774 GTCTAC
AlwI 729 GATCC GGATC(4/5)
728 GGATC
AocI 349 CCTCAGG CC^T[ACGT]AGG
453 CCTCAGG
AspI 220 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
823 GACCAGGTC
BsaWI 567 TCCGGA [AT]^CCGG[AT]
598 TCCGGT
BseRI 415 GAGGAG GAGGAG(10/8)
706 GAGGAG
BsgI 811 CTGCAC GTGCAG(16/14)
1057 CTGCAC
BsrFI 44 GCCGGC [GA]^CCGG[CT]
677 ACCGGC
BssSI 831 CACGAG CACGAG(-5/-1)
7 CTCGTG
BstF5I 228 CATCC GGATG(2/0)
994 CATCC
Bsu36I 349 CCTCAGG CC^T[ACGT]AGG
453 CCTCAGG
Cfr10I 44 GCCGGC [GA]^CCGG[CT]
677 ACCGGC
DpnII 339 GATC ^GATC
729 GATC
DsaI 194 CCGTGG C^C[GA][CT]GG
941 CCATGG
EarI 55 GAAGAG CTCTTC(1/4)
867 GAAGAG
Eco81I 349 CCTCAGG CC^T[ACGT]AGG
453 CCTCAGG
EcoT14I 512 CCAAGG C^C[AT][AT]GG
941 CCATGG
FokI 228 CATCC GGATG(9/13)
994 CATCC
HgaI 109 GACGC GACGC(5/10)
449 GCGTC
Ksp632I 55 GAAGAG CTCTTC(1/4)
867 GAAGAG
MboI 339 GATC ^GATC
729 GATC
MslI 690 CACTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
984 CACTAAAATG
NdeII 339 GATC ^GATC
729 GATC
PflFI 220 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
823 GACCAGGTC
PpuMI 131 AGGACCC [GA]G^G[AT]CC[CT]
345 GGGACCT
SacI 179 GAGCTC GAGCT^C
268 GAGCTC
Sau3AI 339 GATC ^GATC
729 GATC
StyI 512 CCAAGG C^C[AT][AT]GG
941 CCATGG
Tsp45I 562 GTGAC ^GT[GC]AC
829 GTCAC
Tth111I 220 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
823 GACCAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrBI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII EaeI EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeII MaeIII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PleI RsaI
Sau96I ScrFI TfiI TseI