Restriction map for
pFN21AE6417
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BsaAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI HindIII HpaI KpnI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI SapI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI Tth111I Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Asp700I 432 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 542 GGATCC G^GATCC
BbeI 119 GGCGCC GGCGC^C
BsiEI 1083 CGACCG CG[GA][CT]^CG
BspLU11I 434 ACATGT A^CATGT
BspMI 1576 ACCTGC ACCTGC(4/8)
BsrBI 1606 GAGCGG CCGCTC(-3/-3)
BstAPI 996 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EarI 1536 GAAGAG CTCTTC(1/4)
FseI 1272 GGCCGGCC GGCCGG^CC
HaeII 119 GGCGCC [GA]GCGC^[CT]
HgaI 266 GCGTC GACGC(5/10)
KasI 119 GGCGCC G^GCGCC
Ksp632I 1536 GAAGAG CTCTTC(1/4)
KspI 984 CCGCGG CCGC^GG
MseI 1382 TTAA T^TAA
NaeI 1273 GCCGGC GCC^GGC
NarI 119 GGCGCC GG^CGCC
NgoMIV 1273 GCCGGC G^CCGGC
PpuMI 112 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1671 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1256 CAGCTG CAG^CTG
SacII 984 CCGCGG CCGC^GG
SbfI 993 CCTGCAGG CCTGCA^GG
SfoI 119 GGCGCC GGC^GCC
SmaI 1445 CCCGGG CCC^GGG
SmlI 108 CTTGAG C^T[CT][GA]AG
SphI 9 GCATGC GCATG^C
Sse8387I 993 CCTGCAGG CCTGCA^GG
StuI 1550 AGGCCT AGG^CCT
TaqI 1082 TCGA T^CGA
TfiI 966 GAATC G^A[AT]TC
Tru9I 1382 TTAA T^TAA
Tsp509I 138 AATT ^AATT
TthHB8I 1082 TCGA T^CGA
XmaCI 1445 CCCGGG C^CCGGG
XmaI 1445 CCCGGG C^CCGGG
XmnI 432 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 223 TCCGGA T^CCGGA
428 TCCGGA
AcyI 119 GGCGCC G[GA]^CG[CT]C
265 GGCGTC
AfaI 282 GTAC GT^AC
474 GTAC
AflIII 35 ACGTGT A^C[GA][CT]GT
434 ACATGT
ApaI 728 GGGCCC GGGCC^C
1569 GGGCCC
AvaI 616 CCCGAG C^[CT]CG[GA]G
1445 CCCGGG
BalI 1016 TGGCCA TGG^CCA
1206 TGGCCA
BbsI 676 GTCTTC GAAGAC(2/6)
976 GTCTTC
BfaI 254 CTAG C^TAG
451 CTAG
BlpI 401 GCTCAGC GC^T[ACGT]AGC
1031 GCTCAGC
Bpu1102I 401 GCTCAGC GC^T[ACGT]AGC
1031 GCTCAGC
BpuAI 676 GTCTTC GAAGAC(2/6)
976 GTCTTC
BsaBI 186 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
543 GATCCTCATC
BsaHI 119 GGCGCC G[GA]^CG[CT]C
265 GGCGTC
BsaWI 223 TCCGGA [AT]^CCGG[AT]
428 TCCGGA
BseAI 223 TCCGGA T^CCGGA
428 TCCGGA
BsoBI 616 CCCGAG C^[CT]CG[GA]G
1445 CCCGGG
BspEI 223 TCCGGA T^CCGGA
428 TCCGGA
BspHI 395 TCATGA T^CATGA
770 TCATGA
BsrDI 825 CATTGC GCAATG(2/0)
1341 CATTGC
BsrFI 1085 ACCGGC [GA]^CCGG[CT]
1273 GCCGGC
BstYI 542 GGATCC [GA]^GATC[CT]
836 AGATCC
CelII 401 GCTCAGC GC^T[ACGT]AGC
1031 GCTCAGC
Cfr10I 1085 ACCGGC [GA]^CCGG[CT]
1273 GCCGGC
Hin1I 119 GGCGCC G[GA]^CG[CT]C
265 GGCGTC
HincII 1183 GTCAAC GT[CT]^[GA]AC
1348 GTTGAC
HindII 1183 GTCAAC GT[CT]^[GA]AC
1348 GTTGAC
MaeI 254 CTAG C^TAG
451 CTAG
MamI 186 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
543 GATCCTCATC
MflI 542 GGATCC [GA]^GATC[CT]
836 AGATCC
MluNI 1016 TGGCCA TGG^CCA
1206 TGGCCA
MroI 223 TCCGGA T^CCGGA
428 TCCGGA
MscI 1016 TGGCCA TGG^CCA
1206 TGGCCA
NcoI 692 CCATGG C^CATGG
1286 CCATGG
NspI 9 GCATGC [GA]CATG^[CT]
434 ACATGT
PstI 994 CTGCAG CTGCA^G
1238 CTGCAG
RcaI 395 TCATGA T^CATGA
770 TCATGA
RsaI 282 GTAC GT^AC
474 GTAC
SfcI 994 CTGCAG C^T[GA][CT]AG
1238 CTGCAG
XcmI 561 CCAGGAGGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1121 CCACCATCAACATGG
XhoII 542 GGATCC [GA]^GATC[CT]
836 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaII BanI BanII BbvI
BcnI BglI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI SacI Sau3AI
Sau96I ScrFI SfaNI StyI TseI
Tsp45I TspRI